Peculiarities of Formation and Functioning of the Soybean–bradyrhizobium Japonicum Symbiotic Apparatus in Relation to Photosynthetic Activity Under the Influence of Seed Protectants

Kots, Serhii; Kiriziy, D.A.; Pavlyshche, A.V.; Rybachenko, L. I.

doi:10.55251/jmbfs.3128

Cited by 3 publications

(3 citation statements)

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“…For soybean plants and other legumes, studies revealed close correlation between the processes of formation of biological productivity and nitrogen-fixing activity of their symbiotic systems, including those the seeds of which had been treated with fungicides (Pavlyshche et al, 2018;Silva et al, 2018;Hungria еt аl., 2020;Kots еt аl., 2022). Activation of intensity of nitrogen-fixation of symbiotic system after spraying plants with specific lectin (Table 3), which we determined in the study, may have been caused, first, of all by indirect action of photosynthetic apparatus and metabolism of products of photosynthetic activity of plants, which are a source of energy for functioning of the nitrogenase complex (Kots et al, 2022), because earlier we had determined participation of lectins in coordination of processes of nitrogen-fixation and photosynthesis in phytobacterial systems after exogenous influence on seeds (Sytnikov et al, 2006;Kyrychenko, 2014), and, secondly, by possible direct action of lectin on auxin / сytokinin balance of plants, as indicated after exogenous effect of wheat germ agglutinin on wheat seeds (Kyrychenko, 2014).…”

Section: Discussionmentioning

confidence: 94%

“…Treatment seeds of soybean should be correlated with another technological method that is necessarily used in technology of its cultivationseed inoculation with highly active and effective strains of rhizobia. Seed inoculation with rhizobia results in development of symbiotic apparatus, the fundamental function of which is absorption of molecular nitrogen (Ciampitti & Salvagiotti, 2018), thereby promoting better realization of productive potential of plants (Silva et al, 2018;Hungria et al, 2020;Rodrigues et al, 2020;Kots et al, 2022). Fixation of atmospheric nitrogen by soybean-rhizobial symbiotic systems promotes intensification of arable farming, decrease in energy expenditures, improvement of ecological condition of soils, as well as provision of high level of grain productivity of soybean (Ciampitti & Salvagiotti, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, we know that against the background of using fungicides, development of plants and formation of their biological and seed productivities significantly changed, and nitrogenase activity of legume-rhizobial symbioses significantly weakened, especially in the first half of the vegetation (Mostoviak & Kravchenko, 2019;Gorshkov et al, 2020;Rodrigues et al, 2020;Kots et al, 2022). Under in situ conditions against the background of using fungicides Maksim Star 025 FS and Kinto Duo, symbio-tic systems of soybean altered their functional activity, leading to decrease in the share of biological nitrogen in yield.…”

Section: Introductionmentioning

confidence: 99%

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Biological activity of soybean seed lectin at the spraying of Glycine max plants against the background of seed treatment with pesticide containing fipronil, thiophanate-methyl, pyraclostrobin as active substances and rhizobial bacterization

Kyrychenko

Kots

Khrapova

et al. 2022

Regul. Mech. Biosyst.

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Preparations for protecting plants, particularly those with fungicidal activity, continue to be relevant in agricultural production. They are used to effectively combat phytopathogens and ensure high yield of cultivated plants. However, they are among the anthropogenic factors which impose a heavy chemical load on ecosystems. Data about the effects of pesticides on physiological parameters of plants are essential for understanding the main regulatory mechanisms as preconditions to the phytotoxic state of compounds, as well as greater substantial understanding of the functional condition and implementation of adaptive potential of plants during and post stress. An important and relevant task – both practically and theoretically in the conditions of action of fungicide on seeds - is studying the possibilities of application of phytolectines as biologically active compounds with broad spectrum of action, including fungicidal effect, for spraying legumes in order to stabilize their development, ensure effective functioning of legume-rhizobial symbioses and cause fuller realization of productive potential against the background of decrease in chemical pressure on agrocenoses. Therefore, we aimed to evaluate the biological activity of soybean seed lectin (according to the parameters of productivity and functional activity of soybean-rhizobial symbiosis) at the spraying of Glycine max (L.) Merr. plants against the background of seed treatment of pesticide, Standak Top with fungicidal and insecticidal actions on the day of sowing and inoculation with Bradyrhizobium japonicum 634b. We used physiological, biochemical, microbiological and statistical methods of studies. We determined that Standak Top, applied on soybeans that were afterwards inoculated with rhizobia, exerted negative tendency on formation of vegetative mass by plants at the beginning of vegetation, though in the following phases of ontogenesis, their development and productivity reached the level of the control. Nitrogenase activity of symbiosis and the condition of photosynthetic pigment complex (content of chlorophyll and carotenoids and their ratio) were at the level or significantly lower than in the control plants. After spraying soybeans with lectin (without use of the fungicide) in the phase of development of two true leaves, there occurred significant increase in functional activity of the symbiotic system (according to total nitrogenase activity of symbiosis, higher by 1.91 and 1.79 times compared with the controls with inoculation and inoculation + fungicide) and the content of photosynthetic pigments (chlorophylls were higher by 1.12–1.45 times, carotenoids by 1.14–1.39 times) and development of strong leaf apparatus (by 1.33–1.42 times). This caused highest level of realization (by 13.9% and 10.1% higher compared with the controls with inoculation and inoculation + fungicide) of productive potential of cultivated plants. After spraying plants with soybean lectin against the background of use of fungicide, notable and reliable increases occurred in the level of absorption of molecular nitrogen (by 1.72 and 1.52 times according to total activity of symbiosis, compared with the controls with inoculation and inoculation + fungicide), content of chlorophyll (1.25–1.64 times) and carotenoids (1.12–1.42 times) in leaves of soybean, and also plants were actively developing during vegetation (1.12–1.40 times), producing yield that exceeded by 12.8% and 9.1% the controls with inoculation and inoculation + fungicide. Therefore, use of soybean seed lectin for spraying plants against the background of seed treatment of pesticide Standak Top on day of sowing can stabilize and even increase the level of realization of symbiotic and productive potential of soybean-rhizobial symbiosis compared both with the control (inoculation with rhizobia) and the variant with treatment of seeds (rhizobia + fungicide). This indicates on the perspectives of further studies of biological activity of phytolectins aiming at decreasing chemical pressure on ecosystems by leveling out or decreasing the negative impact of chemical means of protection on the plants and symbiosis.

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Section: Discussionmentioning

confidence: 94%