Pearl Millet Aquaporin Gene PgPIP2;6 Improves Abiotic Stress Tolerance in Transgenic Tobacco

Reddy, Palakolanu Sudhakar; Dhaware, Mahamaya G.; Kaliamoorthy, Sivasakthi; Divya, Kummari; Nagaraju, M.; Cindhuri, Katamreddy Sri; Kishor, P. B. Kavi; Bhatnagar‐Mathur, Pooja; Vadez, Vincent; Sharma, Kiran K.

doi:10.3389/fpls.2022.820996

Cited by 14 publications

(11 citation statements)

References 117 publications

(161 reference statements)

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“…These variations in evaluated pearl millet genotypes could presumably be due to repressive effects of higher ion accumulation and hyper-osmotic stress on root hydraulic conductance and accelerated water loss from the leaf tissues (Dhansu et al, 2021;Sheoran et al, 2021). Earlier studies have also reported efficient water conservation system in pearl millet by means of lowering the leaf transpiration rate and reducing leaf area; hence, improved transpiration efficiency, plant water relations (RWC) and membrane stability under stress conditions (Vijayalakshmi et al, 2012;Reddy et al, 2022;Sheoran et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Deciphering trait associated morpho-physiological responses in pearlmillet hybrids and inbred lines under salt stress

et al. 2023

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Pearl millet is a staple food for more than 90 million people residing in highly vulnerable hot arid and semi–arid regions of Africa and Asia. These regions are more prone to detrimental effects of soil salinity on crop performance in terms of reduced biomass and crop yields. We investigated the physiological mechanisms of salt tolerance to irrigation induced salinity stress (ECiw ~3, 6 & 9 dSm–1) and their confounding effects on plant growth and yield in pearl millet inbred lines and hybrids. On average, nearly 30% reduction in above ground plant biomass was observed at ECiw ~6 dSm-1 which stretched to 56% at ECiw ~9 dSm-1 in comparison to best available water. With increasing salinity stress, the crop performance of test hybrids was better in comparison to inbred lines; exhibiting relatively higher stomatal conductance (gS; 16%), accumulated lower proline (Pro; –12%) and shoot Na+/K+(–31%), synthesized more protein (SP; 2%) and sugars (TSS; 32%) compensating in lower biomass (AGB; –22%) and grain yield (GY: –14%) reductions at highest salinity stress of ECiw ~9 dSm–1. Physiological traits modeling underpinning plant salt tolerance and adaptation mechanism illustrated the key role of 7 traits (AGB, Pro, SS, gS, SPAD, Pn, and SP) in hybrids and 8 traits (AGB, Pro, PH, Na+, K+, Na+/K+, SPAD, and gS) in inbred lines towards anticipated grain yield variations in salinity stressed pearl millet. Most importantly, the AGB alone, explained >91% of yield variation among evaluated hybrids and inbreed lines at ECiw ~9 dSm–1. Cumulatively, the better morpho–physiological adaptation and lesser yield reduction with increasing salinity stress in pearl millet hybrids (HHB 146, HHB 272, and HHB 234) and inbred lines (H77/833–2–202, ICMA 94555 and ICMA 843–22) substantially complemented in increased plant salt tolerance and yield stability over a broad range of salinity stress. The information generated herein will help address in deciphering the trait associated physiological alterations to irrigation induced salt stress, and developing potential hybrids in pearl millet using these parents with special characteristics.

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Section: Discussionmentioning

confidence: 99%

Deciphering trait associated morpho-physiological responses in pearlmillet hybrids and inbred lines under salt stress

et al. 2023

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“…The R package, weighted gene correlation network analysis (WGCNA), was used to construct a gene coexpression network and to find coexpressed genes [ 14 ]. Gene expression data and TPM of 16 plants with two different traits (CC and RWC) were used for WGCNA analysis.…”

Section: Methodsmentioning

confidence: 99%

“…This study also reported photosynthesis, plant hormone signal transduction, and mitogen-activated kinase signaling as a drought-responsive pathway [ 10 ]. The function of several abiotic-stress-responsive genes and small RNAs has been studied in several plants [ 11 ] and pearl millet [ 12 , 13 , 14 , 15 , 16 ]. However, knowledge of molecular mechanisms regulating physiological responses is equally critical and important for ensuring the quality and yield of plants.…”

