PDGFRα controls the balance of stromal and adipogenic cells during adipose tissue organogenesis

Sun, Chengyi; Berry, William L.; Olson, Lorin E.

doi:10.1242/dev.135962

Cited by 70 publications

(64 citation statements)

References 54 publications

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“…These cells, which are positive for platelet-derived growth factor receptor  or , seem to derive from a common progenitor as pre-adipocytes destined to mature into adipocytes. These cells therefore seem to be at the crossroad between healthy and unhealthy adipose tissue development (65,66). Despite the fact that the adiponectin promotor has become the preferred tool for generating adipocyte-specific knockout mice, the aP2-driven model applied in this study has the advantage that it also targets the adipocyte precursors.…”

Section: Discussionmentioning

confidence: 99%

FXR overexpression alters adipose tissue architecture in mice and limits its storage capacity leading to metabolic derangements

Zutphen

Stroeve

Yang

et al. 2019

Journal of Lipid Research

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Section: Discussionmentioning

confidence: 99%

FXR overexpression alters adipose tissue architecture in mice and limits its storage capacity leading to metabolic derangements

Zutphen

Stroeve

Yang

et al. 2019

Journal of Lipid Research

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“…Other signaling molecules that are known to regulate adipogenesis, such as PdgfRa, insulin receptor, and Igf1R, localize to the ciliary membrane upon growth arrest and ciliogenesis/ciliary elongation (25,26,29,(66)(67)(68). There is extensive crosstalk among pathways that are mediated by these molecules (69) and evidence that Rpgrip1l may participate in some of this coordination (70,71).…”

Section: Discussionmentioning

confidence: 99%

The role of Rpgrip1l, a component of the primary cilium, in adipocyte development and function

et al. 2018

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Genetic variants within the FTO (α-ketoglutarate-dependent dioxygenase) gene have been strongly associated with a modest increase in adiposity as a result of increased food intake. These risk alleles are associated with decreased expression of both FTO and neighboring RPGRIP1L (retinitis pigmentosa GTPase regulator-interacting protein 1 like). RPGRIP1L encodes a protein that is critical to the function of the primary cilium, which conveys extracellular information to the cell. Rpgrip1l mice exhibit increased adiposity, in part, as a result of hyperphagia. Here, we describe the effects of Rpgrip1l in adipocytes that may contribute to the adiposity phenotype observed in these animals and possibly in humans who segregate for FTO risk alleles. Loss of Rpgrip1l in 3T3-L1 preadipocytes increased the number of cells that are capable of differentiating into mature adipocytes. Knockout of Rpgrip1l in mature adipocytes using Adipoq-Cre did not increase adiposity in mice that were fed chow or a high-fat diet. We also did not observe any effects of Rpgrip1l knockdown in mature 3T3-L1 adipocytes. Thus, to the extent that Rpgrip1l affects cell-autonomous adipose tissue function, it may do so as a result of the effects conveyed in preadipocytes in which the primary cilium is functionally important. We propose that decreased RPGRIP1L expression in preadipocytes in humans who segregate for FTO obesity risk alleles may increase the storage capacity of adipose tissue.-Martin Carli, J. F., LeDuc, C. A., Zhang, Y., Stratigopoulos, G., Leibel, R. L. The role of Rpgrip1l, a component of the primary cilium, in adipocyte development and function.

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“…Within mouse VIS (perigonadal) adipose depot, TCF21 protein was seen exclusively in cells positive for platelet-derived growth factor receptor ␣, a cell-surface marker of fibroblastadipocyte progenitors (Fig. 1F) (19,20). On the contrary, TCF21 was undetectable in SQ (inguinal) depots isolated from the same animals ( Fig.…”

Section: Tcf21 Is Co-expressed With Il6 and Specific Ecm Remodeling Fmentioning

confidence: 95%

Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells

Akama

Chun

2018

Journal of Biological Chemistry

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The visceral (VIS) and subcutaneous (SQ) fat pads are developmentally distinct white adipose tissue depots and contribute differently to inflammation and insulin resistance associated with obesity. The basic helix-loop-helix transcriptional regulator, transcription factor 21 (TCF21), is a marker gene for white adipose tissues and is abundantly expressed in VIS-derived adipose stem cells (ASCs), but not in SQ-derived ASCs. However, TCF21's role in regulating fat depot-specific gene expression and function is incompletely understood. Here, using siRNA-mediated knockdowns and lentiviral gene transfer of in mouse ASCs, we demonstrate that TCF21 is required for the VIS ASC-specific expression of interleukin 6 (IL6), a key cytokine that contributes to the proinflammatory nature of VIS depots. Concurrently, TCF21 promotes MMP-dependent collagen degradation and type IV collagen deposition through the regulation of the extracellular matrix (ECM) modifiers, matrix metalloproteinase (MMP) 2, MMP13, and tissue inhibitor of MMP1 (TIMP1), as well as collagen type IV α1 chain (COL4A1) in VIS ASCs. We also found that although IL6 mediates the expression of and in VIS ASCs, the TCF21-dependent expression of and is IL6-independent. These results suggest that TCF21 contributes to the proinflammatory environment in VIS fat depots and to active ECM remodeling of these depots by regulating IL6 expression and MMP-dependent ECM remodeling in a spatiotemporally coordinated manner.

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PDGFRα controls the balance of stromal and adipogenic cells during adipose tissue organogenesis

Cited by 70 publications

References 54 publications

FXR overexpression alters adipose tissue architecture in mice and limits its storage capacity leading to metabolic derangements

FXR overexpression alters adipose tissue architecture in mice and limits its storage capacity leading to metabolic derangements

The role of Rpgrip1l, a component of the primary cilium, in adipocyte development and function

Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells

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