Pectate lyase isozymes and the pathogenicity of soft rotting strains of Erwinia for melon and cucumber

The aim of this study was characterized Pectobacterium carotovorum subsp. carotovorum (Pcc) the causal pathogen of watermelon soft rot disease in Iran. Of fifty bacterial isolates with white grey and convex colonies on nutrient agar obtained from symptomatic watermelon, ten isolates were selected for further tests. Pathogenicity tests results showed that all test isolates developed typical water-soak symptoms after 2 days and signs of soft rot began 4 days after inoculation on watermelon fruits. Based on the phenotypic properties, the isolates were identified as Pectobacterium carotovorum subsp. carotovorum. The 16S rDNA sequences of isolates were 99% similar to the corresponding 16S rDNA sequence of the reference Pcc isolate. BOX and ERIC-PCR analysis indicated that genetic diversity was present among the isolated Pcc isolates did not relate to the geographic location isolated from. To the best of our knowledge, this is the first study of biochemical and genotypic characterization of Pcc isolates the causal agents of soft rot disease on watermelon, in Iran.

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“…The same symptoms have been reported on other members of Cucurbitaceae family (El‐Hendawy et al. ; Nazerian et al. ).…”

Section: Discussionsupporting

confidence: 82%

“…El‐Hendawy et al. () found that two strains of Erwinia sp. causing soft rot on melon ( Cucumis melo ) in Egypt have highly pathogenic on melon but non‐pathogenic on the related crop cucumber ( C. Sativus ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Pectobacterium carotovorum subsp. carotovorum Causing Watermelon Soft Rot Disease in Iran

Dana

Khodakaramian

Rouhrazi

2015

Journal of Phytopathology

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“…with soft‐rot disease of carrots and peppers has been reported before (Sherf and Macnab, 1986; El‐Shaieb and Malibari, 1995;Agrios, 1997; Farrar et al, 2000; Hadas et al, 2001). Although certain pathogenic specialization for different hosts and variability in host range and pathogenicity are noticed among soft‐rot Erwinias (Dickey, 1979, 1981; Gabor and Moustafa, 1983; Arsenijevic and Obradovic, 1996; El‐Hendawy et al, 1997), similar results were obtained from pepper and carrot strains. They macerated carrot and potato discs and were found to be pathogenic for cucumber, eggplant and melon seedlings as well as pepper and tomato fruits and seedlings.…”

Section: Discussionmentioning

confidence: 57%

Pectic Enzymes Produced In vitro and In vivo by Erwinia spp. Isolated from Carrot and Pepper in Egypt

El-Hendawy

Osman

Ramadan

2002

Journal of Phytopathology

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Thirty soft-rot Erwinia strains were isolated from diseased carrot (Daucus carrota) roots and pepper (Capsicum annuum) fruits. Pathogenicity tests showed that these strains are able to induce soft-rot disease symptoms in different vegetable species indicating a wide host range. Although pectin methyl esterase and polygalacturonase activities were not detected in culture supernatants, they were found in extracts of carrot roots and pepper fruits infected with three strains representing three soft-rot Erwinias. On the other hand, culture supernatants and extracts of infected plant tissues contained pectic lyase activity. The enzyme from either sodium polypectate or pectin medium degraded the former more actively than the latter indicating pectate rather than pectin lyase activity. In contrast, the enzyme produced in infected tissue showed higher activity on pectin than sodium polypectate. Moreover, the enzyme activity on pectin was not reduced in the absence of Ca 2+ , which might suggest that, in addition to pectate lyases, pectin lyases could be produced in plant tissues by Erwinia species. U. S.

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Water stress in cucumber cotyledons infected withXanthomonas campestris pv.cucurbitae

El-Hendawy

1999

Folia Microbiol

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Pectate lyase isozymes and the pathogenicity of soft rotting strains of Erwinia for melon and cucumber

Cited by 5 publications

References 9 publications

Characterization of Pectobacterium carotovorum subsp. carotovorum Causing Watermelon Soft Rot Disease in Iran

Characterization of Pectobacterium carotovorum subsp. carotovorum Causing Watermelon Soft Rot Disease in Iran

Pectic Enzymes Produced In vitro and In vivo by Erwinia spp. Isolated from Carrot and Pepper in Egypt

Water stress in cucumber cotyledons infected withXanthomonas campestris pv.cucurbitae

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