PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes

Toševski, Ivo; Jović, Jelena; Krstić, Oliver; Gaßmann, A.

doi:10.1007/s10526-013-9506-2

Cited by 16 publications

(13 citation statements)

References 22 publications

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“…Mecinus janthiniformis exhibits a strong host preference for DT and does not readily establish on YT in the field. A recently confirmed cryptic congener, the YT stem-mining weevil M. janthinus, shows promise as a selective biocontrol agent for YT (Toševski et al 2011(Toševski et al , 2013. Cryptic hybridization resulting in apparent YT or DT populations containing introgressed genetic material from the other species could make such populations unacceptable as hosts for their presumed Mecinus species and may explain why at some locations of presumed DT invasion, repeated releases of M. janthiniformis, in particular, have been unsuccessful (S.E.S., personal observation).…”

Section: Resultsmentioning

confidence: 99%

Plastid DNA Analysis Reveals Cryptic Hybridization in Invasive Dalmatian Toadflax (Linaria dalmatica) Populations

Boswell

Sing

Ward

2016

Invasive plant sci. manag.

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Gene flow between Dalmatian toadflax (DT) and yellow toadflax (YT), both aggressive invaders throughout the Intermountain West, is creating hybrid populations potentially more invasive than either parent species. To determine the direction of gene flow in these hybrid populations, species-diagnostic cytoplasmic markers were developed. Markers were based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) polymorphisms in the trnT-D chloroplast DNA (cpDNA) region digested with Alu1, and single-nucleotide polymorphisms (SNPs) in the matK and trnL-F chloroplast-barcoding regions. Four hybrid toadflax populations sampled from Colorado, Montana, and Washington contained both DT and YT cytoplasm, with YT predominating; 25 individuals from a fifth hybrid population from Idaho all had identical YT cpDNA haplotypes. Thirteen plants from two Colorado populations, assumed to be DT based on morphology and geographic isolation from any known YT population, were found to have YT cpDNA haplotypes. These results indicate that gene flow between invasive YT and DT populations is more widespread that previously realized and confirms that cryptic introgression of YT alleles has occurred in multiple western U.S. DT populations. The presence of YT genetic material in presumed DT populations may negatively affect host recognition and establishment by biocontrol agents used for toadflax management. Nomenclature: Dalmatian toadflax, Linaria dalmatica (L.) P. Mill.; yellow toadflax, Linaria vulgaris P. Mill.

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Section: Resultsmentioning

confidence: 99%

Plastid DNA Analysis Reveals Cryptic Hybridization in Invasive Dalmatian Toadflax (Linaria dalmatica) Populations

Boswell

Sing

Ward

2016

Invasive plant sci. manag.

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“…Few biological control agents for the control of invasive alien plants are screened using genetic techniques prior to release but there are some records of cryptic species being discovered in biological control agent populations or in populations of insects being considered as potential agents (Tošveski et al. ). Morphologically identical populations of the flea beetle, Psylliodes chalcomerus (Illiger) [Coleoptera: Chrysomelidae], a potential biological control agent for yellow starthistle, Centaurea solstitialis L. [Asteraceae], having a variety of different host plants in the indigenous range, were all regarded as a single species but were divided into three possible cryptic species using genetic analyses (Gaskin et al.…”

Section: Discussionmentioning

confidence: 99%

“…Tošveski et al. () also identified cryptic species within the indigenous range of the Mecinus janthinus Germar [Coleoptera: Curculionidae] species complex, a weevil introduced into North America as a biological control agent for two species of toadflax ( Linaria spp. [Scrophulariaceae]).…”

Section: Discussionmentioning

confidence: 99%

“…Whether the cryptic species of M. janthinus are present in the introduced populations in North America has not been confirmed (Tošveski et al. ). A possible cryptic species of the flea beetle, Aphthona lacertosa Rosenheim [Coleoptera: Chrysomelidae], a biological control agent for leafy spurge, Euphorbia esula L. [Euphorbiaceae], was identified using genetic techniques after its release in North America (Roehrdanz et al.…”

Section: Discussionmentioning

confidence: 99%

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Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments

Paterson

Mangan

Downie

et al. 2016

Ecology and Evolution

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There are many examples of cryptic species that have been identified through DNA‐barcoding or other genetic techniques. There are, however, very few confirmations of cryptic species being reproductively isolated. This study presents one of the few cases of cryptic species that has been confirmed to be reproductively isolated and therefore true species according to the biological species concept. The cryptic species are of special interest because they were discovered within biological control agent populations. Two geographically isolated populations of Eccritotarsus catarinensis (Carvalho) [Hemiptera: Miridae], a biological control agent for the invasive aquatic macrophyte, water hyacinth, Eichhornia crassipes (Mart.) Solms [Pontederiaceae], in South Africa, were sampled from the native range of the species in South America. Morphological characteristics indicated that both populations were the same species according to the current taxonomy, but subsequent DNA analysis and breeding experiments revealed that the two populations are reproductively isolated. Crossbreeding experiments resulted in very few hybrid offspring when individuals were forced to interbreed with individuals of the other population, and no hybrid offspring were recorded when a choice of mate from either population was offered. The data indicate that the two populations are cryptic species that are reproductively incompatible. Subtle but reliable diagnostic characteristics were then identified to distinguish between the two species which would have been considered intraspecific variation without the data from the genetics and interbreeding experiments. These findings suggest that all consignments of biological control agents from allopatric populations should be screened for cryptic species using genetic techniques and that the importation of multiple consignments of the same species for biological control should be conducted with caution.

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“…The P. longifila populations living on other plants are possibly a complex of cryptic species, rather than a single polyphagous species (EPPO 2015). Similar morphologically indistinguishable cryptic species pose significant challenges in other weed biological control projects (Mound et al 2010;Toševski et al 2011Toševski et al , 2013Rafter et al 2013;Paterson et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Host Associations of Gall-InducingProdiplosis longifila(Diptera: Cecidomyiidae) from Bolivia: Implications for Its Use as a Biological Control Agent forJatropha gossypiifolia(Euphorbiaceae)

Dhileepan¹,

Neser

Rumiz

et al. 2017

Florida Entomologist

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PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes

