PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat

Dong, Jie; Zheng, Yong-Xing; Fu, Yihan; Wang, Jinxi; Yuan, Shasha; Wang, Yonghua; Zhu, Qidi; Ou, Xingqi; Li, Gezi

doi:10.1186/s40659-019-0263-2

Cited by 11 publications

(4 citation statements)

References 42 publications

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“…Excessive production of H 2 O 2 can cause leaf cell death. In order to combat reactive oxygen damage, antioxidant enzymes are widely present in plants Systems, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) are ROS scavenging enzymes, which can further convert H 2 O 2 into H 2 O, etc., and play an important role in regulating the content of H 2 O 2 in cells Role [43,44]. In addition, proline is one of the main osmotic adjustment substances in plants.…”

Section: Discussionmentioning

confidence: 99%

Function Analysis of Drought Resistance Related Gene TaGAPCs and TaWRKYs in Wheat

Wang

Zhang

Yang

2021

Preprint

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Backgrounds: Wheat (Triticum aestivum L.) is one of the most important food crops in the world. It faces various abiotic stresses during its growth. Drought is one of the main factors limiting the growth and development of wheat. Severe drought stress will Lead to a decline in wheat production. Cytoplasmic glyceraldehyde-3-phosphate dehydrogenase (GAPC) is an important member of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) family, which is widely present in plant cytoplasm. Plants play an important role in the process of primary metabolism and stress resistance.Result: In this study, a comparative transcriptomic analysis of the TaGAPCs-RNAi strain of Changwu 134 and the wild-type wheat seedlings of Changwu 134 under natural drought conditions was carried out. A total of 30067 differentially expressed genes were screened in RNAi strains and wild-type strains, of which 19,959 genes were up-regulated in RNAi strains and 10,108 genes were down-regulated in transcription. GO analysis shows that differential genes are mainly enriched in biological regulation, cellular processes, metabolic processes, and responses to stimuli. KEGG analysis showed that the differential genes were mainly concentrated in the biosynthesis of phenylpropane, plant hormone signal transduction and flavonoid biosynthesis pathways. By analyzing the expression levels of differential transcription factors, the significantly down-regulated transcription factor WRKY family member TaWRKY2 / 22/28/29/33/40/47/52 in wheat was screened out. The TaWRKY28/33/40/47 gene silencing line was successfully obtained using the barley stripe mosaic virus (BSMV-VIGS) technology. The plants with TaWRKY28/33/40/47 gene silenced were subjected to natural drought treatment, and physiological and biochemical index tests were carried out. The results showed that the growth status of gene-silenced plants was worse than that of wild-type plants, and the relative water content and chlorophyll content decreased. The content of MDA, H2O2 and superoxide anion increases, the activity of antioxidant enzymes (SOD, POD, CAT) decreases, and the content of proline decreases. Conclusion: The results showed that TaGAPCs regulates the expression of some TaWRKYs transcription factors, activates antioxidant pathways, enhances tolerance of wheat to drought stress.

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Section: Discussionmentioning

confidence: 99%

Function Analysis of Drought Resistance Related Gene TaGAPCs and TaWRKYs in Wheat

Wang

Zhang

Yang

2021

Preprint

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“…The barley stripe mosaic virus (BSMV)-based VIGS method was used to create gene knockdown plants ( Ma et al, 2012 ; Dong et al, 2019 ). Briefly, a 283-bp fragment of wheat ATG8 from the conserved coding sequence was amplified and purified, with a same-sized fragment of GFP used as a control.…”

Section: Methodsmentioning

confidence: 99%

Programmed Degradation of Pericarp Cells in Wheat Grains Depends on Autophagy

Yan²,

Cui

et al. 2021

Front. Genet.

