Peach Latent Mosaic Viroid: Nucleotide Sequence of an Italian Isolate, Sensitive Detection Using Rt-PCR and Geographic Distribution

Shamloul, A. M.; Minafra, A.; Hadidi, A.; Waterworth, H. E.; Giunchedi, L.; Allam, E. K.

doi:10.17660/actahortic.1995.386.75

Cited by 50 publications

(40 citation statements)

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“…The (Kofalvi et al 1997) for the HSVd isolate analyzed in this work, the HSVd isolates apr10 and c1 were selected as reference sequences occurrence of HSVd is a new report for Serbia, whereas detection of PLMVd confirmed previous reports from former Yugoslavia in peach but not in plum (Shamloul et al 1995;Hadidi et al 1997). PLMVd was found in peach cultivars originating from the main world breeding centres for this crop.…”

Section: Discussionsupporting

confidence: 63%

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Incidence and genetic diversity of Peach latent mosaic viroid and Hop stunt viroid in stone fruits in Serbia

et al. 2007

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Tissue-imprint hybridization (TIH) assay was validated for large-scale detection of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). All 72 collected leaves (100%) from 2 PLMVd-and 2 HSVd-infected trees were positive in TIH, regardless of the geographic orientation of the scaffold, level of the canopy and position of the leaf in the shoot. In a large-scale survey in Serbia, we tested by TIH 871 trees of stone fruits, representing 602 cultivars from fruit collections in Belgrade, Čačak and Novi Sad. PLMVd was detected in 185 (50%) peach trees or 95 (54%) cultivars and HSVd in 2 apricot trees and cultivars (2%). The occurrence of HSVd is a new report for Serbia. No viroid infection was found in European plums, sweet cherries, sour cherries and wild Prunus spp. PLMVd-infected peach cultivars originated from the world's main breeding centres of this crop.Western European and Asian cultivars were the most infected (58%) followed by those originating from North America (50%). Nine PLMVd and two HSVd isolates were sequenced and analyzed. All showed PMLVd sequences clustered together in the previously reported phylogenetic group III. Both HSVd isolates were found to be derived from recombinant events, but that of the cv. Saturn represented a putative new phylogenetic group of HSVd.

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Section: Discussionsupporting

confidence: 63%

“…PLMVd has been reported to occur in the former Yugoslavia in peach and plum (Shamloul et al 1995;Hadidi et al 1997). However, this survey was very limited.…”

Section: Introductionmentioning

confidence: 99%

Incidence and genetic diversity of Peach latent mosaic viroid and Hop stunt viroid in stone fruits in Serbia

et al. 2007

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“…To obtain a full-length amplified PCR product, PLMVd-c (5' -AAC TGC AGT GCT CCG T-3') and PLMVd-h (5' -CCC GAT AGA AAG GCT AAG CAC CTC G-3') primers were used (Shamloul et al, 1995). The RT reaction was performed at 50°C for 30 min, followed by initial PCR activation step at 95°C for 15 min.…”

Section: Rt-pcr Amplificationmentioning

confidence: 99%

Low incidence of peach latent mosaic viroid in peach mother blocks in Serbia

Jevremović

Paunović

2014

PESTIC PHYTOMED

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SUMMARYPeach latent mosaic viroid (PLMVd) is the causal agent of peach latent mosaic disease that is common on peaches and nectarines worldwide. Most of the isolates do not cause any symptoms on the foliage and the disease may be latent for years. A survey to investigate the presence of PLMVd in selected peach mother blocks in 9 Serbian districts was carried out in 2011 through 2013. A total of 315 trees/samples originating from 43 mother blocks, representing 35 peach and nectarine varieties and 2 rootstocks, were tested by Reverse Transcription -Polymerase Chain Reaction (RT-PCR). PLMVd was detected in 13 samples (4.13%) belonging to 7 varieties and one vineyard peach rootstock. Infected samples were found in 7 mother blocks from 3 districts. Our results indicated a low incidence of PLMVd in the analyzed peach mother blocks.

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“…PLMVd is found across the world and occurs in most commercial peach varieties (Desvignes 1986;Flores and Llacer 1988;Albanese et al 1992;Shamloul et al 1995;Ozaki et al 1999;Kyriakopoulou et al 2001;Mandic et al 2008), where it can cause heavy losses (Faggioli et al 2003). The main symptoms on peach trees include: irregularly shaped, discoloured and deformed fruits with cracked sutures and enlarged pits, bud necrosis, delay in bud-burst, flowering and fruit ripening, decline and reduced longevity of the plants.…”

Section: Introductionmentioning

confidence: 99%

“…Subsequently, the polyacrylamide gel electrophoresis analysis of RNA extracts allowed for the identification and the molecular characterisation of the viroid (Flores and Llacer 1988;Flores et al 1990). After complete sequencing of the PLMVd genome (Hernández and Flores 1992), different molecular methods have been applied for its detection: dot-blot hybridisation with radioactive and non-radioactive probes (Ambros et al 1995;Loreti et al 1995), tissue imprint hybridisation (Loreti et al 1999;Mandic et al 2008); single and multiplex reverse transcription polymerase chain reaction (RT-PCR) (Loreti et al 1999;Ragozzino et al 2004;Shamloul et al 1995;Shamloul and Hadidi 1999), RT-PCR capture hybridisation (Shamloul et al 2002) and loop mediated isothermal amplification (RT-LAMP) (Boubourakas et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Development of quantitative real-time RT-PCR for the detection and quantification of Peach latent mosaic viroid

Luigi

Faggioli

2011

Eur J Plant Pathol

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Peach latent mosaic viroid (PLMVd) is a damaging pathogen for peach, its detection being of critical importance to both sanitary and certification programs worldwide. Here we report a quantitative realtime reverse transcription polymerase chain reaction assay (qRT-PCR) based on TaqMan® chemistry to improve the diagnosis of this pathogen. Critical to this approach is the design of a specific set of primers and of a probe, and the use of a suitable extraction method proving a reliable, sensitive and specific PLMVd diagnosis. More specifically, the sensitivity was evaluated using 12 ten-fold dilution series of an in vitro transcript of the entire PLMVd genome as a standard for quantification in infected samples. The assay detected up to 1 fg of target RNA. The protocol was also evaluated for its specificity using healthy peach, apricot, plum and pear controls and non-target viroids (Apple scar skin viroid, Hop stunt viroid, Pear blister canker viroid, Apple dimple fruit viroid and Potato spinder tuber viroid). None of the non-target or healthy analysed samples reacted in qRT-PCR. The efficiency and accuracy of the method was evaluated using different PLMVd peach isolates as templates, including three 'calico' variants: a strong signal was obtained from all of them. Finally, five RNA extraction methods were compared and evaluated to choose the best for detection and quantification of PLMVd by the qRT-PCR protocol.

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Peach Latent Mosaic Viroid: Nucleotide Sequence of an Italian Isolate, Sensitive Detection Using Rt-PCR and Geographic Distribution

Cited by 50 publications

References 0 publications

Incidence and genetic diversity of Peach latent mosaic viroid and Hop stunt viroid in stone fruits in Serbia

Incidence and genetic diversity of Peach latent mosaic viroid and Hop stunt viroid in stone fruits in Serbia

Low incidence of peach latent mosaic viroid in peach mother blocks in Serbia

Development of quantitative real-time RT-PCR for the detection and quantification of Peach latent mosaic viroid

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