Pectin Dependent Cell Adhesion Restored by a Mutant Microtubule Organizing Membrane Protein

Kohorn, Bruce D.; Dexter-Meldrum, Jacob; Zorensky, Frances D H; Chabout, Salem; Mouille, Grégory; Kohorn, Susan L.

doi:10.3390/plants10040690

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…Additionally, we investigated whether gosamt1 gosamt2 hypocotyls presented cell adhesion phenotypes using ruthenium red dye. Ruthenium red cannot enter into WT hypocotyls, but it can enter and dye hypocotyls of mutants with cell adhesion defects 34 . We observed gosamt1 gosamt2 hypocotyls were stained whereas WT hypocotyls were not ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Discovery of putative Golgi S-Adenosyl methionine transporters reveals the importance of plant cell wall polysaccharide methylation

Temple

Phyo

Yang

et al. 2021

Preprint

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Polysaccharide methylation, especially that of pectin, is a common and important feature of land plant cell walls. Polysaccharide methylation takes place in the Golgi apparatus and therefore relies on the import of S-adenosyl methionine (SAM) from the cytosol into the Golgi. However, to date, no Golgi SAM transporter has been identified in plants. In this work, we studied major facilitator superfamily members in Arabidopsis that we identified as putative Golgi SAM transporters (GoSAMTs). Knock-out of the two most highly expressed GoSAMTs led to a strong reduction in Golgi-synthesised polysaccharide methylation. Furthermore, solid-state NMR experiments revealed that reduced methylation changed cell wall polysaccharide conformations, interactions and mobilities. Notably, the NMR revealed the existence of pectin egg-box structures in intact cell walls, and showed that their formation is enhanced by reduced methyl-esterification. These changes in wall architecture were linked to substantial growth and developmental phenotypes. In particular, anisotropic growth was strongly impaired in the double mutant. The identification of putative transporters that import SAM into the Golgi lumen in plants provides new insights into the paramount importance of polysaccharide methylation for plant cell wall structure and function.

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Section: Resultsmentioning

confidence: 99%

Discovery of putative Golgi S-Adenosyl methionine transporters reveals the importance of plant cell wall polysaccharide methylation

Temple

Phyo

Yang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…These HG-modifying enzymes include pectin methylesterases (PMEs) ( Pelloux et al 2007 ), PME inhibitors (PMEIs) ( Coculo and Lionetti 2022 ), acetyl esterases ( Sénéchal et al 2014 ), and polygalacturonases ( Kim et al 2006 ), which hydrolyze the methyl/acetyl esters and/or the HG backbone, respectively ( Amsbury et al 2016 ; Liu et al 2023 ). Changes in HG methyl esterification and/or HG size are associated with numerous HG functions, including cell:cell adhesion ( Kohorn et al 2021a ), cell elongation/shape/growth ( Daher et al 2018 ; Haas et al 2020 ), and fruit ripening ( Paniagua et al 2014 ).…”

Section: The 5 Major Polymers Of the Plant Cell Wallmentioning

confidence: 99%

The plant cell wall—dynamic, strong, and adaptable—is a natural shapeshifter

Delmer,

Dixon,

Keegstra

et al. 2024

The Plant Cell

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Mythology is replete with good and evil shapeshifters, who, by definition, display great adaptability and assume many different forms—with several even turning themselves into trees. Cell walls certainly fit this definition as they can undergo subtle or dramatic changes in structure, assume many shapes, and perform many functions. In this review, we cover the evolution of knowledge of the structures, biosynthesis, and functions of the 5 major cell wall polymer types that range from deceptively simple to fiendishly complex. Along the way, we recognize some of the colorful historical figures who shaped cell wall research over the past 100 years. The shapeshifter analogy emerges more clearly as we examine the evolving proposals for how cell walls are constructed to allow growth while remaining strong, the complex signaling involved in maintaining cell wall integrity and defense against disease, and the ways cell walls adapt as they progress from birth, through growth to maturation, and in the end, often function long after cell death. We predict the next century of progress will include deciphering cell type–specific wall polymers; regulation at all levels of polymer production, crosslinks, and architecture; and how walls respond to developmental and environmental signals to drive plant success in diverse environments.

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Transcriptomic and metabolomic analyses reveal molecular mechanisms associated with the natural abscission of blue honeysuckle (Lonicera caerulea L.) ripe fruits

Chen

Ren

Bian

et al. 2023

Plant Physiology and Biochemistry

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Pectin Dependent Cell Adhesion Restored by a Mutant Microtubule Organizing Membrane Protein

Cited by 4 publications

References 42 publications

Discovery of putative Golgi S-Adenosyl methionine transporters reveals the importance of plant cell wall polysaccharide methylation

Discovery of putative Golgi S-Adenosyl methionine transporters reveals the importance of plant cell wall polysaccharide methylation

The plant cell wall—dynamic, strong, and adaptable—is a natural shapeshifter

Transcriptomic and metabolomic analyses reveal molecular mechanisms associated with the natural abscission of blue honeysuckle (Lonicera caerulea L.) ripe fruits

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