PDMS gold nanoparticle composite film-based silver enhanced colorimetric detection of cardiac troponin I

Development of optical biosensor technologies for cardiac troponin recognition

“…They showed that poly(dimethyl siloxane) (PDMS)-gold nanoparticles (AuNPs) composite film as a basis with silver enhancement can be employed by the colorimetric method for detection of cTnI. 59 PDMS has merits such as excellent transparency, outstanding elasticity, good thermal and oxidative stability, ease of fabrication, and the ability to be sealed with various materials. Gold nanoparticles can be functionalized as an idle substrate with antigen, enzyme, and other biomolecules.…”

In situ fabrication of cleavable peptide arrays on polydimethylsiloxane and applications for kinase activity assays

“…Once peeled from the template, the resulting 12  8 array pattern (2.5 mm id and 0.5 mm height for each spot in a space of 5 cm width  4 cm length) of the PDMS substrate was used as the grid for solution printing. The bare PDMS array chip with transferred 12  8 grids was then pipetted with 5 mL HAuCl 4 aqueous solution (1% v/v in DI water) and incubated at room temperature for 48 h to form AuNPs on the surface [20,21].…”

“…As depicted in Steps A-D of Fig. 1, the PDMS substrate was first coated with AuNPs synthesized in situ via the silane redox reaction [20,21], followed by a thiol/amine terminated hetero-PEG and disulfide linker (if required) to result in a free amine functional group. As indicated by the CCD images shown in Fig.…”

Electrogenerated chemiluminescence peptide-based biosensing method for cardiac troponin I using peptide-integrating Ru(bpy)3 2+-functionalized gold nanoparticles as nanoprobe

“…Therefore, it is needed to develop sensitive methods for monitoring cTnI concentration during early stages [3]. Up to now, a lot of methods have been utilized in the determination of cTnI such as colorimetric [4,5], fluorescent [6,7], electroc h e m i c a l [ 8 , 9 ] , c h e m i l u m i n e s c e n c e [ 1 0 ] , a n d electrogenerated chemilminescence (ECL) [11][12][13][14]. Among them, ECL has attracted attention in protein biosensing due to advantages such as a very low background signal, high sensitivity, and good temporal and spatial control [15,16].…”