Pectinase Activity of Vegetable Spoilage Bacteria in Modified Atmosphere

Abstract

A modified atmosphere of 10% CO*, 5% O2 and 85% Nz was used to determine effects on pectinase activities of vegetable spoilage bacteria. Cultures of Erwinia carotovora, Pseudomonasji'uorescens, and Xanthomonas campesti, grown at 25°C in liquid medium held under the modified atmosphere were assayed for polygalacturonase and pectate lyase activities. Erwinia and Pseudomonas exhibited higher polygalacturonase and pectate lyase activities thanXanthomonas whether grown under modified atmosphere or air. In some cases, bacteria grown under air exhibited higher pectinase activity. In general modified atmosphere did not significantly reduce pectinase activities in any of the bacteria tested.

Potential Application of Hot Rehydration Alone or in Combination with Hydrogen Peroxide to Control Pectin Methylesterase Activity and Microbial Load in Cold‐stored Intermediate‐moisture Sun‐dried Figs

“…The ability of different bacteria and fungi to produce pectic enzymes was reported by different researchers (Liu and Luh 1978;Hao and Brackett 1994). However, the presence of PME enzyme activity in sun-dried figs indicated that the enzyme was not formed by some microorganisms during cold storage of rehydrated IM figs.…”

Mechanism of fungal inhibition activity of Nα‐lauroyl‐L‐arginine ethyl ester (LAE) and potential in control of Penicillium expansum on postharvest citrus ‘Benimadonna’ (Citrus reticulata × Citrus sinensis)

“…However, the research shows that pectinase activity can be affected by various factors such as pH 37 and gas atmosphere. 38 In particular, there is some evidence that upregulation of permeability induced by stress from exogenous compounds in fungal cells can lead to a reduction in their pectinase activity; for instance, cell membrane permeability of Fusarium oxysporum was elevated by neodymium cation, while its pectinase activity was inhibited; in Botrytis cinerea, polygalacturonase activity also decreases with increased permeability in response to phenazine-1-carboxamide treatment; and cuminic acidinduced elevation of cell permeability in Valsa mali was accompanied by an inhibition of its pectinase activity. Based on these findings on the relationship between fungal cell permeability and pectinase activity, a possible mechanism of LAE for pectinase inhibition might be generated through its cell permeability alteration.…”

A review of microbial biofilms of produce: Future challenge to food safety

“…Frequency of diseases, types of pathogens, and produce outbreaks have been revealed in a review by Erickson (16). The frequency and incidence of contamination also depend on extrinsic environmental conditions (17). In Italy, Caponigro et al (18) reported that 27% of samples were found to be E. coli positive amongst 1,158 ready-to-eat vegetables, including lettuce, arugula, spinach, and lamb's lettuce.…”