PD‐L1, B7‐H3 and VISTA are highly expressed in gestational trophoblastic neoplasia

Zong, Liju; Zhang, Ming; Wang, Wenze; Wan, Xirun; Yang, Junjun; Xiang, Yang

doi:10.1111/his.13882

Cited by 50 publications

(46 citation statements)

References 30 publications

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“…Expression of PD-L1, B7-H3, B7-H4, and VISTA in CCC of the Cervix. Consistent with our previous study [13,14], the patterns of PD-L1, B7-H3, B7-H4, and VISTA staining in cervical CCC were predominantly membranous; representative images are shown Figure 1. PD-L1 was expressed in both TCs and TAICs and was positive in 11 samples (22%).…”

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confidence: 90%

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Expression and Significance of Immune Checkpoints in Clear Cell Carcinoma of the Uterine Cervix

Zong

Zhang

Zhou

et al. 2020

Journal of Immunology Research

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The purpose of this study was to investigate the expression levels of the immune checkpoint proteins, programmed cell death-ligand 1 (PD-L1), B7-H3, B7-H4, and V-domain Ig suppressor of T cell activation (VISTA), as well as the significance thereof, in clear cell carcinoma (CCC) of the cervix (a rare histological subtype of cervical cancer). We also compared the expression statuses of these biomarkers in cervical CCCs with those in cervical squamous cell carcinomas (SCCs). We evaluated the expression of PD-L1, B7-H3, B7-H4, and VISTA in 50 cervical CCCs and 100 SCCs using immunohistochemical staining and investigated the associations between these markers, clinicopathologic features, and survival in patients with CCCs. Of the cervical CCC samples examined, 22%, 16%, 32%, and 34% were positive for PD-L1, B7-H3, B7-H4, and VISTA, respectively. Nineteen samples (38%) were negative for all 4 of these markers, whereas 31 (62%) expressed at least 1 marker. None of these markers was associated with the investigated clinicopathologic variables or patient survival. PD-L1, B7-H3, and VISTA were observed significantly more frequently in SCCs than in CCCs of the cervix. Our study confirmed the expression of immune checkpoint proteins in cervical CCCs and indicated their nonredundant and complementary roles. As such, our data suggest that monotherapeutic immune checkpoint blockade may not be sufficiently effective in patients with cervical CCC.

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confidence: 90%

“…Immunohistochemistry (IHC) was performed using our laboratory protocol as described previously [13,14]. Briefly, 4 μm series of whole tissue sections were deparaffinized and subjected to heat-induced epitope retrieval with 10 mM sodium citrate (pH 6.0) at 95°C for 20 minutes.…”

Section: Immunohistochemistry and Evaluationmentioning

confidence: 99%

Expression and Significance of Immune Checkpoints in Clear Cell Carcinoma of the Uterine Cervix

Zong

Zhang

Zhou

et al. 2020

Journal of Immunology Research

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“…Using real-time polymerase chain reaction, Lines et al found that VISTA was predominantly expressed in human placental and hematopoietic tissues, as well as in tissues that exhibit markedly high leukocyte infiltration [28]. We also found that normal human organs other than lymphoid tissues and placental trophoblastic cells did not express VISTA at the protein level [13]. Although VISTA protein has been detected in gastric cancer, oral squamous cell carcinoma, NSCLC, colorectal carcinoma, hepatocellular carcinoma, endometrial cancer, ovarian cancer, melanoma, and gestational trophoblastic neoplasia, its expression and relationship with patient survival vary according to the type of cancer [13][14][15][16][17][18][19][20][21].…”

Section: Discussionsupporting

confidence: 53%

“…Human tonsil and placenta tissues that were obtained from the Department of Pathology, Peking Union Medical College Hospital, Beijing, China, were used as positive controls, whereas normal tonsil tissues without primary antibodies were used as negative controls. Immunohistochemistry was performed using our laboratory protocol as described previously [13,22].…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…These studies suggest that VISTA may modulate a novel immune evasion mechanism and is thus a potential target for cancer immunotherapy. VISTA expression in human cancers has been reported in non-small cell lung cancer (NSCLC), hepatocellular carcinoma, colorectal carcinoma, oral squamous cell carcinoma, gastric carcinoma, acute myeloid leukemia, and gestational trophoblastic neoplasia [13][14][15][16][17][18][19][20][21]. Mulati et al also found that VISTA was highly expressed in human ovarian and endometrial cancers [20].…”

