PDT technology: an alternative to control oral candidiasis in critically ill patients

Alves, Andrea Dias; Gurgel-Juarez, Nalia; Vieira, Andrei Cardoso; Proietti, Aldo Aparecido; Barcessat, Ana Rita Pinheiro

doi:10.1007/s41547-021-00129-8

Cited by 2 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, PDI also has the potential to be used against other microbial targets, such as viruses, parasites or even fungi [ 8 ]. Due to their relevance in many aspects, more and more research is looking into the potential of PDI to combat fungi [ 28 , 35 , 36 , 37 , 38 ]. Therefore, we have shown here that PDI using curcumin and a water-soluble derivate of curcumin (SA-CUR 12a) shows potential as an alternative to conventional treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The use of curcumin in PDI against oral candidiasis has already been tested in immunosuppressed mice showing promising results (4 log reduction with treatment using 80 µM curcumin + 10% DMSO, 37.5 J cm −2 ) [ 41 ]. Additionally, first tests were performed on the clinical application of PDI for the treatment of oral candidiasis [ 37 ] using methylene blue as a photoactive compound. This approach induced a reduction of 1.99 log CFU of Candida spp.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger

Schamberger

Plaetzer

2021

Antibiotics

View full text Add to dashboard Cite

Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the antifungal use of Photodynamic Inactivation (PDI) based on the natural photosensitizer curcumin and a water-soluble positively charged derivative thereof (SA-CUR 12a) against two different model organisms; Candida albicans grown in a liquid culture and photo treated with a 435 nm LED light followed by counting of the colony-forming units and photoinactivation of tissue-like hyphal spheres of Aspergillus niger (diameter ~5 mm) with subsequent monitoring of colony growth. Curcumin (50 µM, no incubation period, i.p.) supplemented with 10% or 0.5% DMSO as well as SA-CUR 12a (50 µM no i.p or 5 min i.p.) triggered a photoantifungal effect of >4 log units towards C. albicans. At 100 µM, SA-CUR 12a (0 min or 5 min i.p.) achieved a reduction of >6 log units. Colonies of A. niger shrunk significantly during PDI treatment. Photoinactivation with 50 µM or 100 µM curcumin (+0.5% DMSO) resulted in complete growth inhibition. PDI using 20, 50 or 100 µM SA-CUR 12a (with or without 10% DMSO) also showed a significant reduction in colony area compared to the control after 48 h, although less pronounced compared to curcumin. In summary, PDI using curcumin or SA-CUR 12a against C. albicans or A. niger is a promising alternative to currently used fungicides, with the advantage of being very unlikely to induce resistance.

show abstract