Pectin coated nanostructured lipid carriers for targeted piperine delivery to hepatocellular carcinoma

Shehata, Eman Mm; Gowayed, Mennatallah A.; El-Ganainy, Samar O.; Sheta, Eman; Elnaggar, Yosra S.R.; Abdallah, Ossama Y.

doi:10.1016/j.ijpharm.2022.121712

Cited by 27 publications

(17 citation statements)

References 84 publications

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“…Prior to analysis, FS-NS dispersion was dried using Aerosil ® 200, as previously described [ 36 ]. Briefly, FS-NS aqueous dispersion was mixed with Aerosil ® 200 in a ratio of 4:1, and then the mixture was allowed to dry overnight in a desiccator.…”

Section: Methodsmentioning

confidence: 99%

Targeted Fisetin-Encapsulated β-Cyclodextrin Nanosponges for Breast Cancer

Aboushanab,

El-Moslemany,

El-Kamel

et al. 2023

Pharmaceutics

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Fisetin (FS) is considered a safer phytomedicine alternative to conventional chemotherapeutics for breast cancer treatment. Despite its surpassing therapeutic potential, its clinical utility is hampered by its low systemic bioavailability. Accordingly, as far as we are aware, this is the first study to develop lactoferrin-coated FS-loaded β-cyclodextrin nanosponges (LF-FS-NS) for targeted FS delivery to breast cancer. NS formation through cross-linking of β-cyclodextrin by diphenyl carbonate was confirmed by FTIR and XRD. The selected LF-FS-NS showed good colloidal properties (size 52.7 ± 7.2 nm, PDI < 0.3, and ζ-potential 24 mV), high loading efficiency (96 ± 0.3%), and sustained drug release of 26 % after 24 h. Morphological examination using SEM revealed the mesoporous spherical structure of the prepared nanosponges with a pore diameter of ~30 nm, which was further confirmed by surface area measurement. Additionally, LF-FS-NS enhanced FS oral and IP bioavailability (2.5- and 3.2-fold, respectively) compared to FS suspension in rats. Antitumor efficacy evaluation in vitro on MDA-MB-231 cells and in vivo on an Ehrlich ascites mouse model demonstrated significantly higher activity and targetability of LF-FS-NS (30 mg/kg) compared to the free drug and uncoated formulation. Consequently, LF-FS-NS could be addressed as a promising formulation for the effective management of breast cancer.

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Section: Methodsmentioning

confidence: 99%

Targeted Fisetin-Encapsulated β-Cyclodextrin Nanosponges for Breast Cancer

Aboushanab,

El-Moslemany,

El-Kamel

et al. 2023

Pharmaceutics

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“…Coumarin-6 was observed through the green channel at an excitation wavelength of 485 nm while Hoechst-nuclear counter stain was observed through the blue channel at 430 nm. Finally, the fluorescence intensity in the obtained images was quantified using Image J software (version 1.45 s) as previously described (Ashour et al., 2021 ; Shehata et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Luteolin-loaded exosomes derived from bone marrow mesenchymal stem cells: a promising therapy for liver fibrosis

et al. 2022

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Liver fibrosis is a global life-threatening disorder with no approved treatment. It leads to serious hepatic complications when progressive, such as cirrhosis and carcinoma. Luteolin (LUT) is a plant flavonoid possessing a promising therapeutic potential in various liver diseases particularly, liver fibrosis. It was reported to have potent anti-inflammatory and antioxidant properties. It also suppresses the proliferation of activated hepatic stellate cells (HSC) and induces their apoptosis. However, its poor aqueous solubility and exposure to metabolism hinder its clinical use. Mesenchymal stem cells (MSCs)-derived exosomes, nano-sized extracellular vesicles, have recently emerged as natural biocompatible drug delivery vehicles permitting efficient drug delivery. Accordingly, the present study aimed for the first time to investigate the potential of bone marrow MSCs-derived exosomes to improve LUTs antifibrotic effectiveness. LUT-loaded exosomes (LUT-Ex) were successfully developed, optimized and subjected to both in vitro and in vivo characterization. The elaborated LUT-Ex presented good colloidal properties (size; 150 nm, PDI; 0.3 and ζ-potential; −28 mV), typical vesicular shape, reasonable drug entrapment efficiency (40%) with sustained drug release over 72 h. Additionally, the cellular uptake study of coumarin-6-loaded exosomes in HEP-G2 revealed a significant enhancement in their uptake by 78.4% versus free coumarin-6 solution ( p ≤ 0.001). Following a single intraperitoneal injection, LUT-Ex revealed a superior antifibrotic activity compared with either LUT-suspension or blank exosomes as evidenced by the results of biochemical and histopathological evaluation. In conclusion, the elaborated LUT-Ex could be addressed as a promising nanocarrier for effective treatment of liver fibrosis.

