PCR-RFLP identification of four Chinese soft-shelled turtle<i>Pelodiscus sinensis</i>strains using mitochondrial genes

Zhang, Chao; Xu, Xiuhong; Zhang, Haiqi; Mu, Chang-Kao; He, Zhonghu

doi:10.3109/19401736.2013.869674

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…Hawaii haplotypes HM1 and HM2 were found in wild-caught and farm-bred samples of P. maackii from Japan and not in any other native range samples (Figure 8). They were also similar to the JP breeding strain, a farm-bred lineage present in Chinese turtle farms that purportedly originates from Japan ( Figure 9; Zhang et al 2014). Although it is unclear whether or not the softshell populations in Japan are indigenous (Stejneger 1907, Nakamura and Ueno 1963, Fritz et al 2010, Suzuki and Hikida 2014, it is established that Pelodiscus has occurred in Japan since at least the early 1800s (Temminck and Schlegel 1835).…”

Section: Inferences From Genetic Analysesmentioning

confidence: 67%

Origins of softshell turtles in Hawaii with implications for conservation

2015

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Section: Inferences From Genetic Analysesmentioning

confidence: 67%

Origins of softshell turtles in Hawaii with implications for conservation

2015

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“…According to the geographical distribution, P. sinensis can be divided into different strains, including the Japanese strain, Yellow River strain, Huaihe River strain, Poyang Lake strain, Taihu Lake strain, Southwest strain, Taiwan strain, etc. [ 6 ]. In recent years, two new strains of P. sinensis (Qingxi Huabie and Wubie strains) have been identified, which originated from Taihu Lake basin and are unique local strains in Zhejiang Province [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, two new strains of P. sinensis (Qingxi Huabie and Wubie strains) have been identified, which originated from Taihu Lake basin and are unique local strains in Zhejiang Province [ 7 ]. Different P. sinensis strains have their own traits, such as fast growth rate for the Japanese strain and light-yellow body color for the Yellow River strain [ 6 ]. With the continuous improvement in life quality, the consumption demand for P. sinensis has also been increasing.…”

Section: Introductionmentioning

confidence: 99%

“…Chen et al used the mitochondrial 12S rRNA to identify P. axenaria as a new species [ 24 ]. Zhang et al sequenced and analyzed the partial sequences of nad4 , cox1 , nad5 , and nad6 genes and further confirmed the identification of P. sinensis strains [ 6 ]. However, most studies only involved some gene fragments of the mitochondrial genome.…”

Section: Introductionmentioning

confidence: 99%

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Complete Mitochondrial Genomes of Four Pelodiscus sinensis Strains and Comparison with Other Trionychidae Species

Chen

Jiao

Yuan

et al. 2023

Biology

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The Chinese soft-shelled turtle (Pelodiscus sinensis) is an important aquaculture reptile with rich nutritional and medicinal values. In recent decades, the wild resources of P. sinensis have been depleting due to natural and artificial factors. Herein, we report the complete mitochondrial genome of four P. sinensis strains, including the Japanese (RB) strain, Qingxi Huabie (HB) strain, Jiangxi (JB) strain, and Qingxi Wubie (WB) strain. The nucleotide composition within the complete mitogenomes was biased towards A + T with a variable frequency ranging from 59.28% (cox3) to 70.31% (atp8). The mitogenomes of all four strains contained 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, 1 control region, and a replication origin region of the L-strand replication (OL), which was consistent with most vertebrates. Additionally, the atp8, nad4l, nad6, and nad3 genes possessed high genetic variation and can be used as potential markers for the identification of these P. sinensis strains. Additionally, all PCGs genes were evolving primarily under purifying selection. Through comparative analysis, it was revealed that most of the tRNAs were structurally different in the TψC stem, DHU stem, and acceptor stem. The length of the tandem repeats in the control region was variable in the four P. sinensis strains, ranging from 2 bp to 50 bp. Phylogenetic analysis indicated that all P. sinensis strains clustered into one branch and were closely related to other Trionychinae species. Overall, this study provides mitochondrial genome information for different P. sinensis strains to support further species identification and germplasm resource conservation.

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“…A germplasm bank for soft-shelled turtle was established with material collected from different geographical locations that included Taihu populations, Yellow River populations, Japanese strains, Qingxi black turtle, and other strains (He, 2014). As the morphological characteristics of some populations are similar, some rapid and accurate molecular differentiation methods such as RFLP-PCR (Zhang et al, 2015a) and SNP-HRM (Zhang et al, 2015b) were established to assist identification of the different populations/strains. Based on the characteristics of P. sinensis germplasm resources, Qingxi black turtle and Japanese strain were cultivated and authorized the new breed certificated by Ministry of Agriculture of the China (Zhang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Research Article A comparative study on genetic characteristics of two new varieties of Pelodiscus sinensis and their hybrid.

Zhang¹,

Xu²,

He³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Chinese soft-shelled turtle Pelodiscus sinensis has been an important aquaculture species in Southeast Asian countries. To breed a new variety of soft-shelled turtle with excellent properties and to evaluate the effect of hybridization of two turtle strains with a highly different trait phenotype, inheritance, microsatellite loci, and transcriptome analysis were studied in the hybrid turtles and their parents of P. sinensis Japanese strain and Qingxi black turtle. The genotypic characteristics and economic trait of the hybrid turtles were analyzed and compared to the two parents, showing significant growth vigor. The chromosome number of the hybrid turtle was diploid (2N = 66). The karyotype formulae were 8m+10sm+26t+22mc, with little differences between the two parents. Genotypic segregations of 241 microsatellite loci were screened in 3 populations including 90 species and showed that the specific allele numbers and polymorphic fragments increased in hybrid turtles indicating genetic diversity increased by hybridization. The liver transcriptome analysis of the hybrids and two parents showed similar distribution abundance in the parental and hybrid groups, but the transcripts with high abundance appeared in the hybrid group. There were 274 significant differentially expressed transcripts in the hybrid group compared to the two parental groups, among them 7 differentially expressed genes indicating superparent expression, and only 2 genes showing low-parent expression. In the differentially expressed genes, expression changes were mainly contributed to regulatory region changes rather than coding region sequences. These results would be important for facilitating successful breeding strategies by hybridization in P. sinensis.

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PCR-RFLP identification of four Chinese soft-shelled turtlePelodiscus sinensisstrains using mitochondrial genes

Cited by 7 publications

References 10 publications

Origins of softshell turtles in Hawaii with implications for conservation

Origins of softshell turtles in Hawaii with implications for conservation

Complete Mitochondrial Genomes of Four Pelodiscus sinensis Strains and Comparison with Other Trionychidae Species

Research Article A comparative study on genetic characteristics of two new varieties of Pelodiscus sinensis and their hybrid.

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