Pectin and Neutral Monosaccharides Production during the Simultaneous Hydrothermal Extraction of Waste Biomass from Refining of Sugar—Optimization with the Use of Doehlert Design

Pińkowska, Hanna; Krzywonos, Małgorzata; Wolak, Paweł; Złocińska, Adrianna

doi:10.3390/molecules24030472

Cited by 23 publications

(18 citation statements)

References 33 publications

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“…It has a uniform distribution of experimental points and is a rotatable design with constant variance at equidistant from the central point. The experimental domain can be extended by adding new independent variables or moving the design to a new experimental domain [37]. DD requires a smaller number of experiments while obtaining better precision of coefficient estimates and the number of levels studied for each variable changes: five levels for the first parameter, three for the last and seven for the second in the case of a matrix with three variables [38].…”

Section: Methodsmentioning

confidence: 99%

A New Approach of Extraction of α-Amylase/trypsin Inhibitors from Wheat (Triticum aestivum L.), Based on Optimization Using Plackett–Burman and Box–Behnken Designs

et al. 2019

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Wheat is one of the most consumed foods in the world and unfortunately causes allergic reactions which have important health effects. The α-amylase/trypsin inhibitors (ATIs) have been identified as potentially allergen components of wheat. Due to a lack of data on optimization of ATI extraction, a new wheat ATIs extraction approach combining solvent extraction and selective precipitation is proposed in this work. Two types of wheat cultivars (Triticum aestivum L.), Julius and Ponticus were used and parameters such as solvent type, extraction time, temperature, stirring speed, salt type, salt concentration, buffer pH and centrifugation speed were analyzed using the Plackett-Burman design. Salt concentration, extraction time and pH appeared to have significant effects on the recovery of ATIs (p < 0.01). In both wheat cultivars, Julius and Ponticus, ammonium sulfate substantially reduced protein concentration and inhibition of amylase activity (IAA) compared to sodium chloride. The optimal conditions with desirability levels of 0.94 and 0.91 according to the Doehlert design were: salt concentrations of 1.67 and 1.22 M, extraction times of 53 and 118 min, and pHs of 7.1 and 7.9 for Julius and Ponticus, respectively. The corresponding responses were: protein concentrations of 0.31 and 0.35 mg and IAAs of 91.6 and 83.3%. Electrophoresis and MALDI-TOF/MS analysis showed that the extracted ATIs masses were between 10 and 20 kDa. Based on the initial LC-MS/MS analysis, up to 10 individual ATIs were identified in the extracted proteins under the optimal conditions. The positive implication of the present study lies in the quick assessment of their content in different varieties especially while considering their allergenic potential.

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Section: Methodsmentioning

confidence: 99%

A New Approach of Extraction of α-Amylase/trypsin Inhibitors from Wheat (Triticum aestivum L.), Based on Optimization Using Plackett–Burman and Box–Behnken Designs

et al. 2019

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“…The chemical composition of HMP and the WS fractions were determined by high-pressure liquid chromatography (HPLC). A detailed description of the analyses and analytical methods is published elsewhere [25,30].…”

Section: Analytical Techniques and Measurement Methodologymentioning

confidence: 99%

“…A suspension containing water-soluble products (WS) and a water-insoluble solid post-reaction residue (WI) was obtained as a result of the hydrothermolysis of HMP. A detailed description of the separation of HMP hydrothermolysis products is published elsewhere [23][24][25].…”

Section: Separation Of High Methyl Ester Citrus-apple Pectin Hydrothementioning

confidence: 99%

Production of uronic acids by hydrothermolysis of pectin as a model substance for plant biomass waste

Pińkowska

Krzywonos

Wolak

et al. 2019

Green Processing and Synthesis

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The hydrolysis of high methyl ester citrus-apple pectin as a model substance for plant biomass waste rich in pectin fraction resulting in an uronic acids was performed in a batch reactor using subcritical water. The effects of the reaction temperature and time on the composition of the products contained in the separated liquid fractions were studied. The optimal experimental design methodology was used for modelling and optimizing the yield of uronic acids. In good agreement with experimental results (R2 = 0.986), the model predicts an optimal yield of uronic acids (approx. 77.3 g kg-1 ± 0.7 g kg-1) at a temperature and a time of about 155°C and 36 min. Uronic acids were isolated from reaction mixture using the ion exchange method. Higher temperature and longer holding time resulted in a greater degree of thermal degradation of uronic acids and simultaneously higher yield of losses and gas fractions

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“…a ij expresses the weight of the interaction effect between i and j factors, and a ii is considered to be a curve-shaped parameter. The coefficients were calculated by Nemrod software using the least-squares regression [38,39]. For this purpose, multiple experiments (16) were performed according to the Dohlert matrix.…”

Section: Experimental Designmentioning

confidence: 99%

Phosphate Mine Tailing Recycling in Membrane Filter Manufacturing: Microstructure and Filtration Suitability

Loutou¹,

Misrar

Koudad

et al. 2019

Minerals

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Ceramic membrane filters based on industrial by-products can be considered to be a valorization alternative of phosphate mine tailings, even more so if these ceramic membranes are used in the industrial wastewater treatment due to their good mechanical, chemical, and thermal resistance. The depollution of textile industry rejections with this method has not been studied in detail previously. In this work, ceramic membrane filters have been manufactured from natural clay and phosphate mine tailings (phosphate sludge). Blends of the abovementioned materials with a pore-forming agent (sawdust, up to 20 wt. %) were investigated in the range 900–1100 °C using thermal analysis, X-ray diffraction, scanning electron microscopy, and mercury porosimetry. Ceramic properties were measured as a function of firing temperature and sawdust addition. Filtration tests were carried out on samples with advantageous properties. The results showed that gehlenite together with diopside neoformed from lime decomposed carbonates and breakdown products of clay minerals, while calcium phosphate derived from partial decomposition of fluorapatite. Both quartz and fluorapatite resisted heating. The results of the experimental design showed that the variations of physical properties versus processing factors were well described by the polynomial model. Filtration results are quite interesting, allowing these membranes to be used in industrial effluent treatment.

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Pectin and Neutral Monosaccharides Production during the Simultaneous Hydrothermal Extraction of Waste Biomass from Refining of Sugar—Optimization with the Use of Doehlert Design

Cited by 23 publications

References 33 publications

A New Approach of Extraction of α-Amylase/trypsin Inhibitors from Wheat (Triticum aestivum L.), Based on Optimization Using Plackett–Burman and Box–Behnken Designs

A New Approach of Extraction of α-Amylase/trypsin Inhibitors from Wheat (Triticum aestivum L.), Based on Optimization Using Plackett–Burman and Box–Behnken Designs

Production of uronic acids by hydrothermolysis of pectin as a model substance for plant biomass waste

Phosphate Mine Tailing Recycling in Membrane Filter Manufacturing: Microstructure and Filtration Suitability

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