Pectolinarigenin regulates the tumor-associated proteins in AGS-xenograft BALB/c nude mice

Abstract

Background Pectolinarigenin (PEC) is one of the flavonoid active ingredients, and the anti-inflammatory and anti-cancer effects of PEC are being studied continuously. In a previous study, we found out the PEC regulates PI3K/AKT/mTOR pathway on gastric cancer (GC) cells. The purpose of this study was to establish the GC regulatory function of PEC in vivo and in vitro. Methods In this research, PEC was intraperitoneally administered to confirm tumor control after xenografting the AGS cell line into BALB/c nude mice to form tumors. Subsequently, tumors were extracted and LC-MS analysis and Gene ontology were performed for proteomics. Results The body weight and hematological analysis showed that PEC was not toxic to the non-cancerous cells in the xenograft mice model. We identified 582 proteins related to cellular responses such as tumorigenesis and cell death siganling in the tumor tissue. 6 out of 582 proteins was regulation similar by PEC in vivo and in vitro. Conclusion Our findings indicated that PEC treatment might suppress tumor growth in GC, and proteomic analysis provides the basic information about proteins that could be of significant potential as novel therapeutic targets againsg GC.