1998
DOI: 10.1159/000053406
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PCR Screening in the Routine of Blood Banking of the German Red Cross Blood Transfusion Service of Baden-Würtemberg

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Cited by 9 publications
(6 citation statements)
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“…Entrapment of HCV in the clots and the inhibitory effects of heparin on the PCR have also been evaluated [4,13]. These observations are not in accordance with recent reports of Miskowsky et al [14], Damen et al [13] and Da Silva-Cardoso et al [5,6]. Miskowsky et al could not ®nd any difference in the concentration of circulating HCV RNA between samples collected in plasma tubes (EDTA or ACD) or serotubes [14].…”
Section: Discussionmentioning
confidence: 90%
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“…Entrapment of HCV in the clots and the inhibitory effects of heparin on the PCR have also been evaluated [4,13]. These observations are not in accordance with recent reports of Miskowsky et al [14], Damen et al [13] and Da Silva-Cardoso et al [5,6]. Miskowsky et al could not ®nd any difference in the concentration of circulating HCV RNA between samples collected in plasma tubes (EDTA or ACD) or serotubes [14].…”
Section: Discussionmentioning
confidence: 90%
“…Da Silva-Cardoso et al reported that after dilution and long-term storage (21 days at +4°C), the presence of HCV RNA was still detectable by using a sensitive qualitative test [5]. In a further study, these authors suggest that the presence of blood cells and the type of sample (serum or plasma) have only a small effect on HCV RNA concentration after storage at +4°C for 168 h [6]. No data are available concerning the effects of multiple cycles of freezing and thawing.…”
Section: Discussionmentioning
confidence: 91%
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“…Furthermore, it has to be noted that our validation was performed exclusively with plasma samples, and results cannot be transferred directly to serum samples. Interestingly, a study by Da Silva Cardoso et al [22] suggests that the presence of blood cells and the type of blood sample (serum or plasma) has only a small effect on the HCV RNA concentration after storage at 4 ° C.…”
Section: Discussionmentioning
confidence: 99%