PEA3 activates VEGF transcription in T47D and SKBR3 breast cancer cells

Hua, Dong; Chen, Bobin; Bai, Mei; Yu, Hao; Wu, Xiaohong; Jin, Wei

doi:10.1093/abbs/gmn007

Cited by 15 publications

(15 citation statements)

References 21 publications

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“…Previous reports indicated transfection with PEA3 resulted in enhanced motility and invasion in lung cancer cells and human SKBR3 breast cancer cells [15,16]. PEA3 was found to activate VEGF transcription in T47D and SKBR3 breast cancer cells [17]. PEA3 has a close correlation to cancer characteristic, but there are no reports about the interaction between PEA3 and CXCR4 transcription in breast cancer.…”

Section: Discussionmentioning

confidence: 93%

PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells

Gu¹,

Chen²,

Qi³

et al. 2011

ABBS

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CXC chemokine receptor 4 (CXCR4) is a cell surface receptor that has been shown to mediate the metastasis of many solid tumors including lung, breast, kidney, and prostate tumors. In this study, we found that overexpression of ets variant gene 4 (PEA3) could elevate CXCR4 mRNA level and CXCR4 promoter activity in human MDA-MB-231 and MCF-7 breast cancer cells. PEA3 promoted CXCR4 expression and breast cancer metastasis. Chromatin immunoprecipitation assay demonstrated that PEA3 could bind to the CXCR4 promoter in the cells transfected with PEA3 expression vector. PEA3 siRNA attenuated CXCR4 promoter activity and the binding of PEA3 to the CXCR4 promoter in MDA-MB-231 and MCF-7 cells. These results indicated that PEA3 could activate CXCR4 promoter transcription and promote breast cancer metastasis.

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Section: Discussionmentioning

confidence: 93%

PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells

Gu¹,

Chen²,

Qi³

et al. 2011

ABBS

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“…35,36) Deregulation of PEA3 is associated with a variety of cancers including colon, breast, ovarian, prostate, and esophageal cancer. [37][38][39] ESE-1 (also called ELF3, ESX, jen, and ERT) is expressed in various cancer-derived cell lines, as well as in epithelial cellrich tissues, and exerts multiple roles in pathophysiological processes. [40][41][42][43] Null mutation of Elf3, the mouse homolog of human ESE-1, increases the risk of embryonic death in utero, suggesting that ESE-1 plays an important role in embryonic development.…”

Section: Resultsmentioning

confidence: 99%

Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation

Park

et al. 2014

Biological & Pharmaceutical Bulletin

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E26 transformation-specific (ETS) transcription factors play important roles in normal and tumorigenic processes during development, differentiation, homeostasis, proliferation, and apoptosis. To identify critical ETS factor(s) in germ cell-derived cancer cells, we examined the expression patterns of the 27 ETS transcription factors in naive and differentiated NCCIT human embryonic carcinoma cells, which exhibit both pluripotent and tumorigenic characteristics. Overall, expression of ETS factors was relatively low in NCCIT cells. Among the 27 ETS factors, polyomavirus enhancer activator 3 (PEA3) and epithelium-specific ETS transcription factor-1 (ESE-1) exhibited the most significant changes in their expression levels. Western blot analysis confirmed these patterns, revealing reduced levels of PEA3 protein and elevated levels of ESE-1 protein in differentiated cells. PEA3 increased the proportion of cells in S-phase and promoted cell growth, whereas ESE-1 reduced proliferation potential. These data suggest that PEA3 and ESE-1 may play important roles in pluripotent and tumorigenic embryonic carcinoma cells. These findings contribute to our understanding of the functions of oncogenic ETS factors in germ cell-derived stem cells during processes related to tumorigenesis and pluripotency.

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“…PEA3 has been shown to control the expression of several matrix metalloproteases, including MMP-1 [23,36] and MMP-7 [24], and other genes such as osteopontin [37] and VEGF [38]. We therefore examined whether PEA3 presence correlated with expression of any of these potential targets in the cell line models.…”

Section: Resultsmentioning

confidence: 99%

The ERK MAP kinase-PEA3/ETV4-MMP-1 axis is operative in oesophageal adenocarcinoma

et al. 2010

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BackgroundMany members of the ETS-domain transcription factor family are important drivers of tumourigenesis. In this context, their activation by Ras-ERK pathway signaling is particularly relevant to the tumourigenic properties of many ETS-domain transcription factors. The PEA3 subfamily of ETS-domain transcription factors have been implicated in tumour metastasis in several different cancers.ResultsHere, we have studied the expression of the PEA3 subfamily members PEA3/ETV4 and ER81/ETV1 in oesophageal adenocarcinomas and determined their role in oesophageal adenocarcinoma cell function. PEA3 plays an important role in controlling both the proliferation and invasive properties of OE33 oesophageal adenocarcinoma cells. A key target gene is MMP-1. The ERK MAP kinase pathway activates PEA3 subfamily members and also plays a role in these PEA3 controlled events, establishing the ERK-PEA3-MMP-1 axis as important in OE33 cells. PEA3 subfamily members are upregulated in human adenocarcinomas and expression correlates with MMP-1 expression and late stage metastatic disease. Enhanced ERK signaling is also more prevalent in late stage oesophageal adenocarcinomas.ConclusionsThis study shows that the ERK-PEA3-MMP-1 axis is upregulated in oesophageal adenocarcinoma cells and is a potentially important driver of the metastatic progression of oesophageal adenocarcinomas.

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PEA3 activates VEGF transcription in T47D and SKBR3 breast cancer cells

Cited by 15 publications

References 21 publications

PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells

PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells

Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation

The ERK MAP kinase-PEA3/ETV4-MMP-1 axis is operative in oesophageal adenocarcinoma

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