PDMS microfluidic chips prepared by a novel casting and pre-polymerization method

Schrott, Walter; Svoboda, Miloš; Slouka, Zdeněk; Přibyl, Michal; Šnita, Dalimil

doi:10.1016/j.mee.2009.10.049

Cited by 23 publications

(11 citation statements)

References 16 publications

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“…After alignment and assembly of the first PDMS slab on the second master, the device was heated again at 85 C for 30 minutes to enhance adhesion between two PDMS layers. 43 Injection holes were punched to insert tubing. The resulting PDMS slab was sealed against a glass slide after plasma treatment.…”

Section: Device Fabricationmentioning

confidence: 99%

Controlling the contents of microdroplets by exploiting the permeability of PDMS

et al. 2011

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A microfluidic device capable of exploiting the permeability of small molecules through polydimethylsiloxane (PDMS) has been fabricated in order to control the contents of microdroplets stored in storage wells. We demonstrate that protein precipitation and crystallization can be triggered by delivery of ethanol from a reservoir channel, thus controlling the protein solubility in microdroplets. Likewise quorum sensing in bacteria was triggered by delivery of the auto-inducer N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) through the PDMS membrane of the device.

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Section: Device Fabricationmentioning

confidence: 99%

Controlling the contents of microdroplets by exploiting the permeability of PDMS

et al. 2011

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“…[7][8][9][10] Among them, poly(dimethylsiloxane) (PDMS) has been one of the most widely used materials because it allows rapid fabrication of devices using relatively simple and inexpensive instrumentation. [11][12][13][14] Although the general attributes of PDMS and their molecular bases were recognized many decades ago, 15 it is worth highlighting its chemical inertness, low electrical conductivity, elasticity, 6 and optical transparency. 7,16 PDMS does not swell or dissolve in a number of solvents 17 and is permeable to most gases, including oxygen.…”

Section: Introductionmentioning

confidence: 99%

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

et al. 2011

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This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.

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“…2͒ was prepared by means of a novel prepolymerization method described in Ref. 29. This method provides irreversible sealing of two PDMS slabs without any need of oxygen plasma or UV/ozone treatment.…”

Section: B Fabrication Of Pdms Chipsmentioning

confidence: 99%

“…31 Reversible microchip sealing mediated by van der Waals interactions was not exploited because the microchip has to withstand high pressure differences. 24 The method 29 used was partially modified and can be shortly described as follows. A phosphore bronze plate 2.5 in.…”

Section: B Fabrication Of Pdms Chipsmentioning

confidence: 99%

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Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties

et al. 2011

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We developed an automated laser induced fluorescence system utilizing microfluidic chips for detection and quantification of immunoglobulins. Microchips were fabricated from polydimethysiloxane ͑PDMS͒ using the so-called "prepolymerization technique." The microchip structure helped minimize the effects of PDMS autofluorescence and light scattering. Furthermore, a thin and uniform PDMS layer forming the top of the microchip enabled proper focusing and collection of the excitation beam and the emitted fluorescence, respectively. The developed system was tested for the detection of mouse immunoglobulins. The capturing antibodies were immobilized on internal microchannel walls in the form of a polyelectrolyte. We clearly show that this immobilization technique, if correctly realized, gives results with high reproducibility. After sample incubation and washing, secondary antibodies labeled by fluorescein isothiocyanate were introduced into microchannels to build a detectable complex. We show that mouse antibodies can be quantified in a wide concentration range, 0.01-100 g ml −1 . The lower detection limit was below 0.001 g ml −1 ͑6.7 pM͒. The developed laser induced fluorescence ͑LIF͒ apparatus is relatively cheap and easy to construct. The total cost of the developed LIF detector is lower than a typical price of plate readers. If compared to classical ELISA ͑enzyme linked immunosorbent assay͒ plate systems, the detection of immunoglobulins or other proteins in the developed PDMS microfluidic device brings other important benefits such as reduced time demands ͑10 min incubation͒ and low reagent consumption ͑less than 1 l͒. The cost of the developed PDMS chips is comparable with the price of commercial ELISA plates. The main troubleshooting related to the apparatus development is also discussed in order to help potential constructors.

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PDMS microfluidic chips prepared by a novel casting and pre-polymerization method

Cited by 23 publications

References 16 publications

Controlling the contents of microdroplets by exploiting the permeability of PDMS

Controlling the contents of microdroplets by exploiting the permeability of PDMS

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

Detection of immunoglobulins in a laser induced fluorescence system utilizing polydimethysiloxane microchips with advanced surface and optical properties

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