Peak capacity estimation in isocratic elution

Pous-Torres, S.; Baeza-Baeza, J.J.; Torres-Lapası́o, J.R.; García-Álvarez-Coque, M.C.

doi:10.1016/j.chroma.2008.07.088

Cited by 12 publications

(5 citation statements)

References 26 publications

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“…To assess the column performance, the intra‐column contribution to the peak broadening should be isolated. A simple approach to obtain this information (and additionally the peak asymmetry contribution) is the construction of peak half‐width plots 29. These plots represent the left ( A ) and right ( B ) half‐widths of chromatographic peaks, measured at 10% peak height versus the retention time where m A and m B are the slopes of the linear correlations, and A 0 and B 0 the intercepts (which represent mainly the extra‐column contribution to the peak broadening).…”

Section: Resultsmentioning

confidence: 99%

Comparison of the performance of non‐ionic and anionic surfactants as mobile phase additives in the RPLC analysis of basic drugs

Ruiz-Ángel¹,

García-Álvarez-Coque²

2011

J of Separation Science

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Surfactants added to the mobile phases in reversed-phase liquid chromatography (RPLC) give rise to a modified stationary phase, due to the adsorption of surfactant monomers. Depending on the surfactant nature (ionic or non-ionic), the coated stationary phase can exhibit a positive net charge, or just change its polarity remaining neutral. Also, micelles in the mobile phase introduce new sites for solute interaction. This affects the chromatographic behavior, especially in the case of basic compounds. Two surfactants of different nature, the non-ionic Brij-35 and the anionic sodium dodecyl sulfate (SDS) added to water or aqueous-organic mixtures, are here compared in the separation of basic compounds (β-blockers and tricyclic antidepressants). The reversible/irreversible adsorption of the monomers of both surfactants on the stationary phase was examined. The changes in the nature of the chromatographic system using different columns and chromatographic conditions were followed based on the changes in retention and peak shape. The study revealed that Brij-35 is suitable for analyzing basic compounds of intermediate polarity, using "green chemistry", since the addition of an organic solvent is not needed and Brij-35 is a biodegradable surfactant. In contrast, RPLC with hydro-organic mixtures or mobile phases containing SDS required high concentrations of organic solvents.

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Section: Resultsmentioning

confidence: 99%

Comparison of the performance of non‐ionic and anionic surfactants as mobile phase additives in the RPLC analysis of basic drugs

Ruiz-Ángel¹,

García-Álvarez-Coque²

2011

J of Separation Science

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“…Recently, the peak capacity ( P C ) has been proposed as an alternative to evaluate the real performance of the analytical column 39. This parameter gives the maximal number of resolved peaks that fit in a chromatographic window comprised between t R1 and t R n .…”

Section: Resultsmentioning

confidence: 99%

“…This parameter gives the maximal number of resolved peaks that fit in a chromatographic window comprised between t R1 and t R n . P C can be obtained, using the following expression 39: …”

Section: Resultsmentioning

confidence: 99%

Study of elution behaviour with gradient voltage in CEC using methacrylate monolithic columns

et al. 2010

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A theoretical study on the retention behaviour and chromatographic performance of neutral solutes using a lauryl methacrylate-based monolithic column under voltage gradient mode in CEC was carried out. Through a flexible mathematical function based on a modified Gaussian model, the peak shape of compounds was firstly fitted under constant and gradient voltage. Using the peak shape parameters and retention time, the estimation of global chromatographic performance, efficiency and peak capacity under several voltage conditions was performed. The influence of voltage gradient on the separation efficiency is discussed and simple equations are presented to calculate retention and peak widths under voltage gradient conditions. A comparison in terms of chromatographic performance of a test mixture of neutral solutes under constant and gradient voltage modes was also carried out. The experiments carried out under gradient voltage showed better efficiencies (172,000 plates/m) and lower peak widths than those obtained under constant voltage (52,000 plates/m).

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“…The gradient run induced a peak focusing effect coinciding with lower retention time, resulting in sharper peak shapes and less tailing. To measure this effect quantifiably, the average peak capacity was measured of the isothermal runs and the gradient run as described in literature [45][46][47]. A peak capacity of 55 was obtained for the gradient run (time interval of 20 min), while the peak capacities of the isothermal runs at 25, 40 and 60°C were 50, 54 and 38, respectively.…”

Section: Resultsmentioning

confidence: 99%

Investigation of poly(styrene-divinylbenzene-vinylsulfonic acid) as retentive and electroosmotic flow generating phase in open-tubular electrochromatography

Smet

Lynen

2015

Journal of Chromatography A

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Peak capacity estimation in isocratic elution

Cited by 12 publications

References 26 publications

Comparison of the performance of non‐ionic and anionic surfactants as mobile phase additives in the RPLC analysis of basic drugs

Comparison of the performance of non‐ionic and anionic surfactants as mobile phase additives in the RPLC analysis of basic drugs

Study of elution behaviour with gradient voltage in CEC using methacrylate monolithic columns

Investigation of poly(styrene-divinylbenzene-vinylsulfonic acid) as retentive and electroosmotic flow generating phase in open-tubular electrochromatography

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