Pectin extraction from citron peel: optimization by Box–Behnken response surface design

Pasandide, Bahare; Khodaiyan, Faramarz; Mousavi, Zeinab E.; Hosseini, Seyed Saeid

doi:10.1007/s10068-018-0365-6

Cited by 69 publications

(40 citation statements)

References 44 publications

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“…According to the previous Plackett — Burman screening test, the optimal conditions of the inoculum size, maize meal, and histidine were determined by the Box–Behnken design. A three‐factor and three‐level second‐order regression of the Box–Behnken design consisting of 17 experimental runs was employed including five replicates at the center point (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…It requires less experimentation to measure the interaction between the test factors. The Box–Behnken design is more time saving, labor saving, and effective, and it can effectively analyze the interactions between various factors .…”

Section: Discussionmentioning

confidence: 99%

“…The Box–Behnken design was used in the Design Expert 7.0 software to evaluate the three factors. Each of these three factors had three coded levels consisting of 17 experimental runs for analyzing the experimental data, including five replicates at the center point (Table ).…”

Section: Experimental Designmentioning

confidence: 99%

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The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

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Xylanase is a member of an important family of enzymes that has been used in many biotechnological processes. However, the overall cost of enzyme production has been the main problem in the industrial application of enzymes. To obtain maximum xylanase production, statistical approaches based on the Plackett-Burman design and response surface methodology were employed. The results of the statistical analyses demonstrated that the optimal conditions for increased xylanase production were the following: inoculum size, 3.8%; maize meal, 4.5%; histidine, 0.6%; methanol, 1%; culture volume, 20%; bean pulp, 30 g L −1 ; and Tween-80, 0.8%; and pH 5.0. Verification of the optimization demonstrated that 3273 U mL −1 xylanase was observed under the optimal conditions in shake flask experiments. SDS-PAGE results showed that the size of xylanase protein was about 23 kDa. The results showed that the xylanase produced by fermentation came from Aspergillus Niger by MALDI-TOF-MS. The optimized medium resulted in 2.1-and 1.4-fold higher the activity of xylanase compared with the unoptimized medium (the main nutrients are maize meal and bean pulp) and laboratory medium (the main nutrients are yeast extract and peptone), respectively.The optimization of fermentation conditions is an effective means to reduce production cost and improve xylanase activity. K E Y W O R D Smethodology optimization, Plackett-Burman, xylanase Abbreviations: PBS, phosphate-buffered saline; RSM, response surface method.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

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“…The strong infrared band near 3300 cm −1 was observed for the O-H stretching vibration of hydroxyl group. The absorption peak at 2914 cm −1 might be induced by C-H stretching vibration of the CH 2 and CH 3 groups (Pasandide et al 2017). The absorption at 1740 cm −1 was caused by C=O stretching vibration, while the absorption at about 1625-1600 cm −1 was due to the C=O asymmetrical stretching vibration of free carboxyl groups typical for the structure of flavonoids.…”

Section: Ftir Analysis Of Silver and Copper Nanoparticlesmentioning

confidence: 95%

Hawthorn berries extract for the green synthesis of copper and silver nanoparticles

2019

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Silver and copper nanoparticles (AgNPs and CuNPs) have attracted worldwide attention due to their unique antimicrobial properties. Ethanol and water extracts of hawthorn fruit were chosen to prepare AgNPs and CuNPs. The effects of the pH, contact time, temperature of the solution, type of precursor of metal ions and type of hawthorn extract on the size distribution of particles were studied. The monodispersed and stable AgNPs and CuNPs were prepared with an average size of 60 nm and 200 nm, respectively. Total phenolic content and the anthocyanin content of extracts were determined. The antimicrobial activities of AgNPs and CuNPs were carried out with a series of microbial assays.

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“…The young tender leaves and fruits of citron watermelon can be cooked as green vegetables, while the mature fruit flesh is mixed with maize meal to prepare porridge [6].…”

Section: Introductionmentioning

confidence: 99%

Citron Watermelon Potential to Improve Crop Diversification and Reduce Negative Impacts of Climate Change

Mandizvo¹,

Odindo²,

Mashilo³

2021

Preprint

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Citron watermelon (Citrullus lanatus var. citroides) is an underexploited and under-researched crop species with potential to contribute to crop diversification in sub-Saharan Africa and beyond. The species is commonly cultivated in the drier parts of Southern Africa, mainly by smallholder farmers who maintain a wide range of landraces. Understanding the molecular and morpho-physiological basis for drought adaptation of Citron watermelon in these dry environments can aid in screening local germplasm, identification of suitable traits for crop improvement and improving food system resilience among smallholder farmers by adding to crop diversification. This paper reviews literature on drought adaptation of C. lanatus spp. (C3 xerophytes), using the systematic review approach. The review discusses; (i) the potential role of citron watermelon in adding to crop diversification, (ii) alternative food uses and potential by-products that can be processed from citron watermelon and (iii) the role of Sub-Saharan farmers as key actors in conserving citron watermelon germplasm and biodiversity. Finally, the review provides a summary of significant findings and identifies critical knowledge gaps for further research.

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Pectin extraction from citron peel: optimization by Box–Behnken response surface design

Cited by 69 publications

References 44 publications

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Hawthorn berries extract for the green synthesis of copper and silver nanoparticles

Citron Watermelon Potential to Improve Crop Diversification and Reduce Negative Impacts of Climate Change

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