Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticusinfection

Guo, Bao‐Zhu; Chen, Xiaoping; Dang, Phat; Scully, Brian T.; Liang, Xuanqiang; Holbrook, C. C.; Yu, Jiujiang; Culbreath, A. K.

doi:10.1186/1471-213x-8-12

Cited by 96 publications

(85 citation statements)

References 56 publications

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“…Two submissions were 7454 and 2184 sequences from seed libraries of the Chinese accessions Luhua 14 and Shanyou 523, respectively. The majority represented the 21,777 ESTs (accession numbers ES702769 to ES724546) from developing seeds (Guo et al, 2008a) of the U.S. runner type cultivar Tifrunner and the Spanish breeding line GT-C20 (Guo et al, 2008b). The seed and leaf EST originated from 10 non-normalized peanut cDNA libraries (Guo et al, 2007b;Guo et al, 2008a), resulting in over 13,824 unigene ESTs, which will be used for the production of a long-oligo microarray for peanut.…”

Section: Functional Genomics and Control Strategies Of Aflatoxin Contmentioning

confidence: 99%

“…The majority represented the 21,777 ESTs (accession numbers ES702769 to ES724546) from developing seeds (Guo et al, 2008a) of the U.S. runner type cultivar Tifrunner and the Spanish breeding line GT-C20 (Guo et al, 2008b). The seed and leaf EST originated from 10 non-normalized peanut cDNA libraries (Guo et al, 2007b;Guo et al, 2008a), resulting in over 13,824 unigene ESTs, which will be used for the production of a long-oligo microarray for peanut. This sequence data has been made available to the community in order to develop genomic tools and resources for deciphering the biological function of genes in the peanut genome, including a) construction of peanut 70-mer oligo microarray, and b) development of markers/genes associated with resistance to biotic and abiotic stress.…”

Section: Functional Genomics and Control Strategies Of Aflatoxin Contmentioning

confidence: 99%

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Strategies in Prevention of Preharvest Aflatoxin Contamination in Peanuts: Aflatoxin Biosynthesis, Genetics and Genomics

Guo¹,

Yu²,

Holbrook³

et al. 2009

Peanut Science

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Peanut (Arachis hypogaea L.), or groundnut, is an important crop economically and nutritionally in many tropical and subtropical areas of the world. It is also one of the most susceptible host crops to Aspergillus flavus resulting in aflatoxin contamination. The prevention or elimination of aflatoxin contamination in preharvest and postharvest crops is a serious challenge facing scientists. The recent International Conference on Groundnut Aflatoxin Management and Genomics held in Guangzhou, China, provided an international forum for discussions on the latest accomplishments, the development of strategies, and the initiation of cooperative research for the prevention of aflatoxin contamination. This review summarizes the progress in genetic and genomic research of peanuts and the toxinproducing fungus A. flavus. In particular, the pathway for production and the genetic regulation of afaltoxin, and the peanut-Aspergillus interaction are discussed. The use of a peanut-Aspergillus microarray will help scientists to study the croppathogen interaction; aids in the identification of genes involved in both fungal invasion and crop resistance, and ultimately enhance research to find solutions that prevent aflatoxin contamination in agricultural commodities.

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Section: Functional Genomics and Control Strategies Of Aflatoxin Contmentioning

confidence: 99%

Section: Functional Genomics and Control Strategies Of Aflatoxin Contmentioning

confidence: 99%

Strategies in Prevention of Preharvest Aflatoxin Contamination in Peanuts: Aflatoxin Biosynthesis, Genetics and Genomics

Guo¹,

Yu²,

Holbrook³

et al. 2009

Peanut Science

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“…Advances in other marker types, such as randomly amplified polymorphic DNA (RAPD) (Halward and Stalker, 1991;Halward et al, 1992), restriction fragment length polymorphism (RFLP) (Halward and Stalker, 1991;Kochert et al, 1996;Burow et al, 2009), amplified fragment length polymorphism (AFLP) (Herselman et al, 2004), simple sequence repeat (SSR) (He et al, 2003;Gimenes et al, 2007;Cuc et al, 2008;Liang et al, 2009), sequence-related amplified polymorphism (SRAP) , single strand conformational polymorphism (SSCP) (Nagy et al, 2010), and single nucleotide polymorphism (SNP) (Nagy et al, 2012), soon replaced the early exploration with proteins. During the past two decades, much effort has been made to develop genetic and genomic tools in cultivated peanut, such as construction of BAC libraries (Yuksel and Paterson, 2005;Guimarães et al, 2008), cDNA libraries (Luo et al, 2005;Proite et al, 2007;Guo et al, 2008Guo et al, , 2009Koilkonda et al, 2012), RNAseq using next generation sequencing technology (Guimaraes et al, 2012;Zhang et al, 2012) and development of DNA markers (see reviews of Feng et al, 2012;Pandey et al, 2012;Zhao et al, 2012; (Table 1). Among the various molecular markers investigated to date, simple sequence repeats (SSR) have emerged as one of the preferred DNA marker system for conducting genetic and genomic studies in cultivated peanut.…”

