Pectinase-treated Panax ginseng extract (GINST) rescues testicular dysfunction in aged rats via redox-modulating proteins

Won, Young Jun; Shin, Yong‐Kyu; Jung, Seung Hyo; Yoo, Sung-Kwang; Hwang, Seock-Yeon; Sung, Jong-Hwan; Kim, Si-Kwan

doi:10.1016/j.exger.2014.02.012

Cited by 24 publications

(32 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many sex hormones such as FSH, LH and androgens are vital in the process of germ cell apoptosis and survival (Xu et al 2016). However, as described in our previous studies, GINST treatment attenuated the declining levels of sex hormones (Won et al 2014, Kopalli et al 2016 and the sex hormone receptors. In agreement with these findings, heat stress lowered the levels of sex hormone receptors, and consequently, increased germ cell apoptosis.…”

Section: Figuresupporting

confidence: 58%

“…The GINST dose (100 and 200 mg/kg b.w./day) was based on our previously reported study (Won et al 2014). Each GINST dose (100 and 200 mg/ kg/day) was mixed separately with a regular pellet diet and was administered orally for 8 weeks starting from 1 week before heat exposure.…”

Section: Experimental Designmentioning

confidence: 99%

“…For the in vivo analysis, total RNA was extracted from the testicular tissue using the RNA-Bee reagent according to the manufacturer's instructions, and the RNA (1 μg) was reversetranscribed following the procedures as described previously (Won et al 2014). The primers used were listed in Table 1.…”

Section: Rna Isolation and Real-time Reverse Transcription Polymerasementioning

confidence: 99%

“…A Chinese study revealed several genes associated with teratospermia that were also potentially associated with sperm quality (Liu et al 2016). Many clinical studies revealed its effect in improving several diseases including hypertension, diabetes, Alzheimer's disease, acquired immune deficiency syndrome, cancer and sexual dysfunction (Vogler et al 1999, Hwang et al 2004, Du et al 2011, Ramesh et al 2012, Yayeh et al 2012, Won et al 2014, Kopalli et al 2015. Among the factors that may disrupt these processes, heat stress is common.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pectinase-treated Panax ginseng protects heat stress-induced testicular damage in rats

Kim

Cha²,

Hwang³

et al. 2017

Reproduction

Self Cite

View full text Add to dashboard Cite

Testicular hyperthermia is well studied to cause impaired spermatogenesis. In the present study, the protective effect of enzymatically modified (pectinase-treated) (GINST) against intermittent sub-chronic heat stress-induced testicular damage in rats was investigated. Male Sprague-Dawley rats were divided into four groups: normal control (NC), heat-stressed control (HC), heat-stressed plus GINST-100 mg/kg/day (HG100) and heat-stressed plus GINST-200 mg/kg/day (HG200) treatment groups. GINST (100 and 200 mg/kg/day) was mixed separately with a regular pellet diet and was administered orally for 8 weeks starting from 1 week before heat exposure. Parameters such as organ weight, blood chemistry, sperm kinetic values, expression of antioxidant enzymes, spermatogenesis molecules and sex hormone receptors levels were measured. Data revealed that kidney and epididymis weight were significantly ( < 0.05) decreased with heat stress and recovered by GINST treatment. Further, the altered levels of blood chemistry panels and sperm kinetic values in heat stress-induced rats were attenuated when GINST was administered ( < 0.05). Furthermore, the expression levels of antioxidant-related enzymes (GSTM5 and GPX4), spermatogenesis-related proteins (CREB1 and INHA) and sex hormone receptors (androgen receptor, luteinizing hormone receptor and follicle-stimulating hormone receptor) were reduced by heat stress; however, GINST treatment effectively ameliorated these changes. In conclusion, GINST was effective in reducing heat-induced damage in various male fertility factors and has considerable potential to be developed as a useful supplement in improving male fertility.

show abstract

Section: Figuresupporting

confidence: 58%

Section: Experimental Designmentioning

confidence: 99%

Section: Rna Isolation and Real-time Reverse Transcription Polymerasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pectinase-treated Panax ginseng protects heat stress-induced testicular damage in rats

Kim

Cha²,

Hwang³

et al. 2017

Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ginseng (Panax ginseng Meyer, Araliaceous) is an important medicinal herb that has long been used to treat various diseases in Asian countries [1] , [2] . In particular, the major active constituents, ginsenosides, are known to have various physiological activities, such as anti-aging and anti-inflammatory effects, and antioxidant effects in the central nervous system, cardiovascular system, reproductive system, and immune system [1] , [3] , [4] . It was suggested that P. ginseng has potent effects on sexual function, and could relieve erectile dysfunction [5] , senile testicular dysfunction [2] , [6] , and dioxin-induced testicular damage [7] .…”

Section: Introductionmentioning

confidence: 99%

Pectinase-treated Panax ginseng ameliorates hydrogen peroxide-induced oxidative stress in GC-2 sperm cells and modulates testicular gene expression in aged rats

Kopalli

Cha

Jeong

et al. 2016

Journal of Ginseng Research

Self Cite

View full text Add to dashboard Cite

BackgroundTo investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models.MethodsHydrogen peroxide (H2O2)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting.ResultsGINST treatment (50 μg/mL, 100 μg/mL, and 200 μg/mL) significantly (p < 0.05) inhibited the H2O2-induced (200 μM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 μg/mL and 100 μg/mL) significantly (p < 0.05) ameliorated the H2O2-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-α, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H2O2-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats.ConclusionGINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.

show abstract

Panax ginseng

Lim¹

2014

Edible Medicinal and Non Medicinal Plants

View full text Add to dashboard Cite

Pectinase-treated Panax ginseng extract (GINST) rescues testicular dysfunction in aged rats via redox-modulating proteins

Cited by 24 publications

References 43 publications

Pectinase-treated Panax ginseng protects heat stress-induced testicular damage in rats

Pectinase-treated Panax ginseng protects heat stress-induced testicular damage in rats

Pectinase-treated Panax ginseng ameliorates hydrogen peroxide-induced oxidative stress in GC-2 sperm cells and modulates testicular gene expression in aged rats

Panax ginseng

Contact Info

Product

Resources

About