Peanut genes identified during initial phase of Cercosporidium personatum infection

Nóbile, Paula Macedo; Lopes, Catalina Romero; Barsalobres-Cavallari, Carla; Quecini, Vera; Coutinho, Luiz Lehmann; Hoshino, Andrea Akemi; Gimenes, Marcos Aparecido

doi:10.1016/j.plantsci.2007.09.009

Cited by 18 publications

(22 citation statements)

References 39 publications

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“…The gene with the least stable expression was 14-3-3 (stability 5 1.11; M value 5 0.124), followed by ubq10 (according to NormFinder, stability 5 0.865) and gapdh (according to GeNorm, M value 5 0.115). Interestingly, the ubq10 (ubiquitin) gene, which has been used in several qPCR studies in Arachis (Luo et al, 2005;Nobile et al, 2007), showed quite a low level of stability in this study.…”

Section: Ranking the Candidate Reference Genesmentioning

confidence: 58%

“…Remans et al, 2008). In Arachis, for example, the ubiquitin gene is sometimes used in qPCR studies as a stable housekeeping gene (e.g., Luo et al, 2005;Nobile et al, 2007). However, several reports have shown that the expression levels of these so-called housekeeping genes differ among different plant tissues and organs (Jain et al, 2006;Remans et al, 2008;Barsalobres-Cavallari et al, 2009).…”

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confidence: 99%

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Identification of Suitable Internal Control Genes for Quantitative Real-Time PCR Expression Analyses in Peanut (Arachis hypogaea)

Brand¹,

Hovav²

2010

Peanut Science

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Real-time qPCR is currently the most sensitive technique available for the detection of lowlevel mRNA expression. For more reliable and precise gene expression analyses, real-time PCR data for a sequence of interest must be normalized against that of a control gene, which is uniformly expressed in various tissues and during different phases of development. So far, suitable internal controls for gene expression studies in peanut have not been identified. We assessed the expression of 10 frequently used housekeeping genes, specifically ubq10, gapdh, hel1, yls8, 14-3-3, 60s, ubc, ef-1a, act7, and adh3. Using the algorithms available through the GeNorm and NormFinder programs, the stability of their expression was estimated in a set of five diverse peanut tissue samples derived from a Virginiatype peanut cultivar (Shulamit). Collectively, the gene with the most stable expression across all of the examined tissues and both programs was adh3, followed by 60s and yls8, which had minimal estimated intra-and inter-tissue variation. The stability of two stable reference genes (adh3 and yls8) compared with two less stable (14-3-3 and ubq10) reference genes was validated in unpooled tissue samples from five peanut kernel developmental stages. Finally, the effect of the use of one or more reference genes on the observed relative expression levels of an important seed oil metabolism gene, diacylglycerol acyltransferase 1 (Dgat1), during kernel development was demonstrated. Based on findings, the suggestion is that adh3, or a combination of this gene with 60s and yls8 should be considered for use in quantitative mRNA expression analyses in Arachis, particularly in studies involving seed development; whereas ubq10 and gapdh should be avoided.

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Section: Ranking the Candidate Reference Genesmentioning

confidence: 58%

mentioning

confidence: 99%

Identification of Suitable Internal Control Genes for Quantitative Real-Time PCR Expression Analyses in Peanut (Arachis hypogaea)

Brand¹,

Hovav²

2010

Peanut Science

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“…Though LLS disease on peanut is of great economic importance as it alone can reduce crop yields drastically, the interaction has not been studied at molecular level for a long time. There were only two reports on the identification of genes involved in peanut (A. hypogaea)-P. personata interaction (Luo et al 2005;Nobile et al 2008). Though the wild relatives of Arachis are highly resistant to several pathogens including P. personata (Rao et al 2003), attempts aimed at using them were confined to breeding programs for a long time.…”

Section: Isolation Of Early Response Genes and Role Of Genes Involvedmentioning

confidence: 99%

Differential gene expression in Arachis diogoi upon interaction with peanut late leaf spot pathogen, Phaeoisariopsis personata and characterization of a pathogen induced cyclophilin