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Wheat is one of the most important food crops in the world, with development of the grains directly determining yield and quality. Understanding grain development and the underlying regulatory mechanisms is therefore essential in improving the yield and quality of wheat. In this study, the developmental characteristics of the pericarp was examined in developing wheat grains of the new variety Jimai 70. As a result, pericarp thickness was found to be thinnest in grains at the top of the spike, followed by those in the middle and thickest at the bottom. Moreover, this difference corresponded to the number of cell layers in the pericarp, which decreased as a result of programmed cell death (PCD). A number of autophagy-related genes (ATGs) are involved in the process of PCD in the pericarp, and in this study, an increase in ATG8-PE expression was observed followed by the appearance of autophagy structures. Meanwhile, following interference of the key autophagy gene ATG8, PCD was inhibited and the thickness of the pericarp increased, resulting in small premature grains. These findings suggest that autophagy and PCD coexist in the pericarp during early development of wheat grains, with both processes increasing from the bottom to the top of the spike. Moreover, PCD was also found to rely on ATG8-mediated autophagy. The results of this study therefore provide a theoretical basis for in-depth studies of the regulatory mechanisms of wheat grain development.

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“…Knockdown the expression of TaWRKY24 by using BSMV-VIGS method as our previously published literature described (Gao et al, 2018). One 153 bp fragment (from 175 ~ 249 bp) of TaWRKY24 was cloned and ampli ed into the BSMV-γ vector to form a silencing vector of TaWRKY24 (BSMV-TaWRKY24), and BSMV-GFP was used as a control as our previously described (Dong et al, 2019). After inoculation for 8 ~ 10 days, the inoculated wheat seedlings were observed obvious phenotype, and its leaves were collected for RNA extraction to measure the gene expression levels.…”

Section: Bsmv-vigs-mediated Gene Silencingmentioning

confidence: 99%

Wheat WRKY24-1D, interacts with TaERFL1a, regualtes DHAR-mediated ASA-GSH biosynthesis to enhance drought tolerance in wheat

Li,

Liu,

Wang

et al. 2024

Preprint

Self Cite

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Drought is a major environmental factor to limit wheat yield and quality. WRKY transcription factors play crucial roles in abiotic stresses, including drought stress. However, the regulatory mechanisms of WRKYs in wheat drought stress tolerance are largely unknown. In this study, we found a WRKY transcription factors TaWRKY24, which identified and screened between drought stress yeast library and drought-induced gene TaERFL1a, and the other yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) found that TaERFL1a was interacted with TaWRKY24-1D, which was more induced than TaWRKY24-1A under drought stress. Moreover, the potential function of TaWRKY24 was examined by using BSMV-VIGS method. Results found that transiently silenced of TaWRKY24 in wheat resulted in enhanced sensitivity to drought stress by increasing the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), decreasing the activities of ascorbate peroxidase (APX), superoxide dismutase (SOD), or catalase (CAT), and contents of ascorbate (ASA) and glutathione (GSH), and inhibiting the biomass and relative water content. qPCR showed that the expression levels of ASA-GSH-related genes were also significantly inhibited in TaWRKY24-silenced wheat plants. Among these genes, dehydroascorbate reductase (DHAR) had a lowest expression than other ASA-GSH-related genes, indicating that it expression may be mainly regulate by TaWRKY24. In addition, yeast one-hybrid (Y1H), luciferase complementation imaging (LCI), and luciferase (LUC) assays showed that TaWRKY24 had higher activity to bound the promoter of DHAR than TaERFL1a, suggesting that TaWRKY24 positively regulated the expression levels of DHAR and interacted with TaERFL1a to involve in wheat drought tolerance. Therefore, these results providing a theoretical basis for the molecular regulatory mechanisms of TaWRKY24 in wheat drought resistance, and contributing to the potential candidate genes for breeding the drought resistance wheat varieties.

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PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat

Cited by 11 publications

References 42 publications

Function Analysis of Drought Resistance Related Gene TaGAPCs and TaWRKYs in Wheat

Function Analysis of Drought Resistance Related Gene TaGAPCs and TaWRKYs in Wheat

Programmed Degradation of Pericarp Cells in Wheat Grains Depends on Autophagy

Wheat WRKY24-1D, interacts with TaERFL1a, regualtes DHAR-mediated ASA-GSH biosynthesis to enhance drought tolerance in wheat

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