Peculiar features of the Mycobacterium tuberculosis population structure in Albania

Tafaj, Silva; Mokrousov, Igor; Borroni, Emanuele; Trovato, Alberto; Kapisyzi, Perlat; Bardhi, Donika; Hafizi, Hasan; Bala, Silvana; Bulo, Anyla; Bino, Silvia; Rastogi, Nalin; Cirillo, Daniela María

doi:10.1016/j.meegid.2019.104136

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…However, more recently it became clear that this situation varies in different countries and likely depends on the national control programmes. For example, LAM‐RUS is mainly drug‐susceptible in Albania, similar to other M. tuberculosis genotypes in that country (Tafaj et al., 2020). In Latvia, not only Beijing SIT1 but also LAM SIT42 were overrepresented among MDR strains (Pole et al., 2020).…”

Section: Resultsmentioning

confidence: 92%

Spatiotemporal dynamics of drug‐resistant Mycobacterium tuberculosis : Contrasting trends and implications for tuberculosis control in EU high‐priority country

Mokrousov

Vyazovaya

Levina

et al. 2020

Transbounding Emerging Dis

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Different and contrasting trends related to human migration and the implementation of health control programmes influence the spread of drug-resistant tuberculosis (TB). We analysed the Mycobacterium tuberculosis population structure in Estonia, a high-priority EU country for TB control, to detect the dynamic changes and underlying factors. The study collection included 278 M. tuberculosis isolates recovered in 1999 and 2014-2015. The isolates were subjected to drug susceptibility testing, genotyping and analysis of sublineage/cluster-specific markers and drug resistance mutations. The Beijing genotype was the most prevalent, and its rate increased from 28.6% in 1999 to 38.5% in 2015 (p = .09). The non-Beijing strains represented Euro-American lineage (Latin American Mediterranean [LAM], Ural, Haarlem, T, X genotypes) and Indo-Oceanic lineage (one EAI-IND isolate). The proportion of isolates resistant to two or more drugs increased from 22.4% to 29.1% (p = .1). The pre-XDR/ XDR isolates were identified only within the Beijing genotype. In contrast, the drug resistance rate decreased in the LAM genotype from 42.1% to 11.8% (p = .05). The Beijing B0/W148-cluster ('successful Russian strain') included only MDR, pre-XDR or XDR isolates. All B0/W148-cluster isolates were resistant to two or more drugs compared to 28% of the Beijing 94-32-cluster (p = .0002). The Beijing genotype was not identified in the isolates from patients born in Estonia before 1940 compared to its 35.2% rate among other patients. In summary, the circulation of the highly drugresistant isolates of the Beijing B0/W148 subtype, the increased prevalence of the Beijing genotype among HIV-coinfected patients and the increased number of patients with alcohol abuse (47.5%) present major challenges of the current TB control in Estonia. The Beijing genotype was likely brought to Estonia after 1945 due to the massive human influx from the Soviet Union. In contrast, the main genotypes of the Euro-American lineage were likely endemic in Estonia during all 20th century.

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Section: Resultsmentioning

confidence: 92%

Spatiotemporal dynamics of drug‐resistant Mycobacterium tuberculosis : Contrasting trends and implications for tuberculosis control in EU high‐priority country

Mokrousov

Vyazovaya

Levina

et al. 2020

Transbounding Emerging Dis

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“…Outside FSU, the Beijing 1071-32 isolates were described in Serbia 31 , Albania 32 and Greece 33 . These are Balkan countries but whether this fact reflects an already local circulation of this strain rather than independent importations from FSU is presently unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The three isolates from Greece were one from an immigrant from Georgia (FSU) and the remainder two from local Greek patients 33 . Moreover, Beijing genotype is extremely rare in Albania 32 and it was previously speculated that these isolates in Albania could be linked to neighboring Greece. A large European multicenter study of drug resistant TB in the EU ten years ago identified the Beijing 1071-32 isolates and designated them as European resistant cluster ECDC_10 34 .…”

Section: Discussionmentioning

confidence: 99%

Practical approach to detection and surveillance of emerging highly resistant Mycobacterium tuberculosis Beijing 1071-32-cluster

Mokrousov

Vyazovaya

Sinkov

et al. 2021

Sci Rep

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Ancient sublineage of the Mycobacterium tuberculosis Beijing genotype is endemic and prevalent in East Asia and rare in other world regions. While these strains are mainly drug susceptible, we recently identified a novel clonal group Beijing 1071-32 within this sublineage emerging in Siberia, Russia and present in other Russian regions. This cluster included only multi/extensive drug resistant (MDR/XDR) isolates. Based on the phylogenetic analysis of the available WGS data, we identified three synonymous SNPs in the genes Rv0144, Rv0373c, and Rv0334 that were specific for the Beijing 1071-32-cluster and developed a real-time PCR assay for their detection. Analysis of the 2375 genetically diverse M. tuberculosis isolates collected between 1996 and 2020 in different locations (European and Asian parts of Russia, former Soviet Union countries, Albania, Greece, China, Vietnam, Japan and Brazil), confirmed 100% specificity and sensitivity of this real-time PCR assay. Moreover, the epidemiological importance of this strain and the newly developed screening assay is further stressed by the fact that all identified Beijing 1071-32 isolates were found to exhibit MDR genotypic profiles with concomitant resistance to additional first-line drugs due to a characteristic signature of six mutations in rpoB450, rpoC485, katG315, katG335, rpsL43 and embB497. In conclusion, this study provides a set of three concordant SNPs for the detection and screening of Beijing 1071-32 isolates along with a validated real-time PCR assay easily deployable across multiple settings for the epidemiological tracking of this significant MDR cluster.

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Ubiquitous and multifaceted: SIT53 spoligotype does not correlate with any particular family of Mycobacterium tuberculosis

Mokrousov

2021

Tuberculosis

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Peculiar features of the Mycobacterium tuberculosis population structure in Albania

Cited by 4 publications

References 19 publications

Spatiotemporal dynamics of drug‐resistant Mycobacterium tuberculosis : Contrasting trends and implications for tuberculosis control in EU high‐priority country

Spatiotemporal dynamics of drug‐resistant Mycobacterium tuberculosis : Contrasting trends and implications for tuberculosis control in EU high‐priority country

Practical approach to detection and surveillance of emerging highly resistant Mycobacterium tuberculosis Beijing 1071-32-cluster

Ubiquitous and multifaceted: SIT53 spoligotype does not correlate with any particular family of Mycobacterium tuberculosis

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