PCR-SSCP and Sequencing of CXCR1 (IL8RA) Gene in Indian Water Buffalo

Abstract

Genetic markers associated with inflammatory responses could help in selecting the animals susceptible/tolerant to mastitis. The selective breeding assisted by these markers could help to reduce the huge economic losses that are posed by various forms of mastitis. One possible marker is CXCR1, a chemokine receptor that is required for neutrophil migration to infection site. Therefore the present study was planned to identify genetic polymorphism (if any) in CXCR1 gene and associate it with subclinical mastitis in Riverine buffalo of Northern India. For this, two hundred healthy lactating water buffalo were randomly chosen from the herds maintained by various farmers in Kapurthala District of Punjab, India. Blood and milk samples of selected buffaloes were collected. Screening of the animals for sub clinical mastitis was done by SCC and CMT assays of milk samples. Genomic DNA was isolated from blood samples by phenol chloroform method. The DNA of good quality was used for further analysis. PCR-SSCP was used to explore the polymorphism in 311 bp fragment of partial exon2 of CXCR1 gene. The 311 bp fragment of CXCR1 gene was found to be monomorphic in all the DNA samples screened of Indian water buffalo.

Antimicrobial Resistance Profile and Molecular Characterization of Staphylococcus aureus Isolated from Subclinical Mastitis of Dairy Cows in Mathura Region

“…Prevalence of subclinical mastitis in dairy animals caused by S. aureus has been reported in recent years from developing countries including Iran (Beheshti et al, 2011), Brazil (Busanello et al, 2017), Ethiopia (Birhanu et al, 2017;Tegegne et al 2021), Egypt (Algammal et al, 2020;Moustafa et al 2021) and Pakistan (Javed et al, 2021). In India, subclinical mastitis in cattle and buffaloes has been reported in different states such as Karnataka, Punjab and others (Hegde et al, 2013;Kaur et al, 2015;Das et al, 2017).…”