Peak capacity of ion mobility mass spectrometry:

Ruotolo, Brandon T.; Gillig, Kent J.; Stone, Earle G.; Russell, David H.

doi:10.1016/s1570-0232(02)00566-4

Cited by 83 publications

(49 citation statements)

References 26 publications

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“…The incorporation of an IMS drift cell between the LC separation and MS 38, 39 has multiple advantages for analysing complex mixtures such as high speed measurements that allow multiple IMS–MS analyses across a given LC peak, a degree of orthogonality to both LC and MS analyses thereby increasing the overall peak capacity 40, 41 , and a highly reproducible drift time dimension.…”

Section: Hyphenated Methodsmentioning

confidence: 99%

Review on Ion Mobility Spectrometry. Part 2: hyphenated methods and effects of experimental parameters

Cumeras

Figueras

Davis

et al. 2015

Analyst

143

114

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Ion Mobility Spectrometry (IMS) is a widely used and ‘well-known’ technique of ion separation in gaseous phase based on the differences of ion mobilities under an electric field. This technique has received increased interest over the last several decades as evidenced by the pace and advances of new IMS devices available. In this review we explore the hyphenated techniques that are used with IMS, especially mass spectrometry as identification approach and multi-capillary column as pre-separation approach. Also, we will pay special attention to the key figures of merit of the ion mobility spectrum and how data is treated, and the influences of the experimental parameters in both a conventional drift time IMS (DTIMS) and a miniaturized IMS also known as high Field Asymmetric IMS (FAIMS) in the planar configuration. The current review article is preceded by a companion review article which details the current instrumentation and to the sections that configures both a conventional DTIMS and FAIMS devices. Those reviews will give the reader an insightful view of the main characteristics and aspects of the IMS technique.

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Section: Hyphenated Methodsmentioning

confidence: 99%

Review on Ion Mobility Spectrometry. Part 2: hyphenated methods and effects of experimental parameters

Cumeras

Figueras

Davis

et al. 2015

Analyst

143

114

View full text Add to dashboard Cite

show abstract

“…6 By coupling IMS to time-of-flight (TOF) MS, ions can be separated quickly based on both size and mass-to-charge ratios ( m/z ) due to the high speed of IMS and TOF MS analyses. The incorporation of an IMS drift cell between the LC separation and TOF MS has multiple advantages for analyzing complex mixtures such as high speed measurements that allow multiple IMS-MS analyses across a given LC peak, a degree of orthogonality to both LC and MS analyses thereby increasing the overall peak capacity, 7,8 and a highly reproducible drift time dimension. IMS is also able to reduce the number of ions simultaneously arriving at the MS detector, which reduces individual spectral complexity.…”

Section: Introductionmentioning

confidence: 99%

Increasing confidence of LC–MS identifications by utilizing ion mobility spectrometry

Crowell¹,

Baker²,

Payne³

et al. 2013

International Journal of Mass Spectrometry

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Ion mobility spectrometry in conjunction with liquid chromatography separations and mass spectrometry offers a range of new possibilities for analyzing complex biological samples. To fully utilize the information obtained from these three measurement dimensions, informatics tools based on the accurate mass and time tag methodology were modified to incorporate ion mobility spectrometry drift times for peptides observed in human serum. In this work a reference human serum database was created for 12,139 peptides and populated with the monoisotopic mass, liquid chromatography normalized elution time, and ion mobility spectrometry drift time(s) for each. We demonstrate that the use of three dimensions for peak matching during the peptide identification process resulted in an increased numbers of identifications and a lower false discovery rate relative to only using the mass and normalized elution time dimensions.

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“…Secondly, IM-MS is inherently gas-phase and the timescales of separation are well suited for further integration with separations such as liquid chromatography (LC) to further increase peak capacity. 17,18,26-28 Thirdly, different classes of biomolecules are readily distinguished from one another on the basis of their gas-phase structure. This provides higher signal-to-noise for low abundant species of one class ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Biomolecular Signatures of Diabetic Wound Healing by Structural Mass Spectrometry

et al. 2013

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Wound fluid is a complex biological sample containing byproducts associated with the wound repair process. Contemporary techniques, such as immunoblotting and enzyme immunoassays, require extensive sample manipulation and do not permit the simultaneous analysis of multiple classes of biomolecular species. Structural mass spectrometry, implemented as ion mobility-mass spectrometry (IM-MS), comprises two sequential, gas-phase dispersion techniques well suited for the study of complex biological samples due to its ability to separate and simultaneously analyze multiple classes of biomolecules. As a model of diabetic wound healing, polyvinyl alcohol (PVA) sponges were inserted subcutaneously into non-diabetic (control) and streptozotocin-induced diabetic rats to elicit a granulation tissue response and to collect acute wound fluid. Sponges were harvested at days 2 or 5 to capture different stages of the early wound healing process. Utilizing IM-MS, statistical analysis, and targeted ultra-performance liquid chromatography (UPLC) analysis, biomolecular signatures of diabetic wound healing have been identified. The protein S100-A8 was highly enriched in the wound fluids collected from day 2 diabetic rats. Lysophosphatidylcholine (20:4) and cholic acid also contributed significantly to the differences between diabetic and control groups. This report provides a generalized workflow for wound fluid analysis demonstrated with a diabetic rat model.

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Peak capacity of ion mobility mass spectrometry:

Cited by 83 publications

References 26 publications

Review on Ion Mobility Spectrometry. Part 2: hyphenated methods and effects of experimental parameters

Review on Ion Mobility Spectrometry. Part 2: hyphenated methods and effects of experimental parameters

Increasing confidence of LC–MS identifications by utilizing ion mobility spectrometry

Biomolecular Signatures of Diabetic Wound Healing by Structural Mass Spectrometry

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