Peach Latent Mosaic Viroid Isolated from Stone Fruits in Japan.

Osaki, Hideki; Yamaguchi, Masami; Satō, Yutaka; Tomita, Yasunori; Kawai, Yasumitsu; Miyamoto, Yoshiaki; Ohtsu, Yoshihiro

doi:10.3186/jjphytopath.65.3

Cited by 15 publications

(15 citation statements)

References 9 publications

(1 reference statement)

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“…Apple trees can be infected by Apple scar skin viroid (ASSVd), which has a major impact on fruit production. The agent occurs in various countries in Europe, America and Asia (Flores et al 1990;Yang et al 1992a;Hadidi et al 1997;Osaki et al 1999;Kyriakopoulou et al 2001) but has not been reported in Turkey yet.…”

Section: Development Of a Rapid Enzymatic Cdna Amplification Test Formentioning

confidence: 93%

Development of a rapid enzymatic cDNA amplification test for the detection of Apple Scar Skin Viroid (ASSVd) in apple trees from eastern Anatolia, Turkey

Sipahioğlu

Usta

Ocak

2009

Archives Of Phytopathology And Plant Protection

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A validated RT-PCR method was used to investigate the presence of Apple scar skin viroid (ASSVd) in the commercial apple orchards of eastern Anatolia. Among three modified and simplified silica-capture based extraction methods, one was used for mass extraction to ascertain the presence of ASSVd. The test was initially performed from an ASSVd-infected source and then applied to total RNA preparations from fresh leaf tissues of apple trees collected from eastern Anatolia. ASSVd was found to occur in apple trees. Among 263 apple samples, 121 were positive for ASSVd. The infected trees showed no apparent disease symptoms on the leaves other than scarring on fruit skin. Overall incidence of ASSVd was 46% in eastern Anatolia. The presence of ASSVd reported for the first time in Turkey. Among three total RNA preparation methods, Method II was determined to be the best procedure for large scale routine analysis. The improved test can be used in a certification or clean stock program to contribute to the prevention of the spread of ASSVd in the eastern Anatolia.

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Section: Development Of a Rapid Enzymatic Cdna Amplification Test Formentioning

confidence: 93%

Development of a rapid enzymatic cDNA amplification test for the detection of Apple Scar Skin Viroid (ASSVd) in apple trees from eastern Anatolia, Turkey

Sipahioğlu

Usta

Ocak

2009

Archives Of Phytopathology And Plant Protection

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“…PLMVd is found across the world and occurs in most commercial peach varieties (Desvignes 1986;Flores and Llacer 1988;Albanese et al 1992;Shamloul et al 1995;Ozaki et al 1999;Kyriakopoulou et al 2001;Mandic et al 2008), where it can cause heavy losses (Faggioli et al 2003). The main symptoms on peach trees include: irregularly shaped, discoloured and deformed fruits with cracked sutures and enlarged pits, bud necrosis, delay in bud-burst, flowering and fruit ripening, decline and reduced longevity of the plants.…”

Section: Introductionmentioning

confidence: 98%

Development of quantitative real-time RT-PCR for the detection and quantification of Peach latent mosaic viroid

Luigi

Faggioli

2011

Eur J Plant Pathol

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Peach latent mosaic viroid (PLMVd) is a damaging pathogen for peach, its detection being of critical importance to both sanitary and certification programs worldwide. Here we report a quantitative realtime reverse transcription polymerase chain reaction assay (qRT-PCR) based on TaqMan® chemistry to improve the diagnosis of this pathogen. Critical to this approach is the design of a specific set of primers and of a probe, and the use of a suitable extraction method proving a reliable, sensitive and specific PLMVd diagnosis. More specifically, the sensitivity was evaluated using 12 ten-fold dilution series of an in vitro transcript of the entire PLMVd genome as a standard for quantification in infected samples. The assay detected up to 1 fg of target RNA. The protocol was also evaluated for its specificity using healthy peach, apricot, plum and pear controls and non-target viroids (Apple scar skin viroid, Hop stunt viroid, Pear blister canker viroid, Apple dimple fruit viroid and Potato spinder tuber viroid). None of the non-target or healthy analysed samples reacted in qRT-PCR. The efficiency and accuracy of the method was evaluated using different PLMVd peach isolates as templates, including three 'calico' variants: a strong signal was obtained from all of them. Finally, five RNA extraction methods were compared and evaluated to choose the best for detection and quantification of PLMVd by the qRT-PCR protocol.

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“…In peach ( Prunus persicae ), PLMVd can induce a broad variety of symptoms: mosaic, large white patches covering most of the leaf blade, irregularly shaped colourless fruits with cracked sutures and enlarged pits, bud necrosis and delay in foliation, flowering and maturity and a general wilting (Desvignes, 1986). The incidence of PLMVd reached up to 60% in North American germplasm (Shamloul et al., 1995; Hadidi et al., 1997), 40% in Syrian germplasm (Ismaeil et al., 2001), 50% in Italian germplasm (Loreti et al., 1998), 64% in Chinese germplasm (Zhang et al., 2000), 68% in Australian germplasm (Di Serio et al., 1999), 80–85% in Spanish germplasm (Flores et al., 1992) and 90% in Japanese germplasm (Osaki et al., 1999). In orchards, the infection rate of commercial varieties of peach and nectarine in five USA states reached 50% (Skrzeczkowski et al., 1996).…”

Section: Introductionmentioning

confidence: 99%

Distribution of Peach latent mosaic viroid in Commercial Orchards of Peach in the North of Tunisia

Hassen¹,

Massart²,

Roussel³

et al. 2007

Journal of Phytopathology

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The presence of Peach latent mosaic viroid (PLMVd) was monitored during 2 years in peach orchards located in the North of Tunisia. PLMVd population was surveyed using a specific RT-PCR test adapted to crude sap extract. During the first year (2004), 228 samples were randomly selected in 22 peach orchards (eight cultivars) and tested for the presence of PLMVd. Results showed that PLMVd is highly and equally present in four regions of the North of Tunisia. Analyses of some key factors in relation to PLMVd incidence revealed that the tree age did not influence the infection rate. The eight studied cultivars were clustered in three groups according to their PLMVd incidence. Furthermore, the early and season cultivars were statistically more infected than the late cultivars. Prospections in May and October 2005 were performed in four selected orchards. Each two orchards contained Early May Crest (early cultivar) and Carnival (late cultivar) cultivars, respectively. The difference in PLMVd incidence observed in 2004 was confirmed. Furthermore, no correlation between the tree physiological state and the viroid detection was observed. Further investigations on the origin of the differences in PLMVd incidence between Early May Crest and Carnival cultivars would be of interest.

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Peach Latent Mosaic Viroid Isolated from Stone Fruits in Japan.

Cited by 15 publications

References 9 publications

Development of a rapid enzymatic cDNA amplification test for the detection of Apple Scar Skin Viroid (ASSVd) in apple trees from eastern Anatolia, Turkey

Development of a rapid enzymatic cDNA amplification test for the detection of Apple Scar Skin Viroid (ASSVd) in apple trees from eastern Anatolia, Turkey

Development of quantitative real-time RT-PCR for the detection and quantification of Peach latent mosaic viroid

Distribution of Peach latent mosaic viroid in Commercial Orchards of Peach in the North of Tunisia

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