PCR-RFLP of bone morphogenetic protein 15 (BMP15/FecX) gene as a candidate for prolificacy in sheep

Nagdy, Hiam; Mahmoud, Karima Gh. M.; Kandiel, Mohamed M.M.; Helmy, Nadia; Ibrahim, Shawky S.; Nawito, M.; Othman, Othman E.

doi:10.1016/j.ijvsm.2018.01.001

Cited by 12 publications

(16 citation statements)

References 25 publications

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“…However, the mutations reported in the TGF-β genes are not present in all sheep-producing meat breeds, including hair sheep breeds from African origin, such as D’man [8], Afshari, Ghezel, Lori-Bakhtyari and Afshari breeds from Iran [9], Grivette from France [10] and Black Belly from Mexico [11]. The absence of these mutations in these sheep breeds, could indicate that there are not unique genes that intervene in litter size [12], and there may be other molecular mechanisms involved in the prolificacy of sheep.…”

Section: Introductionmentioning

confidence: 99%

RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

Hernández-Montiel

Collí-Dulá

Ramón-Ugalde

et al. 2019

Genes

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The Pelibuey sheep (Ovis aries) is an indigenous breed distributed in the tropical regions of Mexico. The prolificacy of this sheep is on average from 1 to 1.5 lambs, being an important breeding characteristic that owners seek to increase with the purpose of economic improvements. New-generation RNA sequencing technology has been used to identify the genes that are expressed in the ovarian tissue of sheep that have two or more lambs per parturition, as well as to elucidate the metabolic pathways that are affected by the expression of these genes, with the purpose of better understanding the prolificacy in the sheep. In the present study, the transcriptional expression of multiparous and uniparous sheep was compared using RNA sequencing. Multiparous (M group) and uniparous (U group) sheep that had a genealogical record for three generations (M, n = 5 and U, n = 5) were selected. RNA was extracted from ovarian tissue and subsequently used to prepare the libraries that were sequenced using the Illumina NextSeq500 platform. A total of 31,575 genes were detected from the transcriptomic analysis of which 4908 were significantly expressed (p-value ≤ 0.001) in the ovary of sheep. Subsequently, a second filter was carried out to evaluate the false discovery rate (FDR) and select those genes with p-values ≤ 0.05 and values of expression ≥ 1 (log2), obtaining 354 differential expressed genes (DEG): 120 genes up-regulated and 234 genes down-regulated in the group M with respect to the group U. Through Gene Ontology (GO) and metabolic analysis, we obtained information on the function of differentially expressed genes, and its importance in the reproduction of multiparous sheep. This result suggest that genes identified in the present study participate in the development of the final stages of follicles.

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Section: Introductionmentioning

confidence: 99%

RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

Hernández-Montiel

Collí-Dulá

Ramón-Ugalde

et al. 2019

Genes

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“…BMP15 is an X chromosomal gene known as the FecX gene (fecundity X gene) that is associated with litter size (Lassoued et al, 2017). Given the large effect of BMP15 mutations on ovulation and litter size, it can be regarded as a major gene for reproduction in farm animals (Nagdy et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we created a PRISMA diagram (Fig. 1), which records the starting information from the studies and leads to the final dataset (Nakagawa et al, 2017).…”

Section: Characteristics Of Included Studiesmentioning

confidence: 99%

Meta-analysis of association between c.963A>G single-nucleotide polymorphism on BMP15 gene and litter size in goats

et al. 2022

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Abstract. Litter size is an important economic trait in the goat industry. Previous studies on the bone morphogenetic protein 15 (BMP15) gene detected some single-nucleotide polymorphisms (SNPs) such as c.963A>G that were associated with an increase in ovulation rate and litter size. The aim of this study was to conduct a meta-analysis on the effect of this polymorphism on litter size. We gathered and pooled data from five eligible published studies. To investigate the effect of c.963A>G on litter size, we utilized four different genetic models assuming dominant (GG + GA vs. AA), recessive (GG vs. GA + AA), additive (GG vs. AA) and co-dominant (GG + AA vs. GA) model of inheritance. Data were analyzed under random-effects models based on the I2 value. Furthermore, sensitivity analysis was carried out to validate the stability of results. The results showed that the c.963A>G polymorphism is associated with litter size when applying a dominant model (standardized mean difference (SMD) is 0.815, 95 % CI [0.170, 1.461], P value = 0.013) and also with an additive model (SMD = 0.755, 95 % CI [0.111, 1.400], P value = 0.022). However, the effect of c.963A>G polymorphism was not significant under recessive (SMD = 0.186, 95 % CI [−0.195, 4.259], P value = 0.339) and co-dominant (SMD = −0.119, 95 % CI [−0.525, 0.288], P value = 0.568) models. Sensitivity analysis demonstrated that dropping studies with wide confidence intervals affects overall results under the assumption of an additive model. The meta-analysis results revealed that the AA genotype could be positively connected with litter size in goats.

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“…With reference to previous research [ 6 , 8 , 10 ], four pairs of primers were designed for FecXI, FecXH, FecXG, and FecXB to detect the BMP15 gene. These primers are listed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…The following three classes of fecundity genes ( Fec ) have been identified in sheep: bone morphogenetic protein receptor type IB ( BMPR-IB ), known as FecB on chromosome 6, growth differentiation factor 9 ( GDF9 ), known as FecG on chromosome 5, and Bone Morphogenetic Protein 15 ( BMP15 ), known as FecX on the X chromosome. Interestingly, all three Fec genes belong to the TGF-β super family [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Identification of Mutation in BMP15 Gene Associated with Litter Size in Xinjiang Cele Black Sheep

Niu

Qiu

Jiang

et al. 2021

Animals

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The Bone Morphogenetic Protein 15 (BMP15) gene is known to have multiple single-nucleotide polymorphism sites associated with sheep fecundity. This study used gene sequence analysis and mutation detection assays for BMP15 by using 205 blood samples of ewes with known lambing records. Sequence analysis showed that mutation B1 missed the CTT base in exon 1 at positions 28–30, leading to a leucine deletion in the BMP15 protein. Litter size of ewes differed significantly between BB and B+ genotypes of B1 (p < 0.05); however, the differences between wild genotype (++) and homozygous (BB) or wild genotype (++) and heterozygous (B+) were not significant (p > 0.05). Another mutation, T755C, is a T-to-C base change at position 755 of exon 2, resulting in leucine replacement by proline at this position of the BMP15 protein (p.L252P). Two genotypes were identified in the flock: heterozygous (E+) and wild-type genotype (++). Ewes with heterozygous (E+) p.L252P had significantly larger litter sizes than those with the wild-type genotype (p < 0.05). Comprehensive analysis suggests that p.L252P is a mutation that affects fecundity in Cele black sheep.

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PCR-RFLP of bone morphogenetic protein 15 (BMP15/FecX) gene as a candidate for prolificacy in sheep

Cited by 12 publications

References 25 publications

RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

Meta-analysis of association between c.963A>G single-nucleotide polymorphism on BMP15 gene and litter size in goats

The Identification of Mutation in BMP15 Gene Associated with Litter Size in Xinjiang Cele Black Sheep

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