Pectinolytic Enzymes

Jacob, Nicemol

doi:10.1007/978-1-4020-9942-7_21

Cited by 14 publications

(9 citation statements)

References 43 publications

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“…This result confirmed previous findings of highest pectinase production by A. sojae ATCC 20235 in SSF (Heerd et al 2012 ). Highest fungal polygalacturonic acid degrading exo-enzyme activity (480 U/g) measured with the arsenomolybdate reagent in SSF extracts was produced on wheat bran by A. carbonarius (Jacob 2009 ). This enzyme yield was 1.9 times increased using A. sojae ATCC 20235 in SSF under optimized conditions in this study.…”

Section: Resultsmentioning

confidence: 99%

“…Alternatively, high content in pectins (20-25%), its availability and low-cost make sugar beet pulp a potential source of pectins. Sugar beet pulp is mainly composed of (% on dry basis) pectin, 28.7; cellulose, 20; hemicellulose, 17.5; protein, 9.0; and lignin 4.4 (Jacob 2009 ). Since the pectin content of beet pulp or apple pomace is high it can be used for the microbial production of pectinolytic enzymes without adding pectinaceous material as inducer.…”

Section: Introductionmentioning

confidence: 99%

“…Since the pectin content of beet pulp or apple pomace is high it can be used for the microbial production of pectinolytic enzymes without adding pectinaceous material as inducer. However, the most potent solid agro-industrial by-product for biotechnological pectinolytic enzyme production, used in combination or without pectinase inducers, is wheat bran, which is composed predominantly of non-starch carbohydrates, starch and crude proteins (Jacob 2009 ).…”

Section: Introductionmentioning

confidence: 99%

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Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

2014

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Previously identified fungal pectinase producers of the species Aspergillus sojae were used for optimization of polygalacturonase production in solid-state fermentation applying Design of Experiment. The effects of media composition and several process parameters, like inoculum size, moisture level, incubation time and temperature on polygalacturonase activity were studied in screening and optimization investigations. Utilization of agricultural and agro-industrial by-products provided the establishment of a cost-efficient and sustainable process for enzyme production. Comparison of pectinase production by A. sojae ATCC 20235 and A. sojae CBS 100928 under optimized conditions yielded 6.9 times higher polygalacturonase activity by A. sojae ATCC 20235. Highest enzyme yield (909.5 ± 2.7 U/g) was obtained by A. sojae ATCC 20235 after 8 days at 30°C applying 30% sugar beet pulp as inducer substrate in combination with wheat bran as medium wetted at 160% with 0.2 M HCl. Furthermore, an overview of pectinolytic enzyme activities present in the extracts of both strains is provided. Protein profiles of both strains are given by SDS-PAGE electrophoresis, as well as zymograms for pectinolytic enzymes in comparison to commercial pectinase preparations.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-3-742) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

2014

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“…Unmolassed sugar beet pellets (Nordzucker AG, Uelzen, Germany), and apple pomace (Döhler Dienter GmbH, Neuenkirchen, Germany), previously freeze dried, were ground using a small high‐speed mixing system (0.5 L capacity); the apple pomace consisted of a mixture of different kinds of apples such as Elstar, Jonagold, Jonagored and Braeburn. An approximate chemical composition of the carbon sources is presented in Table . Powdered carbon sources with heterogeneous particle size were used in combination with fine wheat bran (90% < 630 µm) supplied by Bremer Rolandmühle Erling GmbH & Co. KG (Bremen, Germany).…”

Section: Methodsmentioning

confidence: 99%

A novel pectin‐degrading enzyme complex from Aspergillus sojaeATCC 20235 mutants

et al. 2014

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“…Pectinases are a group of enzymes that contribute to the degradation of pectin, which is a complex acidic polysaccharide present in the primary cell wall and middle lamella of higher plant tissues. The significance of these enzymes for the development of environment friendly industrial processes has already been established [1].…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies of Pectinase Production byBacillus subtilis strain Btk 27in Submerged and Solid-State Fermentations

Oumer

Abate

2018

BioMed Research International

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The request for enzymes in the global market is expected to rise at a fast pace in recent years. With this regard, there has been a great increase in industrial applications of pectinase owing to their significant biotechnological uses. This study was undertaken with main objectives of meeting the growing industrial demands of pectinase, by improving the yield without increasing the cost of production. In addition, this research highlights the underestimated potential of agroresidues for the production of biotechnologically important products. In this study, the maximum pectinase production attained was using wheat bran, among the tested agroresidues. The production of pectinase was improved from 10.1 ± 1.4 U/ml to 66.3 ± 1.2 U/ml in submerged fermentation whereas it was in solid state fermentation from 800.0 ± 16.2 U/g to 1272.4 ± 25.5 U/g. The maximum pectinase production was observed using YEP (submerged fermentation) and wheat bran (solid state fermentation) at initial pH of 6.5, at 37°C and by supplementing the medium with 3 mM MgSO4.7H2O.

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Pectinolytic Enzymes

Cited by 14 publications

References 43 publications

Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

Efficient polygalacturonase production from agricultural and agro-industrial residues by solid-state culture of Aspergillus sojae under optimized conditions

A novel pectin‐degrading enzyme complex from Aspergillus sojaeATCC 20235 mutants

Comparative Studies of Pectinase Production byBacillus subtilis strain Btk 27in Submerged and Solid-State Fermentations

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