PEBBLE Nanosensors for Bioanalysis

Monson, Eric; Brasuel, Murphy; Philbert, Martin A.; Kopelman, Raoul

doi:10.1201/9780203008997.ch59

Cited by 30 publications

(35 citation statements)

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“…bovine serum albumin) from diffusing through [27][28][29]. The matrix protected entrapped dyes from the intracellular environment, thus diminishing interference with the fluorescent properties of the dyes [25]. Our findings here are in accordance with the previous results on polyacrylamide and sol-gel silica matrix, demonstrating the shielding effect of the matrix in such a designed NP.…”

Section: Prevention Of Enzymatic Reduction Of Mb By the Matrixsupporting

confidence: 82%

“…Our concept of a nanoparticle platform (NP) was initiated by the design of Photonic Explorers for Biomedical use by Biologically Localized Embedding, termed PEBBLEs. Based on the previously demonstrated advantages of PEBBLEs [25], the development of NPs enables the use of PDT methods that differ significantly from conventional approaches. Rather than releasing photosensitizers, the NP is able to deliver only photo-generated cytotoxic species.…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Tang

Park

et al. 2008

Biochemical and Biophysical Research Communications

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107

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The ability to prevent methylene blue (MB), a photosensitizer, from being reduced by plasma reductases will greatly improve its efficacy in photodynamic therapy (PDT) applications. We have developed a delivery approach for PDT by encapsulating MB using nanoparticle platforms (NPs). The 30-nm polyacrylamide-based NPs provide protection for the embedded MB against reduction by diaphorase enzymes. Furthermore, our data shows the matrix-protected MB efficiently induces photodynamic damage to tumor cells. The unprecedented results demonstrate the significant in vitro photodynamic effectiveness of MB when encapsulated within NPs, which promises to open new opportunities for MB in its in vivo and clinical studies.

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Section: Prevention Of Enzymatic Reduction Of Mb By the Matrixsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Tang

Park

et al. 2008

Biochemical and Biophysical Research Communications

Self Cite

107

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“…Finally, it has to be noted that luminescent dyes used as O2 optical indicators in biological studies can be toxic to the cells or can be altered by the chemical interference with the biological environment. Therefore, their dispersion in the cell medium has to be avoided and supporting biofriendly matrixes have to be developed, suited to carry and/or encapsulate them 33,34 . TiO2 particles are instead self-standing, possibly avoiding the need to provide such a suitable immobilization support.…”

Section: Figmentioning

confidence: 99%

Unconventional ratiometric-enhanced optical sensing of oxygen by mixed-phase TiO2

Lettieri

Pallotti

Gesuele

et al. 2016

Applied Physics Letters

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Abstract. We show that mixed-phase titanium dioxide (TiO2) can be effectively employed as an unconventional, inorganic, dual-emitting and ratiometric optical sensor of O2. Simultaneous availability of rutile and anatase TiO2 PL and their peculiar "anti-correlated" PL responses to O2 allow using their ratio as measurement parameter associated to O2 concentration, leading to an experimental responsivity being by construction larger than the one obtainable for single-phase PL detection. A proof of this concept in given, showing a two-fold enhancement of the optical responsivity provided by the ratiometric approach. Besides the peculiar ratiometric-enhanced responsivity, other characteristics of mixed phase TiO2 can be envisaged as favorable for O2 optical probing, namely: a) low production costs, b) absence of heterogeneous components, c) self-supporting properties. These characteristics encourage experimenting its use for applications requiring high indicator quantities at competitive price, possibly also tackling the need to develop supporting matrixes that carry the luminescent probes and avoiding issues related to the use of different components for ratiometric sensing.Determining the content of molecular oxygen (O2) in gas mixtures and/or aqueous environments is important in many different fields. For example, monitoring the O2 concentration in the exhaust stream of an internal combustion engine provides information on the air-fuel ratio of the combustion process, whose control is relevant for reducing harmful emission [1][2][3] . Knowing the concentration of O2 dissolved in water (also "dissolved oxygen", DO) is also crucial in many applications. DO level is intimately related to the state of health of marine habitats, to the concentration of organic pollutants in water 4 and is of great importance for biological studies. In fact, DO content in cell culture media affects cell growth rate, metabolism and protein synthesis, exerting a decisive influence on the cellular functions and behaviors. To mention a few examples, hypoxia/re-oxygenation induce stem cell like

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“…40 Furthermore, operators develop a high level of skill to ensure the insertion does not cause excessive damage when probes are positioned in delicate structures such as subcellular microenvironments. 41 The point-to-point measurements made by fiber optics probes are unable to fully characterize large volumes in technologies such as bioreactors. This is because bioreactors are continually evolving and developing regions of distinct biochemical heterogeneity for which fiber optic based sensors are unable to characterize, which can be referred to as measurement dark zones.…”

Section: A Fiber Optic Sensorsmentioning

confidence: 99%

Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review)

Harrison

Chauhan

2017

Biointerphases

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Cell and gene therapies (CGTs) are examples of future therapeutics that can be used to cure or alleviate the symptoms of disease, by repairing damaged tissue or reprogramming defective genetic information. However, despite the recent advancements in clinical trial outcomes, the path to wide-scale adoption of CGTs remains challenging, such that the emergence of a “blockbuster” therapy has so far proved elusive. Manufacturing solutions for these therapies require the application of scalable and replicable cell manufacturing techniques, which differ markedly from the existing pharmaceutical incumbent. Attempts to adopt this pharmaceutical model for CGT manufacture have largely proved unsuccessful. The most significant challenges facing CGT manufacturing are process analytical testing and quality control. These procedures would greatly benefit from improved sensory technologies that allow direct measurement of critical quality attributes, such as pH, oxygen, lactate and glucose. In turn, this would make manufacturing more robust, replicable and standardized. In this review, the present-day state and prospects of CGT manufacturing are discussed. In particular, the authors highlight the role of fluorescent optical sensors, focusing on their strengths and weaknesses, for CGT manufacture. The review concludes by discussing how the integration of CGT manufacture and fluorescent optical sensors could augment future bioprocessing approaches.

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PEBBLE Nanosensors for Bioanalysis

Cited by 30 publications

References 0 publications

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Unconventional ratiometric-enhanced optical sensing of oxygen by mixed-phase TiO2

Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review)

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