Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae

Lukianova, Anna A.; Evseev, Peter; Shneider, Mikhail M.; Dvoryakova, Elena A.; Tokmakova, Anna; Shpirt, Anna M.; Kabilov, Мarsel R.; Obraztsova, Ekaterina A.; Shashkov, Alexander S.; Игнатов, А. Н.; Knirel, Yuriy A.; Dzhalilov, F.S.; Miroshnikov, Konstantin A.

doi:10.3390/ijms231911043

Cited by 5 publications

(1 citation statement)

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“…These mutants remained sensitive to some of the O-antigen-dependent (strategy II) phages such as phiKT and Alt63. More recently, other phages harboring spikes with deacetylase activity towards OPS [49,50] and capsular polysaccharides of enterobacteria were discovered. These viruses may also select for two distinct phenotypes of resistant mutants.…”

Section: Strategies Of Host Cell Recognition By Bacteriophages and Pr...mentioning

confidence: 99%

Bacterial Virus Forcing of Bacterial O-Antigen Shields: Lessons from Coliphages

Letarov

2023

IJMS

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In most Gram-negative bacteria, outer membrane (OM) lipopolysaccharide (LPS) molecules carry long polysaccharide chains known as the O antigens or O polysaccharides (OPS). The OPS structure varies highly from strain to strain, with more than 188 O serotypes described in E. coli. Although many bacteriophages recognize OPS as their primary receptors, these molecules can also screen OM proteins and other OM surface receptors from direct interaction with phage receptor-binding proteins (RBP). In this review, I analyze the body of evidence indicating that most of the E. coli OPS types robustly shield cells completely, preventing phage access to the OM surface. This shield not only blocks virulent phages but also restricts the acquisition of prophages. The available data suggest that OPS-mediated OM shielding is not merely one of many mechanisms of bacterial resistance to phages. Rather, it is an omnipresent factor significantly affecting the ecology, phage–host co-evolution and other related processes in E. coli and probably in many other species of Gram-negative bacteria. The phages, in turn, evolved multiple mechanisms to break through the OPS layer. These mechanisms rely on the phage RBPs recognizing the OPS or on using alternative receptors exposed above the OPS layer. The data allow one to forward the interpretation that, regardless of the type of receptors used, primary receptor recognition is always followed by the generation of a mechanical force driving the phage tail through the OPS layer. This force may be created by molecular motors of enzymatically active tail spikes or by virion structural re-arrangements at the moment of infection.

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Section: Strategies Of Host Cell Recognition By Bacteriophages and Pr...mentioning

confidence: 99%