Pectin from Brassica oleracea var. italica triggers immunomodulating effects in vivo

Busato, Bianca; Abreu, Elaine Cristina de Almeida; Petkowicz, Carmen Lúcia de Oliveira; Martinez, Gláucia Regina; Noleto, Guilhermina Rodrigues

doi:10.1016/j.ijbiomac.2020.06.051

Cited by 22 publications

(9 citation statements)

References 35 publications

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“…This result is in contrast with the work published by Busato et al [57] where the pectin extracted from B. oleracea showed a phagocytic activity of peritoneal macrophage cells increased by 63 %. The phagocytic behavior was also observed by Kuo et al [58] who proposed a different approach.…”

Section: Application Of Cocoa Pods Pectin In Biomedical Fieldcontrasting

confidence: 99%

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Cocoa Pod Husk: A High‐Pectin Source with Applications in the Food and Biomedical Fields

et al. 2022

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Cocoa liquor, butter, and powder represent derived products from a small portion of the fruits, compared with the cocoa pod husk (CPH) which accounts for ~70 % of fresh weight. CPH, improperly disposed in plantations, can cause diseases threatening worldwide chocolate production. However, this biomass can be a potential source of bioactive compounds aligned with the circular economy. An overview on the different methods for extracting pectin, resulting in variable extraction yields with a critical discussion on the obtained physicochemical characteristics, is presented. Additionally, the potential applications of the extracted pectin for food and biomedical application are discussed, including thickener, stabilizer, excipient, drug-release modifier, macrophage activator, etc. Despite these potential outputs, new extraction methods need to be considered for improving efficiency and sustainability. Finally, potential approaches are introduced that can help to minimize the environmental impact, making the extraction cost-and time-efficient, and, therefore, more ssustainable for a further successful translation to industry.

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Section: Application Of Cocoa Pods Pectin In Biomedical Fieldcontrasting

confidence: 99%

“…Essential inputs and outputs of the extraction process related to the six principles of green extraction 57.…”

Section: Current Challenges Of Pectin Extraction From Cocoa and Its T...mentioning

confidence: 99%

Cocoa Pod Husk: A High‐Pectin Source with Applications in the Food and Biomedical Fields

et al. 2022

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“…Pectin was not able to induce cell proliferation. This fact was also noted by Busato and coauthors [40] which verified in pectin obtained from Brassica oleracea did not show cytotoxic effect. Furthermore, it was not able to promote cell proliferation in in vitro assays, at concentrations of 250 and 500µg/mL when compared to the control (ConA).…”

Section: Discussionsupporting

confidence: 77%

Pectin-like Polysaccharide Extracted from the Leaves Caesalpinia pulcherrima is a Promising Antioxidant and Immunomodulator Agent

Melo

Sousa

Silva

et al. 2022

Braz. arch. biol. technol.

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Studies have shown that Caesalpinia pulcherrima extracts promote antioxidant, healing, immunomodulating and antiparasitic activities and its polysaccharides can be used as functional food. In this sense, this work had as objective the isolation and characterization of a polysaccharide-like pectin, extracted from the C. pulcherrima leaves and its possible applications as an antioxidant and immunomodulator agent. The molecule was characterized by high performance liquid chromatography, fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Its antioxidant potential was evaluated through the methods of phosphomolybdenum, ABTS radical scavenging [2,2'-azino-bis (3-ethylbenzothiazoline-6sulfonic acid], DPPH (1,1-diphenyl-2-picrylhydrazyl) and nitric oxide radical. The immunostimulating effects of pectin were tested in splenocytes to evaluate its toxic, proliferative and cell activator and immunomodulatory potential. The polysaccharide obtained has structural characteristics similar to pectins. Pectin showed high in vitro antioxidant activity for ABTS radical scavenging, moderate activity for phosphomolybdenum and low activity for DPPH and nitric oxide. In vitro immunomodulation assays showed that pectin obtained did not promote a cytotoxic effect (viability > 90%). The increase in cytosolic ROS levels indicates a possible mechanism of cell activation without causing damage. Immunophenotyping showed that HIGHLIGHTS• The pectin-like polysaccharide was isolated from the Caesalpinia pulcherrima leaves.• Showed promising results of antioxidant activity in in vitro assays.• The polysaccharide is non-toxic, stimulates cell activation and cytokine production in in vitro.• Promising therapeutic agent.