Section: Introductionmentioning

confidence: 98%

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VISTA expression is associated with a favorable prognosis in patients with high-grade serous ovarian cancer

Zong

Zhou

Zhang

et al. 2019

Cancer Immunol Immunother

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Blockading programmed death ligand 1 (PD-L1) shows promising results in patients with some cancers, but not in those with ovarian cancer. V-domain Ig suppressor of T cell activation (VISTA) is a recently discovered immune checkpoint protein that suppresses T cell activation. This study aimed to investigate the expression and clinical significance of VISTA in ovarian cancer as well as its relationship with PD-L1. VISTA and PD-L1 levels in 146 ovarian cancer samples were assessed using immunohistochemistry. We investigated the association between VISTA and other clinicopathological variables, including survival. The associations between the VISTA-encoding C10orf54 gene, other immune checkpoints, and survival were analyzed. VISTA was detected in 51.4% of all samples and 46.6% of PD-L1-negative samples; it was expressed in 28.8%, 35.6%, and 4.1% of tumor cells (TCs), immune cells (ICs), and endothelial cells, respectively. Furthermore, VISTA expression was associated with pathologic type and PD-L1 expression. Moreover, VISTA expression in TCs, but not in ICs, was associated with prolonged progression-free and overall survival in patients with high-grade serous ovarian cancer. The expression of C10orf54 mRNA was associated with prolonged overall survival and immune escape-modulating genes. These results showed that VISTA expression in ovarian tumor cells was associated with a favorable prognosis in patients with high-grade serous ovarian cancer; however, additional studies are required to better understand the expression and role of VISTA in ovarian cancer. Keywords Ovarian cancer • PD-L1 • VISTA • Immune checkpoints • Prognosis Abbreviations CTLA4 Cytotoxic T-lymphocyte-associated protein 4 FIGO International federation of gynecology and obstetrics HGSOC High-grade serous ovarian carcinoma IC Immune cell LAG3 Lymphocyte activation gene-3 TCGA The cancer genome atlas TC Tumor cell TIGIT T cell immunoreceptor with Ig and ITIM domains TIM-3 T cell immunoglobulin and mucin domain-3 TMA Tumor tissue microarray VISTA V-domain Ig suppressor of T cell activation Electronic supplementary material The online version of this article (

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Dominant immune cells in pregnancy and pregnancy complications: T helper cells (TH1/TH2, TH17/Treg cells), NK cells, MDSCs, and the immune checkpoints

Esparvarinha

Madadi

Aslanian‐Kalkhoran

et al. 2022

Cell Biology International

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Pregnancy problems including recurrent pregnancy loss, repeated implantation failure and pre-eclampsia are common problems in the reproductive ages. Different reasons such as genetic, immunological, and environmental agents and also infections could develop these complications. In those cases in which the cause of the abortion is diagnosed, the chance of a successful pregnancy is increased by eliminating defective factors. However, in patients with unknown causes, there may be an imbalance in immune cells pattern. As a matter of fact, an inappropriate immune response is often associated with a failed pregnancy. Hence, the focus of treatment is to increase tolerance, not to suppress maternal immune system. These findings are linked to an elevated number of Treg cells and immune checkpoints through normal pregnancy. The present review discusses the balance of myeloidderived suppressor cells, natural killer cells, T cells, and immune checkpoints, and also targeting them to maintain pregnancy and prevent associated complications.

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PD‐L1, B7‐H3 and VISTA are highly expressed in gestational trophoblastic neoplasia

Cited by 50 publications

References 30 publications

Expression and Significance of Immune Checkpoints in Clear Cell Carcinoma of the Uterine Cervix

Expression and Significance of Immune Checkpoints in Clear Cell Carcinoma of the Uterine Cervix

VISTA expression is associated with a favorable prognosis in patients with high-grade serous ovarian cancer

Dominant immune cells in pregnancy and pregnancy complications: T helper cells (TH1/TH2, TH17/Treg cells), NK cells, MDSCs, and the immune checkpoints

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