Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases

Zheng, Li; Xu, Yinxiao; Li, Qian; Zhu, Benwei

doi:10.1186/s40643-021-00432-z

Cited by 32 publications

(12 citation statements)

References 67 publications

(117 reference statements)

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“…PME randomly hydrolyzes the methylated carboxyl group in polygalacturonic acid to form pectate, the substrate of PG; however, PME can also act on galacturonic acid by a linear block fashion to produce long stretches of negatively charged carboxylates that cross connect with Ca 2+ , resulting in reduced cell wall porosity and the hardening of the cell wall [ 42 , 43 ]. PL acts on the deesterified polygalacturonic acid to form a double bond between C4 and C5 and finally blocks the α-1,4-glycosidic bond to produce pectin oligosaccharides [ 44 ]. PGs are divided into three types: external PGs, internal PGs and rhamnose PGs.…”

Section: Cell Wall Metabolism and Structure Remodelingmentioning

confidence: 99%

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Peng

Liu

et al. 2022

IJMS

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Fruit softening that occurs during fruit ripening and postharvest storage determines the fruit quality, shelf life and commercial value and makes fruits more attractive for seed dispersal. In addition, over-softening results in fruit eventual decay, render fruit susceptible to invasion by opportunistic pathogens. Many studies have been conducted to reveal how fruit softens and how to control softening. However, softening is a complex and delicate life process, including physiological, biochemical and metabolic changes, which are closely related to each other and are affected by environmental conditions such as temperature, humidity and light. In this review, the current knowledge regarding fruit softening mechanisms is summarized from cell wall metabolism (cell wall structure changes and cell-wall-degrading enzymes), plant hormones (ETH, ABA, IAA and BR et al.), transcription factors (MADS-Box, AP2/ERF, NAC, MYB and BZR) and epigenetics (DNA methylation, histone demethylation and histone acetylation) and a diagram of the regulatory relationship between these factors is provided. It will provide reference for the cultivation of anti-softening fruits.

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Section: Cell Wall Metabolism and Structure Remodelingmentioning

confidence: 99%

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Peng

Liu

et al. 2022

IJMS

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“…There are many enzymes associated with pectin degradation in PUL 3, such as PL 1_2, GH43, GH28, CE19 and GH105, among which PL 1_2 and GH43 were significantly up-regulated. The PL 1 family can encode pectin cleavage enzymes, and the GH43 family can encode α-L-arabinosidase [ 42 , 43 ]. PLs could degrade HG into oligosaccharides in order to be transported to the periplasm.…”

Section: Discussionmentioning

confidence: 99%

The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea

Shi,

Zhou,

Chen

et al. 2024

Foods

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In the present study, four Bacteroides species that could degrade Fuzhuan brick tea polysaccharide-3 (FBTPS-3) were isolated from human feces and identified to be Bacteroides ovatus, B. uniformis, B. fragilis and B. thetaiotaomicron. The four Bacteroides species showed growth on FBTPS-3 as the carbon source, and B. ovatus showed the best capability for utilizing FBTPS-3 among the four species since B. ovatus could utilize more FBTPS-3 during 24 h fermentation. Moreover, the four Bacteroides species could metabolize FBTPS-3 and promote the production of acetic, propionic and isovaleric acids. Transcriptome analysis of B. ovatus revealed that 602 genes were up-regulated by FBTPS-3, including two carbohydrate-active enzyme clusters and four polysaccharide utilization loci (PULs). The PUL 1 contained GH28 family that could hydrolyze rhamnogalacturonan and other pectic substrates, which was in line with our previous work that rhamnose and galacturonic acid were the main component monosaccharides of FBTPS-3. Collectively, the results suggested that FBTPS-3 could be utilized by Bacteroides spp., and it might be developed as a promising prebiotic targeting Bacteroidetes in intestinal environment.

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“…Increased use of fungicides in controlling plant diseases has led to several environmental issues, including the growth of resistant weed and pathogen populations, soil compaction and water pollution, which negatively influence agricultural sustainability and human health (Peng et al, 2021). As a result, it is crucial to discover new fungicides that are mostly plant-derived, such as essential oils (EO), to control plant diseases (Zheng et al, 2022).…”

Section: ………………………………… ……… ……………………… Thyme and Clove Essential Oil An...mentioning

confidence: 99%

Antifungal Activity of Thyme and Clove Essential Oil Nanoemulsions against Pothos Root Rot

Attia,

Mohamad,

Baiumy

et al. 2024

Egyptian Journal of Agricultural Sciences

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Fungi, including Rhizoctonia solani and Sclerotium rolfsii, pose a significant threat to agricultural productivity, causing substantial crop yield and quality losses. The excessive use of conventional fungicides raises concerns regarding their environmental impact and human health. This study explores the antifungal potential of thyme and clove oil nanoemulsions as a sustainable alternative. Nanoemulsions were prepared using high-energy ultrasonication and exhibited remarkable stability, with homogenous particle size distributions and low polydispersity index values. In vitro assays demonstrated complete fungal growth inhibition at 3000 ppm for both oils. Pot bioassays revealed significant antifungal activity, with thyme oil nanoemulsion reducing R. solani and S. rolfsii root rot by 75% and 50%, respectively. Clove oil nanoemulsion also effectively suppressed these pathogens by 41.67% and 25%, respectively. Thyme and clove oil nanoemulsions treatments significantly stimulated root growth under fungal challenge. In this regard, plants treated with thyme oil nanoemulsion showed remarkable increases in root length (4.61-fold and 11.18-fold), fresh weight (28.59-fold and 45.62-fold), and dry weight (48.29 and 48.43-fold) when exposed to R. solani and S. rolfsii infection, respectively, compared to untreated control. Clove oil nanoemulsion also significantly enhanced these root attributes, with remarkable increases in length (1.87-fold for R. solani and 4.12-fold for S. rolfsii), fresh weight (11.09fold for R. solani and 21.31-fold for S. rolfsii), and dry weight (25.14-fold for R. solani and 16-fold for S. rolfsii). Molecular modeling studies supported the observed antifungal efficacy. In conclusion, thyme and clove essential oil nanoemulsions are promising candidates that act as natural fungicides for managing root rot diseases.

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Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases

Cited by 32 publications

References 67 publications

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

The Utilization by Bacteroides spp. of a Purified Polysaccharide from Fuzhuan Brick Tea

Antifungal Activity of Thyme and Clove Essential Oil Nanoemulsions against Pothos Root Rot

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