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“…Similar studies based on β-glucan uptake mechanisms should be more explored for pectins in general, as both are non-digestible carbohydrates that suffer fermentation to an extensive degree [ 2 ]. Modified pectin from broccoli ( Brassica oleracea L. Italica) was suggested to be absorbed after an increased number of activated macrophages and lymphocyte proliferation when administered through oral treatment in mice, but with no same effect in vitro [ 122 ]. One possible suggestion of pectin “absorption” is related to asialoglycoprotein receptors that could play a role in absorbing modified pectin fragments throughout the intestine, as they are notable galactoside-terminal glycoproteins transporters [ 123 , 124 ]; however, much more pieces of evidence need to be described to confirm this hypothesis.…”

Section: Pectin and Gal-3 Controversiesmentioning

confidence: 99%

“…Pectin-derived oligosaccharides with 1 kDa and rich in galactosyl residues were absorbed in BALB/c mice and human tumor cells while also changing membrane permeability in different human cancer cells, such as HepG2 and Colo 205 (hepatic and colon carcinoma, respectively) [ 127 ]. Microfold cells and gut-associated lymphoid tissue (GALT) are also proposed as explanations for the bloodstream presence of modified pectin fragments, where the former theoretically would serve as a facilitator to GALT macrophages to act internalizing these portions [ 122 , 128 ]. Once again, these assumptions of absorption models are still in germinative steps; therefore, they cannot be taken as unreasoning facts, but they can open new potential transcription elucidation of in vivo observed effects towards clinical significance in the future.…”

Section: Pectin and Gal-3 Controversiesmentioning

confidence: 99%

The Complex Biological Effects of Pectin: Galectin-3 Targeting as Potential Human Health Improvement?

2022

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Galectin-3 is the only chimeric representative of the galectin family. Although galectin-3 has ubiquitous regulatory and physiological effects, there is a great number of pathological environments where galectin-3 cooperatively participates. Pectin is composed of different chemical structures, such as homogalacturonans, rhamnogalacturonans, and side chains. The study of pectin’s major structural aspects is fundamental to predicting the impact of pectin on human health, especially regarding distinct molecular modulation. One of the explored pectin’s biological activities is the possible galectin-3 protein regulation. The present review focuses on revealing the structure/function relationship of pectins, their fragments, and their biological effects. The discussion highlighted by this review shows different effects described within in vitro and in vivo experimental models, with interesting and sometimes contradictory results, especially regarding galectin-3 interaction. The review demonstrates that pectins are promissory food-derived molecules for different bioactive functions. However, galectin-3 inhibition by pectin had been stated in literature before, although it is not a fully understood, experimentally convincing, and commonly agreed issue. It is demonstrated that more studies focusing on structural analysis and its relation to the observed beneficial effects, as well as substantial propositions of cause and effect alongside robust data, are needed for different pectin molecules’ interactions with galectin-3.

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Pectin from Brassica oleracea var. italica triggers immunomodulating effects in vivo

Cited by 22 publications

References 35 publications

Cocoa Pod Husk: A High‐Pectin Source with Applications in the Food and Biomedical Fields

Cocoa Pod Husk: A High‐Pectin Source with Applications in the Food and Biomedical Fields

Pectin-like Polysaccharide Extracted from the Leaves Caesalpinia pulcherrima is a Promising Antioxidant and Immunomodulator Agent

The Complex Biological Effects of Pectin: Galectin-3 Targeting as Potential Human Health Improvement?

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