Peace, a MYB-like transcription factor, regulates petal pigmentation in flowering peach ‘Genpei’ bearing variegated and fully pigmented flowers

Uematsu, Chiyomi; Katayama, Hironori; Makino, Izumi; Inagaki, Azusa; Arakawa, Osamu; Martin, Cathie

doi:10.1093/jxb/ert456

Cited by 53 publications

(49 citation statements)

References 61 publications

(83 reference statements)

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“…These results suggest that PpMYB10 plays a different role in the anthocyanin regulation of the two blood-flesh peaches. Moreover, some members of MYB10 are thought to be involved in regulating anthocyanin biosynthesis (Rahim et al, 2014;Uematsu et al, 2014). Further studies should focus on their responsiveness to both developmental and environmental stimuli in blood-flesh peaches.…”

Section: Discussionmentioning

confidence: 99%

Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development

Jiao

Shen

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:The blood-flesh peach has become popular in China due to its attractive anthocyanin-induced pigmentation and antioxidant properties. In this study, we investigated the molecular mechanisms underlying anthocyanin accumulation by examining the expression of nine genes of the anthocyanin biosynthesis pathway found in the peach mesocarp. Expression was measured at six developmental stages in fruit of two blood-flesh and one white-flesh peach cultivars, using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results show that the expression of the chalcone synthase (CHS) gene was closely related to anthocyanin accumulation in both of the blood-flesh peaches. In the white-flesh peach, we found that the transcription level of phenylalanine ammonia-lyase (PAL) during fruit development was much lower than that in the blood-flesh peach, even though all other genes of the anthocyanin biosynthesis pathway were highly expressed, suggesting that the PAL gene may be limiting in anthocyanin production in the white-flesh peach. Moreover, the transcription levels of the CHS and UDP-glucose-flavonoid 3-O-glucosyltransferase (UFGT) genes were markedly up-regulated at three days after bag removal (DABR) in the blood-flesh peach, suggesting that CHS and UFGT are the key genes in the process of anthocyanin biosynthesis for both of the blood-flesh peaches. The present study will be of great help in improving understanding of the molecular mechanisms involved in anthocyanin accumulation in blood-flesh peaches.

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Section: Discussionmentioning

confidence: 99%

Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development

Jiao

Shen

et al. 2014

J. Zhejiang Univ. Sci. B

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“…Differential expression of Peace ( peach anthocyanin colour enhancement , a R2R3 MYB-like gene) determines the pattern of flower coloration in variegated petals within individual trees of flowering P. persica “Genpei” [25]. In addition, jasmonate has been reported to regulate WD-repeat/bHLH/MYB complex-mediated anthocyanin accumulation in Arabidopsis thaliana [58].…”

Section: Discussionmentioning

confidence: 99%

Substantial Epigenetic Variation Causing Flower Color Chimerism in the Ornamental Tree Prunus mume Revealed by Single Base Resolution Methylome Detection and Transcriptome Sequencing

Zhang

Cheng

et al. 2018

IJMS

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Epigenetic changes caused by methylcytosine modification participate in gene regulation and transposable element (TE) repression, resulting in phenotypic variation. Although the effects of DNA methylation and TE repression on flower, fruit, seed coat, and leaf pigmentation have been investigated, little is known about the relationship between methylation and flower color chimerism. In this study, we used a comparative methylomic–transcriptomic approach to explore the molecular mechanism responsible for chimeric flowers in Prunus mume “Danban Tiaozhi”. High-performance liquid chromatography-electrospray ionization mass spectrometry revealed that the variation in white (WT) and red (RT) petal tissues in this species is directly due to the accumulation of anthocyanins, i.e., cyanidin 3,5-O-diglucoside, cyanidin 3-O-glucoside, and peonidin 3-O-glucoside. We next mapped the first-ever generated methylomes of P. mume, and found that 11.29–14.83% of the genomic cytosine sites were methylated. We also determined that gene expression was negatively correlated with methylcytosine level in general, and uncovered significant epigenetic variation between WT and RT. Furthermore, we detected differentially methylated regions (DMRs) and DMR-related genes between WT and RT, and concluded that many of these genes, including differentially expressed genes (DEGs) and transcription factor genes, are critical participants in the anthocyanin regulatory pathway. Importantly, some of the associated DEGs harbored TE insertions that were also modified by methylcytosine. The above evidence suggest that flower color chimerism in P. mume is induced by the DNA methylation of critical genes and TEs.

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“…Moreover, UFGT is a key gene in anthocyanin biosynthesis, and its expression is induced by MYB TFs (Kobayashi et al, 2002). To date, seven R2R3 MYB TFs have been identified in the peach genome (Rahim et al, 2014;Uematsu et al, 2014). It is worth studying whether the expression of PpUGT78 genes is induced by specific MYB TFs, leading to the spatial and temporal patterns of anthocyanin synthesis in peach.…”

Section: Differential Expression Pattern Of Genes Involved In Anthocymentioning

confidence: 99%

Unraveling the Mechanism Underlying the Glycosylation and Methylation of Anthocyanins in Peach

et al. 2014

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Modification of anthocyanin plays an important role in increasing its stability in plants. Here, six anthocyanins were identified in peach (Prunus persica), and their structural diversity is attributed to glycosylation and methylation. Interestingly, peach is quite similar to the wild species Prunus ferganensis but differs from both Prunus davidiana and Prunus kansueasis in terms of anthocyanin composition in flowers. This indicates that peach is probably domesticated from P. ferganensis. Subsequently, genes responsible for both methylation and glycosylation of anthocyanins were identified, and their spatiotemporal expression results in different patterns of anthocyanin accumulation in flowers, leaves, and fruits. Two tandem-duplicated genes encoding flavonoid 3-O-glycosyltransferase (F3GT) in peach, PpUGT78A1 and PpUGT78A2, showed different activity toward anthocyanin, providing an example of divergent evolution of F3GT genes in plants. Two genes encoding anthocyanin O-methyltransferase (AOMT), PpAOMT1 and PpAOMT2, are expressed in leaves and flowers, but only PpAOMT2 is responsible for the O-methylation of anthocyanins at the 39 position in peach. In addition, our study reveals a novel branch of UGT78 genes in plants that lack the highly conserved intron 2 of the UGT gene family, with a great variation of the amino acid residue at position 22 of the plant secondary product glycosyltransferase box. Our results not only provide insights into the mechanisms underlying anthocyanin glycosylation and methylation in peach but will also aid in future attempts to manipulate flavonoid biosynthesis in peach as well as in other plants.

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Peace, a MYB-like transcription factor, regulates petal pigmentation in flowering peach ‘Genpei’ bearing variegated and fully pigmented flowers

Abstract: Summary Peace expression determined flower colouration whether pigmented or variegated. In spite of the close relationship between Peace and AtMYB123, a proanthocyanidin regulator, Peace regulates anthocyanin biosynthesis in flowering peach.

Cited by 53 publications

References 61 publications

Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development

Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development

Substantial Epigenetic Variation Causing Flower Color Chimerism in the Ornamental Tree Prunus mume Revealed by Single Base Resolution Methylome Detection and Transcriptome Sequencing

Unraveling the Mechanism Underlying the Glycosylation and Methylation of Anthocyanins in Peach

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