Pea protease inhibitor inhibits protease activity and development of Bactrocera cucurbitae

Kaur, Amrit Pal; Sohal, Satwinder Kaur

doi:10.1016/j.aspen.2016.10.012

Cited by 13 publications

(7 citation statements)

References 45 publications

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“…The insecticidal potential of a PI from Pisum sativum seeds showed important effects on the development of Bactrocera cucurbitae (Diptera) larvae, including prolongation of the larval and pupal stages, a decrease in pupal weight and a reduction in adult emergence. This inhibitor reduced the activity of trypsin, chymotrypsin, leucine aminopeptidase and elastase . Developmental impairment in dipteran insects was also reported in a study that investigated the effects of the trypsin inhibitor from Moringa oleifera flowers (MoFTI) on Aedes aegypti , the first demonstration of the effect of a PI against this mosquito.…”

Section: Targets and Insecticidal Effects Of Protease Inhibitorsmentioning

confidence: 62%

Insect midgut structures and molecules as targets of plant‐derived protease inhibitors and lectins

Napoleão

Albuquerque

Santos

et al. 2018

Pest Management Science

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The midgut of insects is involved in digestion, osmoregulation and immunity. Although several defensive strategies are present in this organ, its organization and function may be disturbed by some insecticidal agents, including bioactive proteins like lectins and protease inhibitors (PIs) from plants. PIs interfere with digestion, leading to poor nutrient absorption and decreasing amino acid bioavailability. Intake of PIs can delay development, cause deformities and reduce fertility. Ingestion of PIs may lead to changes in the set of proteases secreted in the insect gut, but this response is often insufficient and results in aggravation of the malnutrition status. Lectins are proteins that are able to interact with glycoconjugates, including those linked to cell surfaces. Their effects on the midgut include disruption of the peritrophic matrix, brush border and secretory cell layer; induction of apoptosis and oxidative stress; interference with nutrient absorption and transport proteins; and damaging effects on symbionts. In addition, lectins can cross the intestinal barrier and reach the hemolymph. The establishment of resistant insect populations due to selective pressure resulting from massive use of a bioactive protein is an actual possibility, but this can be minimized by the multiple mode-of-action of these proteins, mainly the lectins.

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Section: Targets and Insecticidal Effects Of Protease Inhibitorsmentioning

confidence: 62%

Insect midgut structures and molecules as targets of plant‐derived protease inhibitors and lectins

Napoleão

Albuquerque

Santos

et al. 2018

Pest Management Science

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“…The activity of trypsin and chymotrypsin decreased at LC 50 concentration of the SMTI. A previous study reported inhibition in trypsin and chymotrypsin activity in B. cucurbitae larvae by the action of peptidase inhibitor extracted from peas 30 . Silva et al .…”

Section: Discussionmentioning

confidence: 96%

“…The decrease in growth rate of B. cucurbitae larvae indicated by decline in percentage pupation and percentage emergence suggests a deterrent effect of the SMTI on the development of the 2 nd instar larvae (64–72 h) of melon fruit fly. Kaur and Sohal 30 , perceived that different concentrations of the pea peptidase inhibitor, when added in artificial diet of second instar larvae (64–72 h) of B. cucurbitae , decreased the larval growth and total development period. Results analogous to present findings were also observed by Kaur et al .…”

Section: Discussionmentioning

confidence: 99%

Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

Samiksha

Singh

Kesavan

et al. 2019

Sci Rep

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Peptidase inhibitors (PIs) are defense proteins of plants which are active against gut peptidases of different insects. Sapindus mukorossi was identified as a source of bioactive PIs which could confer resistance against Bactrocera cucurbitae, a most devastating pest of several economically important crops. In the present study, a trypsin inhibitor was purified from mature dry seeds of S. mukorossi and characterized for its biochemical properties as well as its potential for bio control of B. cucurbitae. The purified fractions from RP- HPLC through SDS-PAGE gave an apparent molecular weight of ~29 kDa. S. mukorossi trypsin inhibitor (SMTI) was found to be a non-competitive inhibitor which was active over a broad range of temperature (10–100 °C) and pH (6–11). SMTI when incorporated in artificial diet inhibited the growth and development of B. cucurbitae larvae. Gene expression analysis of trypsin and chymotrypsin genes via qRT-PCR indicated that their mRNA expression was down-regulated while that of other genes namely, Catalase, Elastase, Superoxide Dismutase, Glutathione –S-transferase and Alkaline Phosphatase was up regulated. SMTI also showed deleterious effects against different bacterial strains. The results of this study indicated that S. mukorossi trypsin inhibitor has potential to be used as a bio control agent that can reduce the harm caused by melon fruit fly and other devastating pests.

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“…The winged bean PI had an inhibitory effect on the percentage pupation and percentage emergence of B. cucurbitae larvae. A reduction in pupation and adult emergence of melon fruit fly was also observed when larval instars were fed on diet containing SBTI, SBBI, and pea PI (Kaur et al, 2009(Kaur et al, , 2010Kaur & Sohal, 2016). A significant effect on the growth inhibition and mortality of the pea aphid, Acyrthosiphon pisum (Harris) (Rahbe et al, 2003), was noticed when the nymphs were fed on pea trypsin-chymotrypsin inhibitors (P S TI-2) of the Bowman-Birk family.…”

Section: Effect Of Winged Bean Pi On Larval Growthmentioning

confidence: 99%

Purified winged bean protease inhibitor affects the growth ofBactrocera cucurbitae

Kaur

Sohal

2018

Bull. Entomol. Res.

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The melon fruit fly, Bactrocera cucurbitae (Coquillett), is a serious agricultural pest which has defied the various control measures employed against it. Protease inhibitors present in plants which have the potential to inhibit the growth and development of associated insect pests can be a possible alternative which can be manipulated for developing resistance in plants to the pest. In the present study, winged bean (Psophocarpus tetragonolobus) protease inhibitor isolated through affinity chromatography was explored for its potential to disrupt the development of melon fruit fly, B. cucurbitae. Different concentrations (12.5, 25, 50, 100, 200, and 400 µg ml−1) of the winged bean protease inhibitor (WBPI) were incorporated into the artificial diet of the second instar (64–72 h old) larvae of B. cucurbitae. The WBPI significantly delayed the larval, pupal, and total development period. The percentage pupation and adult emergence of the treated larvae was reduced as compared with control. The activities of major digestive enzymes (trypsin, chymotrypsin, leucine aminopeptidase, and elastase) decreased significantly in the larvae treated with different concentrations (50, 100, 200, and 400 µg ml−1) of WBPI. The findings reveal that the inhibitor holds considerable promise for the management of the melon fruit fly.

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Pea protease inhibitor inhibits protease activity and development of Bactrocera cucurbitae

Cited by 13 publications

References 45 publications

Insect midgut structures and molecules as targets of plant‐derived protease inhibitors and lectins

Insect midgut structures and molecules as targets of plant‐derived protease inhibitors and lectins

Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

Purified winged bean protease inhibitor affects the growth ofBactrocera cucurbitae

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