Section: Introductionmentioning

confidence: 99%

Gene Coexpression Analysis Identifies Genes Associated with Chlorophyll Content and Relative Water Content in Pearl Millet

Shinde

Dudhate

Sathe

et al. 2023

Plants

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Pearl millet is a significant crop that is tolerant to abiotic stresses and is a staple food of arid regions. However, its underlying mechanisms of stress tolerance are not fully understood. Plant survival is regulated by the ability to perceive a stress signal and induce appropriate physiological changes. Here, we screened for genes regulating physiological changes such as chlorophyll content (CC) and relative water content (RWC) in response to abiotic stress by using “weighted gene coexpression network analysis” (WGCNA) and clustering changes in physiological traits, i.e., CC and RWC associated with gene expression. Genes’ correlations with traits were defined in the form of modules, and different color names were used to denote a particular module. Modules are groups of genes with similar patterns of expression, which also tend to be functionally related and co-regulated. In WGCNA, the dark green module (7082 genes) showed a significant positive correlation with CC, and the black (1393 genes) module was negatively correlated with CC and RWC. Analysis of the module positively correlated with CC highlighted ribosome synthesis and plant hormone signaling as the most significant pathways. Potassium transporter 8 and monothiol glutaredoxin were reported as the topmost hub genes in the dark green module. In Clust analysis, 2987 genes were found to display a correlation with increasing CC and RWC. Furthermore, the pathway analysis of these clusters identified the ribosome and thermogenesis as positive regulators of RWC and CC, respectively. Our study provides novel insights into the molecular mechanisms regulating CC and RWC in pearl millet.

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“…A pearl millet aquaporin gene transferred to tobacco conferred greater drought, heat, and higher water use efficiency (Reddy et al, 2022). Knockout and overexpression mutants showed a specific AQP isoform in maize roots was an important regulator of root hydraulic conductance, with effects on plant growth (Ding et al, 2020).…”

Section: Root Conductancementioning

confidence: 99%

Optimizing Crop Water Use for Drought and Climate Change Adaptation Requires a Multi-Scale Approach

2022

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Selection criteria that co-optimize water use efficiency and yield are needed to promote plant productivity in increasingly challenging and variable drought scenarios, particularly dryland cereals in the semi-arid tropics. Optimizing water use efficiency and yield fundamentally involves transpiration dynamics, where restriction of maximum transpiration rate helps to avoid early crop failure, while maximizing grain filling. Transpiration restriction can be regulated by multiple mechanisms and involves cross-organ coordination. This coordination involves complex feedbacks and feedforwards over time scales ranging from minutes to weeks, and from spatial scales ranging from cell membrane to crop canopy. Aquaporins have direct effect but various compensation and coordination pathways involve phenology, relative root and shoot growth, shoot architecture, root length distribution profile, as well as other architectural and anatomical aspects of plant form and function. We propose gravimetric phenotyping as an integrative, cross-scale solution to understand the dynamic, interwoven, and context-dependent coordination of transpiration regulation. The most fruitful breeding strategy is likely to be that which maintains focus on the phene of interest, namely, daily and season level transpiration dynamics. This direct selection approach is more precise than yield-based selection but sufficiently integrative to capture attenuating and complementary factors.

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Pearl Millet Aquaporin Gene PgPIP2;6 Improves Abiotic Stress Tolerance in Transgenic Tobacco

Cited by 14 publications

References 117 publications

Deciphering trait associated morpho-physiological responses in pearlmillet hybrids and inbred lines under salt stress

Deciphering trait associated morpho-physiological responses in pearlmillet hybrids and inbred lines under salt stress

Gene Coexpression Analysis Identifies Genes Associated with Chlorophyll Content and Relative Water Content in Pearl Millet

Optimizing Crop Water Use for Drought and Climate Change Adaptation Requires a Multi-Scale Approach

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