Peculiarities in the formation, structural-mechanical properties of a fibrin-blood clot and its importance for bone regeneration in fractures

Popsuishapka, Olexey; Litvishko, Valeriy; Ashukina, Nataliya; Danishchuk, Zinayda

doi:10.15674/0030-5987201345-12

Cited by 3 publications

(5 citation statements)

References 3 publications

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“…Among the mechanical factors, the surface pressure is important. It was proved that on the surface of the contact with the trabecular layer which causes pressure, a layer of dense fibrin forms, and in the central part -its structure is cellular (Popsuishapka et al, 2013). A similar structure of fibrin clot was determined in microscopic examination of the biopsy material received from experimental animals (Popsuishapka et al, 2015).…”

Section: Discussionmentioning

confidence: 92%

“…In vitro studies have proved that the structural organization of fibrin-blood clots is affected by mechanical and chemical factors (Popsuishapka et al, 2013;Wang et al, 2016). Among the mechanical factors, the surface pressure is important.…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have different opinions on this topic. Some focus on the inflammatory as the factor which initiates the process (Marsell & Einhom, 2012), other focus on the role of hematoma (Schell et al, 2017) or on the process of formation of the fibrin-collagen carcass (Popsuishapka et al, 2013;Echeverri et al, 2015;Wang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Fibrin-blood clot as an initial stage of formation of bone regeneration after a bone fracture

Popsuishapka

Ashukina

Litvishko³

et al. 2018

Regul. Mech. Biosyst.

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This study focuses on mechanisms which regulate the process of fracture healing. We studied the form and position of the fibrin-blood clots (FBC) in the zone near the fragments among patients with limb fractures: macroscopically (during open surgical operations to reposition bone fragments) and using sonography. We conducted a histological and immuno-histochemical analysis of biopsy material obtained from the zone around the fracture during surgical procedures on 16 patients in 1–18 days after the fracture. We determined that the density of FBC and their form depends on the volume of damage to the periosteum-muscular fascia and the extent of the shift of fragments. In most cases, with a closed fracture, fibrin-blood clots had a spindle-shaped form. Fibrin along the periphery and in the zone between the fragments has a dense structure, and becomes cellular in central zones. The cells surrounded by fibrin partitions contain blood cells and serum. In many places, fibrin partitions had a one-direction orientation position, and the cells were oval-elongated, which indicated hydrostatic pressure in them. Proliferation of mesenchymal cells began in the vital tissues around the FBC, then during reproduction they pentrated to fibrin. Lengthwise axis of the cells was parallel to fibrin partitions. The bone trabeculae which form on the third week after fracture repeated the orientation of the fibrin partitions. It was determined that the vascular endothelial growth factor (VGEF) concentrates in fibrin and remains in it over the first week after the fracture, later it was found in endotheliocytes, fibroblasts and osteoblasts. The process of filling of the cells with around-fracture FBC lasted 12–18 days and during this period, their osteogenic differentiation occurred. Such tempi and orientation of the process is caused by fibrin with a concentration of growth factors in it. Using the results of the study, it could be assumed that the main conditions for osteogenic differentiation of cells are high concentration of VGEF in the fibrin, which initiates neoangiogenesis and internal tension of fibrin partitions. The formation of structured FBC around the ends of the fragments, which contain VGEF should be considered an initial stage of the process of forming of bone regeneration.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Fibrin-blood clot as an initial stage of formation of bone regeneration after a bone fracture

Popsuishapka

Ashukina

Litvishko³

et al. 2018

Regul. Mech. Biosyst.

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“…Його активність зумовлена вмістом біологічно активних факторів, які вивільнюються з тромбоцитів у процесі коагуляції крові [5]. Раніше ми з'ясували, що утворення згустку відбувається впродовж 12-24 год після травми, його форма і розташування залежать від величини зміщення відламків та розриву окісном'язового футляру [4,8]. Найсприятливіші умови для фібриноутворення та подальшої регенерації виникають за переломів з незначним зміщенням, коли навколовідламковий простір має невеликий об'єм і обмежений цілим (або майже цілим) окісном'язовим футляром.…”

Section: The Article Is Prepared For Practical Traumatologists Threeunclassified

“…Решта порожнини заповнена сироваткою крові з еритроцитами. Встановлено, що перемішування крові прискорює утворення фібрину і забезпечує рівномірний розподіл його волокон по всьому об'єму [4]. Припускають, що збереження тиску крові в порожнині також сприяє полімеризації фібриногену.…”

Section: The Article Is Prepared For Practical Traumatologists Threeunclassified

The functional treatment of the diaphyseal tibial fractures using plaster cast or external fixator

Litvishko¹,

Popsuishapka²

2015

ORTHOPAEDICS, TRAUMATOLOGY and PROSTHETICS

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Ключові слова: діафізарні переломи гомілки, функціональне лікуванння, гіпсова пов'язка, апарати зовнішьої фіксації Нещодавно автори провели епідеміологічне дослідження результатів лікування діафізарних переломів довгих кісток за матеріалами Харківської міжрайонної МСЕК протягом 2008-2010 рр. [7].

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Bone fragments displacement in the treatment of diaphyseal fractures and their importance to the process of bone regeneration

Popsuishapka¹,

Litvishko²,

Ashukina³

et al. 2016

ORTHOPAEDICS, TRAUMATOLOGY and PROSTHETICS

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Peculiarities in the formation, structural-mechanical properties of a fibrin-blood clot and its importance for bone regeneration in fractures

Cited by 3 publications

References 3 publications

Fibrin-blood clot as an initial stage of formation of bone regeneration after a bone fracture

Fibrin-blood clot as an initial stage of formation of bone regeneration after a bone fracture

The functional treatment of the diaphyseal tibial fractures using plaster cast or external fixator

Bone fragments displacement in the treatment of diaphyseal fractures and their importance to the process of bone regeneration

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