PdEPFL6 reduces stomatal density to improve drought tolerance in poplar

Jiao, Zhiyin; Han, Shuo; Li, Zheng; Huang, Mengbo; Niu, Meng-Xue; Yu, Xiao; Liu, Chao; Wang, Hou‐Ling; Yin, Weilun; Xia, Xinli

doi:10.1016/j.indcrop.2022.114873

Cited by 19 publications

(18 citation statements)

References 83 publications

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“…On the one hand, considering the increased wax accumulation in alfalfa OE plants (Figure 9g), this phenomenon could be explained by the fact that in alfalfa OE plants, epicuticular wax can maintain the water potential relatively high by reducing the leaf transpiration rate, which might be helpful in maintaining high levels of photosynthesis (Jiang et al, 2009). On the other hand, our stomatal and photosynthetic data are in agreement with data from other transgenic plants, such as Populus expressing PeCHYR1 (He et al, 2018), PdGNC (Shen et al, 2021), or PdEPFL6 (Jiao et al, 2022) and maize expressing ZmSDD1 (Liu et al, 2015). They suggested that photosynthesis is more affected by metabolic impairment in the photosynthetic apparatus than by stomatal factors (He et al, 2018;Shen et al, 2021).…”

Section: Discussionsupporting

confidence: 89%

NUCLEAR TRANSPORT FACTOR 2‐LIKE improves drought tolerance by modulating leaf water loss in alfalfa (Medicago sativa L.)

Luo

Liu

et al. 2022

The Plant Journal

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Drought is a major environmental factor that limits the production of alfalfa (Medicago sativa). In the present study, M. sativa NUCLEAR TRANSPORT FACTOR 2-LIKE (MsNTF2L) was identified as a nucleus-, cytoplasm-, and plasma membrane-localized protein. Its transcriptional expression was highly induced by ABA and drought stress. Overexpression of MsNTF2L in Arabidopsis resulted in hypersensitivity to ABA during both the seed germination and seedling growth stages. However, transgenic Arabidopsis plants exhibited enhanced tolerance to drought stress by reducing the levels of reactive oxygen species (ROS) and increasing the expression of stress/ABA-inducible genes. Consistently, analysis of MsNTF2L overexpression (OE) and RNA interference (RNAi) alfalfa plants revealed that MsNTF2L confers drought tolerance through promoting ROS scavenging, a decrease in stomatal density, ABA-induced stomatal closure, and epicuticular wax crystal accumulation. MsNTF2L highly affected epicuticular wax deposition, as a large group of wax biosynthesis and transport genes were influenced in the alfalfa OE and RNAi lines. Furthermore, transcript profiling of drought-treated alfalfa WT, OE, and RNAi plants showed a differential drought response for genes related to stress/ABA signaling, antioxidant defense, and photosynthesis. Taken together, these results reveal that MsNTF2L confers drought tolerance in alfalfa via modulation of leaf water loss (by regulating both stomata and wax deposition), antioxidant defense, and photosynthesis.

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Section: Discussionsupporting

confidence: 89%

NUCLEAR TRANSPORT FACTOR 2‐LIKE improves drought tolerance by modulating leaf water loss in alfalfa (Medicago sativa L.)

Luo

Liu

et al. 2022

The Plant Journal

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“…It was found that the overexpression of PdEPFL6 regulated the expression levels of MAPK-associated genes and rapidly decreased the expression of the transcription factors related to stomatal development (such as PdSPCH and PdMUTE ) under drought conditions, consequently leading to a reduction in stomatal density. This resulted in a marked improvement in drought tolerance in poplar by limiting transpirational water loss [ 76 ]. Plants with a reduced expression of SDD1 and TMM1 have increased their stomatal density with a low level of stomatal pairing.…”

Section: Molecular Regulation Of Stomatal Density For Osmotic Stress ...mentioning

confidence: 99%

How Does Stomatal Density and Residual Transpiration Contribute to Osmotic Stress Tolerance?