Section: Recent Development In Molecular Markersmentioning

confidence: 99%

Recent Advances in Molecular Genetic Linkage Maps of Cultivated Peanut

et al. 2013

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The competitiveness of peanuts in domestic and global markets has been threatened by losses in productivity and quality that are attributed to diseases, pests, environmental stresses and allergy or food safety issues. Narrow genetic diversity and a deficiency of polymorphic DNA markers severely hindered construction of dense genetic maps and quantitative trait loci (QTL) mapping in order to deploy linked markers in marker-assisted peanut improvement. The U.S. Peanut Genome Initiative (PGI) was launched in 2004, and expanded to a global effort in 2006 to address these issues through coordination of international efforts in genome research beginning with molecular marker development and improvement of map resolution and coverage. Ultimately, a peanut genome sequencing project was launched in 2012 by the Peanut Genome Consortium (PGC). We reviewed the progress for accelerated development of peanut genomic resources in peanut, such as generation of expressed sequenced tags (ESTs) (252,832 ESTs as December 2012 in the public NCBI EST database), development of molecular markers (over 15,518 SSRs), and construction of peanut genetic linkage maps, in particular for cultivated peanut. Several consensus genetic maps have been constructed, and there are examples of recent international efforts to develop high density maps. An international reference consensus genetic map was developed recently with 897 marker loci based on 11 published mapping populations. Furthermore, a high-density integrated consensus map of cultivated peanut and wild diploid relatives also has been developed, which was enriched further with 3693 marker loci on a single map by adding information from five new genetic mapping populations to the published reference consensus map.Key Words: Arachis hypogaea, Arachis species, genomics, genetic maps, molecular markers, molecular breeding, Peanut Genome Consortium (PGC).The world's population is predicted to reach nine billion by 2050 (Nature Editorial, 2010), which means greater demand for food, hence, a continuing need to produce improved cultivars of crop plants. Advances in food production will require greater efforts in agricultural research to increase crop yield with improved genetics for plant protection from biotic and abiotic stresses. Agricultural biotechnology is one tool that holds great promise for sustainability of agricultural production and feeding an ever increasing population.Peanut (Arachis hypogaea L.), or groundnut, is one of the major economically important legumes that is cultivated worldwide for its ability to grow in semi-arid environments with relatively low inputs of chemical fertilizers. On a global basis, peanut also is a major source of protein and vegetable oil for human nutrition, containing about 28% protein, 50% oil and 18% carbohydrates. Peanut is cultivated in more than 100 countries in Asia, Africa and the Americas, grown mostly by resource-limited farmers of the semi-arid regions. India and China together produce almost twothirds of the world's peanuts, and the U...

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“…Excitingly, studies on hostresistance mechanisms had make great progress during the past twenty years. With the application of proteomics and genomics technology to high-throughput gene identification, many resistance-associated proteins and genes have been identified in maize [4][5][6] and peanut [7][8][9]. These proteins and genes comprise seed storage proteins, antifungal proteins, pathogenesis-related proteins and stress-related proteins [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…ZmPR10.1 showed a strong inhibition against A. flavus in vitro. Interestingly, in our earlier research, a PR10 up-regulated unique EST sequences was observed only in resistant genotypes "GT-C20" libraries after comparing the gene expression profiling in developing seeds at different reproduction stages during A. parasiticus infection between "Tifrunner", susceptible to A. parasiticus and "GT-C20" [8]. Xie et al [20] was isolated and characterized this up-regulated PR10 (designated as ARAh-PR10, No.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds

Xie¹,

Wen²,

Liu³

et al. 2013

AJPS

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Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype "GT-C20" and susceptible genotype "Yueyou 7". Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.

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Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticusinfection

Cited by 96 publications

References 56 publications

Strategies in Prevention of Preharvest Aflatoxin Contamination in Peanuts: Aflatoxin Biosynthesis, Genetics and Genomics

Strategies in Prevention of Preharvest Aflatoxin Contamination in Peanuts: Aflatoxin Biosynthesis, Genetics and Genomics

Recent Advances in Molecular Genetic Linkage Maps of Cultivated Peanut

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds

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Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticusinfection

Cited by 96 publications

References 56 publications

Strategies in Prevention of Preharvest Aflatoxin Contamination in Peanuts: Aflatoxin Biosynthesis, Genetics and Genomics

Strategies in Prevention of Preharvest Aflatoxin Contamination in Peanuts: Aflatoxin Biosynthesis, Genetics and Genomics

Recent Advances in Molecular Genetic Linkage Maps of Cultivated Peanut

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of &lt;i&gt;Aspergillus flavus&lt;/i&gt; Infection in Peanut Seeds

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Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds