Pectin Esterase in Relation to Leaf Abscission in <i>Coleus</i> and <i>Phaseolus</i>

LaMotte, Clifford E.; Gochnauer, Carl; LaMotte, Lynn Roy; Mathur, J. R.; Davies, Leslie L. R.

doi:10.1104/pp.44.1.21

Cited by 11 publications

(16 citation statements)

References 20 publications

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“…S4B), so it is likely that the protein product from this gene may be secreted. Although a role for pectin esterases in abscission was first proposed over 50 years ago (Osborne, 1958), the evidence remains conflicting (Yager, 1960;LaMotte et al, 1969;Ratner et al, 1969;Moline et al, 1972;Roberts and González-Carranza, 2009). In Arabidopsis, it has been demonstrated that three of at least 59 putative pectin esterase genes in Arabidopsis are up-regulated during floral organ abscission (Cai and Lashbrook, 2008).…”

Section: Proposed Roles For the Abscission-related Genes Identifiedmentioning

confidence: 99%

“…The abscission process has been extensively studied, and many of the analyses have been performed using excised segments of tissue (or explants) exposed to nonphysiological concentrations of plant regulators such as ethylene or auxin for sustained periods of time (from a few hours to several days; Osborne, 1958;LaMotte et al, 1969;Sexton and Roberts, 1982;Coupe et al, 1995;González-Carranza et al, 2002;Belfield et al, 2005;Tucker et al, 2007). Such an approach makes the precise cellular composition of the AZ difficult to establish, and to add to this complication, analyses of molecular changes occurring during the process is potentially confused by "contamination" from neighboring nonseparating tissues.…”

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confidence: 99%

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A Novel Approach to Dissect the Abscission Process in Arabidopsis

et al. 2012

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Abscission is the consequence of a specialized layer of cells undergoing a complex series of molecular and biochemical events. Analysis of the specific molecular changes associated with abscission is hampered by contamination from neighboring nonseparating tissues. Moreover, studies of abscission frequently involve the examination of events that take place in isolated segments of tissue exposed to nonphysiological concentrations of ethylene or indole-3-acetic acid for protracted periods (more than 24 h) of time. To resolve these problems, we have adopted the use of a transgenic line of Arabidopsis (Arabidopsis thaliana) where the promoter of an abscission-specific polygalacturonase gene (At2g41850/ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE2) has been fused to a green fluorescent protein reporter. RNA was extracted from green fluorescent protein-tagged cells, released from abscising floral organs, and used to generate a complementary DNA library. This library was used to probe a microarray, and a population of abscission-related transcripts was studied in detail. Seven novel abscission-related genes were identified, four of which encode proteins of unknown function. Reverse transcription-polymerase chain reaction analyses and promoter fusions to the b-glucuronidase reporter gene confirmed the expression of these genes in the abscission zone and revealed other places of expression during seedling development. Three of these genes were studied further by crossing reporter lines to the abscission mutants inflorescence deficient in abscission (ida) and blade-on-petiole1 (bop1)/bop2 and an IDA-overexpressing line. Phenotypic analysis of an At3g14380 transfer DNA insertion line indicates that this gene plays a functional role in floral organ shedding. This strategy has enabled us to uncover new genes involved in abscission, and their possible contribution to the process is discussed.

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Section: Proposed Roles For the Abscission-related Genes Identifiedmentioning

confidence: 99%

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confidence: 99%

A Novel Approach to Dissect the Abscission Process in Arabidopsis

et al. 2012

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“…Pectinesterase catalyzes the de‐esterification of these polymers before abscission, as has been observed in poinsettia (Lee et al ), making pectin accessible to other hydrolytic enzymes, including PGs, for further breakdown. Pectinesterase also has been detected in the AZ of citrus (Ratner et al ), Phaseolus vulgaris (bean), and Coleus blumei (Lamotte et al ; Moline et al ).…”

Section: Cell Separationmentioning

confidence: 99%

Letting Go is Never Easy: Abscission and Receptor‐Like Protein Kinases

Niederhuth

Cho

Seitz

et al. 2013

JIPB

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Abscission is the process by which plants discard organs in response to environmental cues/stressors, or as part of their normal development. Abscission has been studied throughout the history of the plant sciences and in numerous species. Although long studied at the anatomical and physiological levels, abscission has only been elucidated at the molecular and genetic levels within the last two decades, primarily with the use of the model plant Arabidopsis thaliana. This has led to the discovery of numerous genes involved at all steps of abscission, including key pathways involving receptor-like protein kinases (RLKs). This review covers the current knowledge of abscission research, highlighting the role of RLKs.

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“…B,1). Mitochondrial MDH (malic dehydrogenase) is unstable when frozen (Nakanish et al, 1969) and pectin esterase from both coleus and bean was denatured by freezing and thawing (Lamotte et a/., 1969). These were apparently concen trated in the cotyledons and were bound to 2-10% lipid (Ghetie and Buzila, 1964b).…”

Section: B Relation To Enzyme Inactivationmentioning

confidence: 99%

Molecular Basis of Freezing Injury and Tolerance

Levitt

1980

Chilling, Freezing, and High Temperature Stresses

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Pectin Esterase in Relation to Leaf Abscission in Coleus and Phaseolus

Cited by 11 publications

References 20 publications

A Novel Approach to Dissect the Abscission Process in Arabidopsis

A Novel Approach to Dissect the Abscission Process in Arabidopsis

Letting Go is Never Easy: Abscission and Receptor‐Like Protein Kinases

Molecular Basis of Freezing Injury and Tolerance

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