Hasanuzzaman

Zhou

Shabala

2023

Plants

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Osmotic stress that is induced by salinity and drought affects plant growth and development, resulting in significant losses to global crop production. Consequently, there is a strong need to develop stress-tolerant crops with a higher water use efficiency through breeding programs. Water use efficiency could be improved by decreasing stomatal transpiration without causing a reduction in CO2 uptake under osmotic stress conditions. The genetic manipulation of stomatal density could be one of the most promising strategies for breeders to achieve this goal. On the other hand, a substantial amount of water loss occurs across the cuticle without any contribution to carbon gain when the stomata are closed and under osmotic stress. The minimization of cuticular (otherwise known as residual) transpiration also determines the fitness and survival capacity of the plant under the conditions of a water deficit. The deposition of cuticular wax on the leaf epidermis acts as a limiting barrier for residual transpiration. However, the causal relationship between the frequency of stomatal density and plant osmotic stress tolerance and the link between residual transpiration and cuticular wax is not always straightforward, with controversial reports available in the literature. In this review, we focus on these controversies and explore the potential physiological and molecular aspects of controlling stomatal and residual transpiration water loss for improving water use efficiency under osmotic stress conditions via a comparative analysis of the performance of domesticated crops and their wild relatives.

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“…The set of genes isolated from the high-WUE P. nigra × ( P.deltoides × P.nigra ) hybrid NE-19 included PdERECTA and the signal peptide-encoding PdEPFL6 [ 57 , 58 , 59 ], both negative regulators of stomata differentiation. Overexpression of PdERECTA and PdEPFL6 also resulted in decreased stomatal density and increased drought tolerance without a significant reduction in biomass accumulation under non-limiting water conditions.…”

Section: Identification Of Genes and Pathways Modulating Stomatal Dev...mentioning

confidence: 99%

“…Overall, published works suggest that reduction of stomatal density is a promising strategy for breeding or biotechnological programs aimed at obtaining drought resilient poplar genotypes [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. However, field-based trials remain desirable, as some observations made in natural conditions are not fully in agreement with those in confined environments.…”

Section: Identification Of Genes and Pathways Modulating Stomatal Dev...mentioning

confidence: 99%

Responses to Drought Stress in Poplar: What Do We Know and What Can We Learn?

Rosso¹,

Cantamessa²,

Bergante³

et al. 2023

Life

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Poplar (Populus spp.) is a high-value crop for wood and biomass production and a model organism for tree physiology and genomics. The early release, in 2006, of the complete genome sequence of P. trichocarpa was followed by a wealth of studies that significantly enriched our knowledge of complex pathways inherent to woody plants, such as lignin biosynthesis and secondary cell wall deposition. Recently, in the attempt to cope with the challenges posed by ongoing climate change, fundamental studies and breeding programs with poplar have gradually shifted their focus to address the responses to abiotic stresses, particularly drought. Taking advantage from a set of modern genomic and phenotyping tools, these studies are now shedding light on important processes, including embolism formation (the entry and expansion of air bubbles in the xylem) and repair, the impact of drought stress on biomass yield and quality, and the long-term effects of drought events. In this review, we summarize the status of the research on the molecular bases of the responses to drought in poplar. We highlight how this knowledge can be exploited to select more tolerant genotypes and how it can be translated to other tree species to improve our understanding of forest dynamics under rapidly changing environmental conditions.

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PdEPFL6 reduces stomatal density to improve drought tolerance in poplar

Cited by 19 publications

References 83 publications

NUCLEAR TRANSPORT FACTOR 2‐LIKE improves drought tolerance by modulating leaf water loss in alfalfa (Medicago sativa L.)

NUCLEAR TRANSPORT FACTOR 2‐LIKE improves drought tolerance by modulating leaf water loss in alfalfa (Medicago sativa L.)

How Does Stomatal Density and Residual Transpiration Contribute to Osmotic Stress Tolerance?

Responses to Drought Stress in Poplar: What Do We Know and What Can We Learn?

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