2016
DOI: 10.1038/srep21624
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PE11, a PE/PPE family protein of Mycobacterium tuberculosis is involved in cell wall remodeling and virulence

Abstract: The role of the unique proline-glutamic acid (PE)/proline-proline-glutamic acid (PPE) family of proteins in the pathophysiology and virulence of Mycobacterium tuberculosis is not clearly understood. One of the PE family proteins, PE11 (LipX or Rv1169c), specific to pathogenic mycobacteria is found to be over-expressed during infection of macrophages and in active TB patients. In this study, we report that M. smegmatis expressing PE11 (Msmeg-PE11) exhibited altered colony morphology and cell wall lipid composit… Show more

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Cited by 83 publications
(107 citation statements)
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References 58 publications
(93 reference statements)
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“…Reverse transcription qPCR was carried out as described earlier4950. In brief, total RNA was isolated from various tissues of the infected animals using TRI Reagent (Sigma-Aldrich, Bangalore, India) as per manufacturer’s instructions.…”
Section: Methodsmentioning
confidence: 99%
“…Reverse transcription qPCR was carried out as described earlier4950. In brief, total RNA was isolated from various tissues of the infected animals using TRI Reagent (Sigma-Aldrich, Bangalore, India) as per manufacturer’s instructions.…”
Section: Methodsmentioning
confidence: 99%
“…These family members have been confirmed as esterases and modeled onto an α/β-hydrolase protein fold with a classic catalytic triad (Singh et al, 2016;Sultana, Vemula, Banerjee, & Guruprasad, 2013;Vemula, Balakrishnan, Banerjee, & Guruprasad, 2018). The most well-studied of this family-PE11 (Rv1169c or LipX)-plays a role in biofilm growth, creates an immunogenic response, and affects the levels of virulence-related lipids (Rastogi et al, 2017;Singh et al, 2016). The combined effect of PE11 on virulence lipids and biofilm growth suggests a biological role dependent on its esterase activity.…”
Section: Mtb Esterasesmentioning
confidence: 97%
“…These eight α/β-hydrolase PE and PPE domain family members were nicknamed the PE-PPE domain subfamily, as each member has a C-terminal domain, encoding a predicted α/β-hydrolase protein fold and an N-terminal PE or PPE domain (Sultana et al, 2011). These family members have been confirmed as esterases and modeled onto an α/β-hydrolase protein fold with a classic catalytic triad (Singh et al, 2016;Sultana, Vemula, Banerjee, & Guruprasad, 2013;Vemula, Balakrishnan, Banerjee, & Guruprasad, 2018). The most well-studied of this family-PE11 (Rv1169c or LipX)-plays a role in biofilm growth, creates an immunogenic response, and affects the levels of virulence-related lipids (Rastogi et al, 2017;Singh et al, 2016).…”
Section: Mtb Esterasesmentioning
confidence: 99%
“…Moreover, PE_PGRS33 (Gastelum-Avina et al, 2015), PE_PGRS41 (Deng et al, 2017), and PE11 (Singh et al, 2016) are associated with colony morphology alteration. Moreover, PE11 expressing M. smegmatis induced biofilm formation (Singh et al, 2016).…”
Section: Introductionmentioning
confidence: 96%
“…The PE11 (Rastogi et al, 2017), lipY (Santucci et al, 2019), and PE_PGRS33 (Cascioferro et al, 2011), associated with the mycobacterial cell wall and PE domains of PE11 and PE_PGRS33 are responsible for translocation and localization to the cell wall (Cascioferro et al, 2007(Cascioferro et al, , 2011. Moreover, PE_PGRS33 (Gastelum-Avina et al, 2015), PE_PGRS41 (Deng et al, 2017), and PE11 (Singh et al, 2016) are associated with colony morphology alteration. Moreover, PE11 expressing M. smegmatis induced biofilm formation (Singh et al, 2016).…”
Section: Introductionmentioning
confidence: 99%