PCR Screening in the Routine of Blood Banking of the German Red Cross Blood Transfusion Service of Baden-Würtemberg

Cardoso, M. da Silva; Koerner, K.; Kubanek, B

doi:10.1159/000053406

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…Entrapment of HCV in the clots and the inhibitory effects of heparin on the PCR have also been evaluated [4,13]. These observations are not in accordance with recent reports of Miskowsky et al [14], Damen et al [13] and Da Silva-Cardoso et al [5,6]. Miskowsky et al could not ®nd any difference in the concentration of circulating HCV RNA between samples collected in plasma tubes (EDTA or ACD) or serotubes [14].…”

Section: Discussionmentioning

confidence: 90%

“…Da Silva-Cardoso et al reported that after dilution and long-term storage (21 days at +4°C), the presence of HCV RNA was still detectable by using a sensitive qualitative test [5]. In a further study, these authors suggest that the presence of blood cells and the type of sample (serum or plasma) have only a small effect on HCV RNA concentration after storage at +4°C for 168 h [6]. No data are available concerning the effects of multiple cycles of freezing and thawing.…”

Section: Discussionmentioning

confidence: 91%

“…Moreover, we considered, as signi®cant, an HCV RNA titre variation of almost 0.5 log. This criteria is more restrictive than that adopted by other authors [5,6].…”

Section: Discussionmentioning

confidence: 94%

“…However, immediate whole blood separation into plasma and packed red cells and sophisticated storage conditions are not always practicable for the blood transfusion service with mobile and/or decentralized blood collection units. More recently, some authors reported that HCV RNA is relatively stable in EDTA-collected whole blood samples which are kept at +4°C [5,6]. At present there are no data concerning the stability of HCV RNA after repeated cycles of freezing and thawing.…”

Section: Introductionmentioning

confidence: 99%

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The stability of hepatitis C virus RNA after storage at +4 °C

Gessoni¹,

Barin²,

Frigato³

et al. 2000

Journal of Viral Hepatitis

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One of the major issues in nucleic acid testing is how to store blood samples to obtain reliable results. We therefore studied hepatitis C virus (HCV) RNA concentration in samples after storage at +4 degrees C or freezing and thawing. Six HCV RNA-positive, untreated subjects were studied. Blood samples were collected from these subjects in plasma preparation tubes. The HCV RNA concentration was analysed after storage at +4 degrees C for 168 h or after five freeze-thaw cycles. For HCV RNA quantification we used a qualitative and a quantitative commercial test. After 168 h of storage at +4 degrees C, the HCV RNA concentration was similar to that observed at time-point 0 (5.025 log vs 5.492 log). In one sample we observed a significant fall in HCV RNA concentration. After five freeze-thaw cycles, the HCV RNA concentration was lower than that observed at time-point 0 (4.454 log vs 5.492 log) and in four samples we observed a significant fall in HCV RNA concentration. Our data suggest that HCV RNA is stable in whole blood samples stored at +4 degrees C for 168 h. Based on our results, we conclude that the standard procedures for transport of blood samples (at room temperature for a maximum of 5 h) and storage schedules (+4 degrees C for a maximum of 48 h) can be maintained without compromising the quality of results.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 91%

“…Moreover, we considered, as signi®cant, an HCV RNA titre variation of almost 0.5 log. This criteria is more restrictive than that adopted by other authors [5,6].…”

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The stability of hepatitis C virus RNA after storage at +4 °C

Gessoni¹,

Barin²,

Frigato³

et al. 2000

Journal of Viral Hepatitis

View full text Add to dashboard Cite

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“…Furthermore, it has to be noted that our validation was performed exclusively with plasma samples, and results cannot be transferred directly to serum samples. Interestingly, a study by Da Silva Cardoso et al [22] suggests that the presence of blood cells and the type of blood sample (serum or plasma) has only a small effect on the HCV RNA concentration after storage at 4 ° C.…”

Section: Discussionmentioning

confidence: 99%

HCV RNA Testing of Plasma Samples from Cornea Donors: Suitability of Plasma Samples Stored at 4 °C for up to 8 Days

Berger

Salla

Keppler

et al. 2016

Transfus Med Hemother

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Background: The HCV RNA testing of potential cornea donors frequently relies on blood samples stored pre mortem. The recommended storage time of maximum 72 h frequently excludes a significant fraction of donors. Methods: The influence of storage time of EDTA plasma samples at 4 °C on the viral load measured with the Roche HCV Quantitative Test vs. 2.0 was evaluated for 43 samples from HCV-positive individuals. Results: The mean reduction of the viral load after 4 °C storage for 6-8 days was 0.46 log₁₀ IU/ml (range +0.17 to -1.66 log₁₀ IU/ml). After 1-3 days a mean loss of 0.19 log₁₀ IU/ml (range +0.30 to -1.41 log₁₀ IU/ml) and after 3-5 days of 0.32 log₁₀ IU/ml (range +0.36 to -1.81 log₁₀ IU/ml) was observed. In 23.3% of samples, a viral load reduction ≥ 1 log₁₀ IU/ml (1.0-1.81 log₁₀ IU/ml) was found after prolonged storage (5-8 days). In none of the samples did the HCV load fall below the detection limit. Conclusion: Plasma storage for up to 8 days can quantitatively reduce the HCV RNA load, yet has no influence on the reliability of a qualitative HCV RNA detection by this ultrasensitive test to determine the HCV status of serologically negative cornea donors.

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