Pectobacterium aroidearum sp. nov., a soft rot pathogen with preference for monocotyledonous plants

Abstract

Several pectolytic bacterial strains, mainly isolated from monocotyledonous plants and previously identified as Pectobacterium carotovorum, were thought to belong to a novel species after several taxonomic analyses including DNA-DNA hybridization. In 16S rRNA gene sequence analyses, these strains had a similarity of .97.9 % to the 16S rRNA gene sequence of strains representing six other pectobacterial species and subspecies. These strains, represented by strain SCRI 109 T , also showed some unique chemotaxonomic features and quantitative differences in polar lipids, lipoquinones and fatty acids. A specific feature of strain SCRI 109 T was the presence of DMK-8 lipoquinone, while the dominant fatty acids were the summed feature 3 (iso-C 15 : 0 2-OH/ C 16 : 1 v7c), the unsaturated fatty acid C 18 : 1 v7c and straight chain fatty acids, mainly C 16 : 0 . The DNA G+C content of strain SCRI 109 T was 50.2 mol%. The taxonomic status of strain SCRI 109 T and related strains in 16S rRNA gene sequence, chemotaxonomic, and physiological analyses was corroborated by the distinct clustering of these strains in multi-locus sequence analyses. It is proposed that these strains represent a novel species for which the name Pectobacterium aroidearum sp. nov. is proposed; the type strain is SCRI 109 T (5NCPPB 929 T 5LMG 2417 T 5ICMP 1522 T ).

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

“…Two strains that are considered to be type strains of two Pectobacterium species, PC1 (P. aroidearum) and Pcb1692 (P. carotovorum ssp. brasiliense), were used in this study (Nabhan et al, 2013;Onkendi and Moleleki, 2014). Pathogens belonging to these species are able to infect monocot and dicot hosts of agricultural importance, especially under warm conditions (Nabhan et al, 2012(Nabhan et al, , 2013Onkendi and Moleleki, 2014).…”

Effects of plant antimicrobial phenolic compounds on virulence of the genus Pectobacterium

“…Alternatively, the Pectobacterium carotovorum strains PC11 and WPP14, as well as the Pectobacterium aroidearum strain PC1 were more tolerant. The strain PC1 was once considered to belong to P. carotovorum, but was recently re-classified as P. aroidearum, a monocot specializing species [39,47]. This specialization could be at least in part attributed to the high tolerance of this Pectobacterium strain to most of the phenolic compounds.…”

Multiplex detection and identification of bacterial pathogens causing potato blackleg and soft rot in Europe, using padlock probes

“…subsp. carotovorum (Ma et al , ; De Boer et al , ; Nabhan et al , , ). The genus Dickeya is a diverse group consisting of five genomic species, that is, D. zeae , D. paradisiaca, D. dadantii , D. chrysanthemi and D. dianthicola (Samson et al , ; Brady et al , ) and new proposed species ‘ D. solani ’ (Sławiak et al , 2009 b ; Toth et al , ), all except the first two were found in association with potato in Europe (Sławiak et al , 2009 b ; Toth et al , ).…”