PCR survey of vectorborne pathogens in dogs living in and around Barcelona, an area endemic for leishmaniosis

Tabar, María‐Dolores; Francino, Olga; Altet, Laura; Sánchez, Armand; Ferrer, L.; Roura, Xavier

doi:10.1136/vr.164.4.112

Cited by 67 publications

(56 citation statements)

References 45 publications

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“…This study focused on Babesia and Theileria species/types, but other pathogens may also infect both horses and dogs, such as Anaplasma Ehrlichia, Rickettisia/Neorickettsia, Borrelia, Bartonella, Mycoplasma haemocanis, etc. (Tabar et al 2009) and worsen the clinical course. Association with Leptospira interrogans is also frequent and under-diagnosed specially in horses.…”

Section: Discussionmentioning

confidence: 95%

A PCR study of piroplasms in 166 dogs and 111 horses in France (March 2006 to March 2008)

Fritz¹

2010

Parasitol Res

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Positive PCR results for piroplasms were obtained on EDTA blood samples from 166 dogs and 111 horses between March 2006 and March 2008. The organisms were initially identified using common primers, followed by restriction enzyme profiles to determine the species and types of Babesia/Theileria. In 27 dogs and eight horses with positive results, the size of the specimen was insufficient to separate species/types. We identified Babesia canis canis in 105 of the 166 dogs (63%) and Theileria equi in 89 of the 111 horses (80%). Babesia caballi was also present, but rare, in only two Babesia/Theileria of the 111 horses (1.2%). Unexpected findings were the identification of T. equi in 31 of the 166 dogs (19%), of B. caballi in one of the 166 dogs (0.6%), of B. canis canis in 11 of 111 horses (10%), as well as of Babesia canis rossi in one of 111 horses (0.9%). A positive result for B. canis rossi was also obtained in one of the 166 dogs (0.6%). A single case of the California type of "Babesia gibsoni" (presumably Babesia conradae) was found in a dog. We did not detect Babesia canis vogeli, possibly because of the geographic distribution of our clients. The unusual results were confirmed by DNA sequencing in several cases for B. canis canis and B. canis rossi in horses, as well as T. equi in dogs. Such infections might explain the difficulties met in some unsuccessfully treated animals using the standard dosage of imidocarb dipropionate at 3 mg/kg in dogs.

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Section: Discussionmentioning

confidence: 95%

A PCR study of piroplasms in 166 dogs and 111 horses in France (March 2006 to March 2008)

Fritz¹

2010

Parasitol Res

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“…Several studies have detected this species in dogs from Hungary, Croatia, Italy, Poland, Spain, Switzerland, Portugal, Russia, Germany, Slovenia, France, Albania, the Netherlands, Norway (Solano-Gallego and Baneth, 2011) and Romania (Ionita et al, 2012). Babesia gibsoni infection has been described in some European countries using molecular methods: two dogs in Germany (Hartelt et al, 2007), four in Spain (Criado-Fornelio et al, 2003;Tabar et al, 2009), one dog in Italy (Trotta et al, 2009), 14 dogs in Romania (Imre et al, 2013) and six from Croatia were diagnosed with B. gibsoni subclinical infection (Beck et al, 2009). Our results support previous findings reported from neighbouring countries that B. canis is considered the main species which causes canine babesiosis in this part of Europe.…”

Section: Discussionmentioning

confidence: 99%

Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia

Davitkov

Vučićević

Stevanović

et al. 2015

Acta Veterinaria Hungarica

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Canine babesiosis is a frequent and clinically significant tick-borne disease. Sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in Serbia. After clinical examination, blood samples were taken for microscopic examination, complete blood count (CBC), Canine SNAP 4Dx Test, DNA analyses and sequencing. The main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, and vomiting. The main clinicopathological findings in Babesia infections were a slight to severe thrombocytopenia and a mild to very severe normocytic normochromic anaemia. Microscopic evaluation revealed 58 positive samples with the presence of large and small intraerythrocytic piroplasms in 57 and 1 sample(s), respectively. No co-infections were found using SNAP test. Two Babesia species, B. canis (58/60) and B. gibsoni (2/60), were differentiated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Species identification was further confirmed by sequencing PCR products of B. gibsoni samples and six randomly selected B. canis samples. All dogs were treated with imidocarb dipropionate (6.6 mg/kg of body weight), given intramuscularly twice at an interval of 14 days. This report presents the first molecular evidence of the occurrence of B. gibsoni and B. canis, confirmed by DNA sequencing, in sick dogs from Serbia.

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“…Clinical cases of B. gibsoni infection have been reported in Spain [69,70] Germany [71], Croatia [23], Italy [72] and Serbia [73], but also in other parts of the world such as Asia, United States, Australia and Brasil [60,74,75]. It is known that in some parts of the world Babesia gibsoni can be transmitted by dog bites during the fi ghting.…”

Section: Taxonomy and Geographic Distributionmentioning

confidence: 99%

Canine Babesiosis: Where Do We Stand?

et al. 2018

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Canine babesiosis is a tick-borne disease caused by protozoal haemoparasites of different Babesia species. Babesiosis is one of the most important globally extended and quickly spreading tick-borne infections of dogs. This comprehensive review gives an in-depth overview of Babesia species currently identifi ed in dogs together with relevant vector tick species and their geographical distribution, life cycle and transmission of parasite. The main mechanisms in the pathogenesis of babesiosis are described and elucidated by recent literature overview. As Babesia infection causes a disease with very variable clinical manifestations, special attention is given to clinical signs, laboratory features and clinicopathological fi ndings. The diagnosis of canine babesiosis by microscopy, serological and molecular methods is reviewed, together with recent advances in mass spectrometry based assays. Accurate detection and species recognition are important for the selection of the appropriate therapy, monitoring and prediction of the outcome of the disease. Finally, guidelines for the treatment and prevention of canine babesiosis are given.

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PCR survey of vectorborne pathogens in dogs living in and around Barcelona, an area endemic for leishmaniosis

Cited by 67 publications

References 45 publications

A PCR study of piroplasms in 166 dogs and 111 horses in France (March 2006 to March 2008)

A PCR study of piroplasms in 166 dogs and 111 horses in France (March 2006 to March 2008)

Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia

Canine Babesiosis: Where Do We Stand?

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