Pectin and Calcium Distribution in Cell Wall Fractions of Physiological Disorder-Affected Mangosteen Fruits

Poovarodom, Sumitra; Phanchindawan, Naree

doi:10.17660/actahortic.2013.984.44

Cited by 1 publication

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“…The polygalacturonase gene decreases in TFD compared to normal conditions as it acts in the softening process, resulting in degradation or solubilization of the cell wall. Increased pectinesterase expression level in TFD showed that the pectin is bound to the cell walls and pectin-bound calcium is higher than normal [25]. Calcium-pectate forms cross-links between homogalacturonan (HGA) in the middle of the lamella so that the pectin-bound calcium is increased.…”

Section: Discussionmentioning

confidence: 99%

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Comparative transcriptome analysis of translucent flesh disorder in mangosteen (Garcinia mangostana L.) fruits in response to different water regimes

et al. 2019

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Translucent flash disorder (TFD) is one of the important physiological disorders in mangosteen ( Garcinia mangostana L.). TFD has symptoms such as flesh arils that become firm and appear transparent similar to watercore in apple or pear. Information on the changes of gene expression in TFD-affected tissues remain limited, and investigations into the effects of different water regimes still need to be undertaken. Through an RNA sequencing approach using the Ion Proton, 183,274 contigs with length ranging from 173–13,035 bp were constructed by de novo assembly. Functional annotation was analyzed using various public databases such as non-redundant protein NCBI, SwissProt, and Gene Ontology, and KEGG pathway. Our studies compared different water regimes to incidence and differentially expressed genes of TFD-like physiological disorders. From the differentially expressed gene (DEG) between normal air and TFD-affected aril, we identified DEG-related TFD events, which 6228 DEGs in the control condition and 3327 DEGs in under water stress treatment condition remained, and confirmed these with RT-qPCR, including sucrose synthase (SUSY), endoglucanase (GUN), xyloglucan endotransglucosylase/hydrolase (XTH), and polygalacturonase (PG) showed statistically significant. In addition, transcription factors also indicated changes in MYB, NAC and WRKY between tissues and different water regimes.

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Section: Discussionmentioning

confidence: 99%

“…TFD-affected cell walls showed that the cells had loss disintegration between middle lamella and primary cell wall and that they were swollen [24]. Pectin composition, as well as calcium distribution on the pectin, was higher in TFD than normal flesh [25].…”

Section: Introductionmentioning

confidence: 99%