Pea‐Stem Sections as Test Object for Indole‐3‐Acetaldehyde

Andersen, A. Skytt

doi:10.1111/j.1399-3054.1964.tb08214.x

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Segments of pea stems are known to respond with increased growth to IAA but not to IAN (2,21,24). If the Avena curvature and growlh of the green hypocotyl tis.sue is the result of c(mversiou of glucobrassicin to IAN by myroshiase (0, 14) and of IAN to IAA by nitrilase (17.…”

Section: Resultsmentioning

confidence: 99%

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Auxin Activity of Glucobrassicin

Andersen

Muir

1966

Physiologia Plantarum

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The indole‐glucosinolate, glucobrassicin, which is found in large amounts in members of the genus Brassica, was subjected to tests in the standard Avena curvature bioassay. Both the tetramethylammonium salt of glucobrassicin and the non‐crystallizted material induced curvature at a concentration of 5 × l0−4M. The curvature from the salt was, however, only half of that induced by the acid form. Bioassays of chromatograms of fresh methanolic extracts of savoy cabbage indicated the presence of indole‐3‐acetic acid as well as indole‐3‐acetonitrile in small amounts along with large amounts of glucobrassicin. Segments of green hypocotyl tissue of savoy cabbage respond with limited growth to high concentrations of glucobrassicin. Segments of etiolated hypocotyls or green epicotyls give no growth response to glucobrassicin. Pea stem segments which do not respond to indoleacetonitrile responded with similar growth to glucobrassicin and indoleacetic acid. Nitrilase activity was found in savoy hypocotyl and epicotyl tissue by chromatography of incubation media. The growth regulation in savoy cabbage in conjunction with data on the distribution of glucobrassicin in the plant, leads to the conclusion that glucobrassicin is a special, inactive storage and transport form of the active auxin in cabbage.

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Section: Resultsmentioning

confidence: 99%

“…The growth response of the epicotyl tissue of the Alaska pea was determined for segments, 5 mm in length, from the third internode of plants grown either in the dark or in the greenhouse for seven days. The assay was carried out as descrihed earlier (21).…”

Section: Methodsmentioning

confidence: 99%

Auxin Activity of Glucobrassicin

Andersen

Muir

1966

Physiologia Plantarum

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Growth Responses of Etiolated Crucifer Hypocotyl Sections to Intlole‐3‐Acetic Acid, Indole‐3–Acetonitrile and Indole‐3‐Acetaldehyde

Andersen

1964

Physiologia Plantarum

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Interactions between Plants and Epiphytic Bacteria Regarding Their Auxin Metabolism

Wichner

Libbert

1968

Physiologia Plantarum

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Proofs of different kind are presented of the existence of highly active bacteria producing IAA from tryptophan on plant surfaces and in plant homogenates. Both homogenates and washing solutions of nonsterile pea plant parts are active in producing IAA from tryptophan. Activity is much enhanced by the addition of glucose or by preincubating the preparations; it is abolished by sterile filtration, by some bactericidic and bacteriostatic substances, by chloramphenicol, streptomycin, and albucid (penicillin being only partly effective). Preparations of sterile plants do not produce IAA from tryptophan within the detection limit of the Salkowski test. The bacteria are even present on seed surfaces, in the air, and in aceton or ammonium sulfate precipitations of homogenates. Main products of the bacterial tryptophan conversion, as demonstrated by paper chromatography, are indolepyruvic acid, indoleacetic acid, indolecarboxaldehyde, and indolecarboxylic acid. In presence of glucose indolepyruvic acid is by far dominating. Many hitherto known results about tryptophan conversion to IAA by higher plants are likely to be falsified by epiphytic bacteria.

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Pea‐Stem Sections as Test Object for Indole‐3‐Acetaldehyde

Cited by 3 publications

References 25 publications

Auxin Activity of Glucobrassicin

Auxin Activity of Glucobrassicin

Growth Responses of Etiolated Crucifer Hypocotyl Sections to Intlole‐3‐Acetic Acid, Indole‐3–Acetonitrile and Indole‐3‐Acetaldehyde

Interactions between Plants and Epiphytic Bacteria Regarding Their Auxin Metabolism

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