pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery

Bisht, Savita; Bhakta, Gajadhar; Mitra, Susmita; Maitra, Amarnath

doi:10.1016/j.ijpharm.2004.07.035

Cited by 232 publications

(183 citation statements)

References 31 publications

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“…However, this is not the case if a water/oil/water interfacial reaction route has been used [250]. To extend this subject, inclusion of DNA into CDHA/BSA biocomposites was claimed [250,[693][694][695]. Besides, bionanocomposites of an unspecified calcium orthophosphate with DNA were prepared as well [696].…”

Section: Biocomposites With Collagenmentioning

confidence: 99%

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Dorozhkin¹

2009

J Mater Sci

283

146

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Section: Biocomposites With Collagenmentioning

confidence: 99%

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Dorozhkin¹

2009

J Mater Sci

283

146

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“…The outer calcium phosphate shell provides protection for DNA, and the outer DNA shell provides electrostatic and steric stabilization of the particles. Bisht et al prepared calcium phosphate nanoparticles from microemulsions with a size range of 30-40 nm [20]. In these particles, DNA was encapsulated into the calcium phosphate core and thus protected from the degradation by nucleases.…”

Section: Advanced Bioceramics In Nanomedicine and Tissue Engineeringmentioning

confidence: 99%

Functionalized Calcium Phosphate Nanoparticles for Biomedical Application

Epple

Kovtun

2010

KEM

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Calcium phosphate is a natural biomineral and therefore possesses an excellent biocompatibility due to its chemical similarity to human hard tissue (bone and teeth). Calcium phosphate nanoparticles can be precipitated under controlled conditions and used as carrier in biological systems, e.g. to transfer nucleic acids or drugs. Such nanoparticles can also be suitably functionalized with fluorescing dyes, polymeric agents, pro-drugs or activators. The small monodisperse nanoparticles only mildly influence the intracellular calcium level and therefore are not toxic for cells.

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“…9 More recently, HAp nanoparticles (NPs) have been used as non-viral gene carriers. [10][11][12][13] Thus, DNA-HAp NPs complexes were found to cross cell membranes, integrating into the cell genome. In a very recent study, Brundin et al 14 showed a specific binding affinity of HAp for DNA.…”

Section: Introductionmentioning

confidence: 99%