Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects

Ning, Xin; Liu, Ying; Jia, Mengdi; Wang, Qidi; Sun, Ziyan; Li, Ji; Mayo, Kevin H.; Zhou, Yifa; Sun, Lin

doi:10.1016/j.carbpol.2021.117925

Cited by 43 publications

(28 citation statements)

References 38 publications

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“…The radical-scavenging activity of pectins can be ascribed to the presence of hydroxyls and carboxyls in the structure of these polysaccharides [ 14 , 15 ]. Many authors suggest that GalA, as a hydrogen donator, probably plays a key role in the scavenging of free radicals [ 16 , 17 ]. Hence, the HG domain may represent an active structural domain of pectin-mediating antioxidant activity [ 15 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Many authors suggest that GalA, as a hydrogen donator, probably plays a key role in the scavenging of free radicals [ 16 , 17 ]. Hence, the HG domain may represent an active structural domain of pectin-mediating antioxidant activity [ 15 , 17 ]. However, some authors believe that its antioxidant activity is associated with RG-I [ 18 ], and [ 19 ] showed that antioxidant activity decreases with an increase in GalA content.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Popov¹,

Смирнов²,

Kvashninova³

et al. 2021

Molecules

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The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively. EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17–133 μg/mL, whereas commercial apple pectin scavenged at 0.5–2 mg/mL. The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50—0.050, 0.109 and 1.961 mg/mL), respectively. Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively. Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains. The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Popov¹,

Смирнов²,

Kvashninova³

et al. 2021

Molecules

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“…The strong absorption at about 3,370 cm –1 could be assigned to -OH stretching in polysaccharides. A moderate band at around 2,940 cm –1 was attributed to the stretching of -CH groups ( 27 ). The COO- doublet, which was characteristic for pectin at 1,740 and 1,600 cm –1 , could be clearly observed, corresponding to C = O vibration of methyl-esterified carboxyl groups (COO-R) and ionic carboxyl groups (COO-), respectively ( 28 ).…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

et al. 2022

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Tea is a popular beverage with a long history of safe and healthy use. Tea polysaccharide is a bioactive component extracted from tea, which has attracted more and more attention in recent decades. In this article, an acidic polysaccharide Gougunao tea polysaccharide (GPS) was isolated from Gougunao green tea by hot water extraction and ethanol precipitation. After purification by a diethylaminoethyl (DEAE) Sepharose Fast Flow column and a Sephacryl S-400 column, several homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) fractions were obtained. Fraction GPS2b with the highest yield was selected for structural characterization by methylation and nuclear magnetic resonance (NMR) analysis. GPS2b was found to be an HG-type pectic polysaccharide (degree of methyl esterification [DE], 51.6%) with low molecular weight (Mw, 36.8 kDa). It was mainly composed of →4)-α-GalpA- (1→ and →4)-α-GalpA-6-OMe-(1→. In addition, a minor highly branched RG-I domain was identified in this fraction. The investigation of structural features of tea polysaccharides can provide insights to understand their structure-bioactivity relationship.

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“…The total polysaccharide was extracted from dried plants (MP) as previously described ( 34 ) and fractioned using DEAE cellulose ion-exchange chromatography with distilled water and 0.5 M NaCl as eluents to obtain neutral (MPP-N) and acidic polysaccharides (MPP-A). Total pectin extracts were loaded onto a DEAE cellulose column and eluted using a stepwise gradient of aqueous NaCl (0, 0.2, and 0.3 M) to acquire a charge distribution of homogenous fractions.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we have been carrying out a program about comparing pectin structures of plants, which are used as food or herbs in China ( 34 , 35 ). In this study, we reported the results of twenty HG pectins purified from twelve plants first.…”

Section: Introductionmentioning

confidence: 99%

Determining Methyl-Esterification Patterns in Plant-Derived Homogalacturonan Pectins

Cui

Liu

et al. 2022

Front. Nutr.

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Homogalacturonan (HG)-type pectins are nutrient components in plants and are widely used in the food industry. The methyl-esterification pattern is a crucial structural parameter used to assess HG pectins in terms of their nutraceutical activity. To better understand the methyl-esterification pattern of natural HG pectins from different plants, we purified twenty HG pectin-rich fractions from twelve plants and classified them by their monosaccharide composition, Fourier transform-infrared spectroscopy (FT-IR) signatures, and NMR analysis. FT-IR shows that these HG pectins are all minimally esterified, with the degree of methyl-esterification (DM) being 5 to 40%. To examine their methyl-esterification pattern by enzymatic fingerprinting, we hydrolyzed the HG pectins using endo-polygalacturonase. Hydrolyzed oligomers were derivatized with 2-aminobenzamide and subjected to liquid chromatography-fluorescence-tandem mass spectrometry (HILIC-FLR-MSn). Twenty-one types of mono-/oligo-galacturonides having DP values of 1–10 were found to contain nonesterified monomers, dimers, and trimers, as well as oligomers with 1 to 6 methyl-ester groups. In these oligo-galacturonides, MSn analysis demonstrated that the number of methyl-ester groups in the continuous sequence was 2 to 5. Mono- and di-esterified oligomers had higher percentages in total methyl-esterified groups, suggesting that these are a random methyl-esterification pattern in these HG pectins. Our study analyzes the characteristics of the methyl-esterification pattern in naturally occurring plant-derived HG pectins and findings that will be useful for further studying HG structure-function relationships.

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Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects

Cited by 43 publications

References 38 publications

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

Determining Methyl-Esterification Patterns in Plant-Derived Homogalacturonan Pectins

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