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“…In this experiment, GMS with a phase transition temperature of 67 • C, and oleic acid with a transition temperature of 8 • C, were utilized as solid and liquid lipid, respectively. KA-NLC was prepared by the melting ultrasonic method [44] according to the following procedure; 0.02 g KA, 0.45 g GMS, and 0.15 g oleic acid were mixed and heated under magnetic stirring as the oil phase; 0.3 g Poloxamer 188 was dissolved and heated in 30 mL ultrapure water as the aqueous phase. The aqueous phase was gently and evenly introduced to the oil phase with continuous stirring to make the primary emulsion once both the oil and aqueous phases had completely dissolved and the temperature had reached 80 • C. Further, the obtained emulsion was dispersed evenly by a JY92-II ultrasonic cell grinder (Ningbo Xinzhi Biological Technology Co., Ltd., Ningbo, China) and then chilled and solidified at −20 • C to acquire KA-NLC.…”

Section: Preparation Of Ka-loaded Nlcmentioning

confidence: 99%

Hyaluronic Acid Modified Nanostructured Lipid Carrier for Targeting Delivery of Kaempferol to NSCLC: Preparation, Optimization, Characterization, and Performance Evaluation In Vitro

Liu

Cui

et al. 2022

Molecules

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Lung cancer seriously threatens the health of human beings, with non-small cell lung cancer (NSCLC) accounting for 80%. Nowadays, the potential position of nano-delivery in treating cancer has been the subject of continuous research. The present research aimed to prepare two molecular weight hyaluronic acid (HA)-modified kaempferol (KA)-loaded nanostructured lipid carriers (HA-KA-NLCs) by the method of melting ultrasonic and electrostatic adsorption, and to assess the antitumor effect of the preparations on A549 cells. The characterization and safety evaluation of the preparations illustrated that they are acceptable for drug delivery for cancer. Subsequently, differential scanning calorimetry (DSC) curve and transmission electron microscopy (TEM) images indicated that the drug was adequately incorporated in the carrier, and the particle appeared as a sphere. Moreover, HA-KA-NLC showed predominant in vitro antitumor effects, inhibiting proliferation, migration, and invasion, promoting apoptosis and increasing cellular uptake of A549 cells. Otherwise, the Western blot assay revealed that preparations could activate epithelial-mesenchymal transition (EMT)-related signaling pathways and modulate the expression of E-cadherin, N-cadherin, and Vimentin in A549 cells. Our present findings demonstrated that HA-KA-NLC could be considered as a secure and effective carrier for targeted tumor delivery and may have potential application prospects in future clinic therapy of NSCLC.

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Pectin coated nanostructured lipid carriers for targeted piperine delivery to hepatocellular carcinoma

Cited by 27 publications

References 84 publications

Targeted Fisetin-Encapsulated β-Cyclodextrin Nanosponges for Breast Cancer

Targeted Fisetin-Encapsulated β-Cyclodextrin Nanosponges for Breast Cancer

Luteolin-loaded exosomes derived from bone marrow mesenchymal stem cells: a promising therapy for liver fibrosis

Hyaluronic Acid Modified Nanostructured Lipid Carrier for Targeting Delivery of Kaempferol to NSCLC: Preparation, Optimization, Characterization, and Performance Evaluation In Vitro

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