Kumar

Kirti

2011

Plant Mol Biol

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The wild relatives of peanut are resistant to various economically important diseases including late leaf spot (LLS) caused by Phaeoisariopsis personata, compared with the susceptible cultivated peanut (Arachis hypogaea L.). The interaction of the late leaf spot pathogen, Phaeoisariopsis personata and the highly resistant, diploid peanut wild species, Arachis diogoi was analyzed at the molecular level by differential gene expression studies. Genes up-regulated with in 48 h of pathogen challenge were isolated as partial cDNAs. Some of the isolated genes, which are shown to be involved in the first line of defense in plants, were further characterized with respect to their transcriptional regulation in response to pathogen. Among the isolated clones, two were found to encode cyclophilin like proteins. One of the two isolated partial cDNAs encoding cyclophilin like proteins was extended using 5' RACE. The full length cDNA, designated as AdCyp, was 886 bp in length and encodes a polypeptide of 172 amino acids. Southern hybridization suggests that AdCyp is possibly coded by a single gene and at least one more identical gene is present in Arachis diogoi genome. AdCyp exhibits evolutionary conservation across the kingdoms. Phylogenetic analysis showed that AdCyp belongs to the subgroup I of Group I in cyclophilins. A translational fusion of GFP-AdCyp was found to localize to both cytosol and nucleus. AdCyp transcripts were found to accumulate in response to the treatments with pathogen as well as phytohormones. Constitutive heterologous expression of AdCyp resulted in enhanced resistance to Ralstonia solanacearum and reduced susceptibility towards Phytophthora parasitica var. nicotianae in transgenic tobacco and the resistance was associated with higher transcript levels of various defense related genes.

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“…Homologies were detected using Blastx, E-values <1e-10. Eight are homologs of Arabidopsis-NBS-encoding genes, one is a homolog to a dehydration-responsive element, and another is a homolog of glutathione S-transferase (Proite et al, 2007;Nobile et al, 2008).…”

Section: Selection Of Sequences and Primer Designmentioning

confidence: 99%

Development and use of single nucleotide polymorphism markers for candidate resistance genes in wild peanuts (Arachis spp)

Alves¹,

Pereira²,

Leal‐Bertioli³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. The cultivated peanut (Arachis hypogaea L.) is an allotetraploid of recent origin, with an AABB genome and low genetic diversity. Perhaps because of its limited genetic diversity, this species lacks resistance to a number of important pests and diseases. In contrast, wild species of Arachis are genetically diverse and are rich sources of disease resistance genes. Consequently, a study of wild peanut relatives is attractive from two points of view: to help understand peanut genetics and to characterize wild alleles that could confer disease resistance. With this in mind, a diploid population from a cross between two wild peanut relatives was developed, in order to make a dense genetic map that could serve as a reference for peanut genetics and in order to characterize the regions of the Arachis genome that code for disease resistance. We tested two methods for developing and genotyping single nucleotide polymorphisms in candidate genes for disease resistance; one is based on single-base primer extension methods and the other is based on amplification refractory mutation system-polymerase chain reaction. We found single-base pair extension to be an efficient method, suitable for high-throughput, single-nucleotide polymorphism mapping; it allowed us to locate five candidate genes for resistance on our genetic map.

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Peanut genes identified during initial phase of Cercosporidium personatum infection

Cited by 18 publications

References 39 publications

Identification of Suitable Internal Control Genes for Quantitative Real-Time PCR Expression Analyses in Peanut (Arachis hypogaea)

Identification of Suitable Internal Control Genes for Quantitative Real-Time PCR Expression Analyses in Peanut (Arachis hypogaea)

Differential gene expression in Arachis diogoi upon interaction with peanut late leaf spot pathogen, Phaeoisariopsis personata and characterization of a pathogen induced cyclophilin

Development and use of single nucleotide polymorphism markers for candidate resistance genes in wild peanuts (Arachis spp)

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