Purpose
Whole-body fat oxidation during exercise can be measured non-invasively during athlete profiling. Gaps in understanding exist in the relationships between fat oxidation during incremental fasted exercise and skeletal muscle parameters, endurance performance, and fat oxidation during prolonged fed-state exercise.
Methods
Seventeen endurance-trained males underwent a (i) fasted, incremental cycling test to assess peak whole-body fat oxidation (PFO), (ii) resting
vastus lateralis
microbiopsy, and (iii) 30-min maximal-effort cycling time-trial preceded by 2-h of fed-state moderate-intensity cycling to assess endurance performance and fed-state metabolism on separate occasions within one week.
Results
PFO (0.58 ± 0.28 g
.
min
−1
) was associated with
vastus lateralis
citrate synthase activity (69.2 ± 26.0 μmol
.
min
−1.
g
−1
muscle protein,
r
= 0.84, 95% CI 0.58, 0.95,
P
< 0.001), CD36 abundance (16.8 ± 12.6 μg
.
g
−1
muscle protein,
r
s
= 0.68, 95% CI 0.31, 1.10,
P
= 0.01), pre-loaded 30-min time-trial performance (251 ± 51 W,
r
= 0.76, 95% CI 0.40, 0.91,
P
= 0.001; 3.2 ± 0.6 W
.
kg
−1
,
r
= 0.62, 95% CI 0.16, 0.86,
P
= 0.01), and fat oxidation during prolonged fed-state cycling (
r
= 0.83, 95% CI 0.57, 0.94,
P
< 0.001). Addition of PFO to a traditional model of endurance (peak oxygen uptake, power at 4 mmol
.
L
−1
blood lactate concentration, and gross efficiency) explained an additional ~ 2.6% of variation in 30-min time-trial performance (adjusted
R
2
= 0.903 vs. 0.877).
Conclusion
These associations suggest non-invasive measures of whole-body fat oxidation during exercise may be useful in the physiological profiling of endurance athletes.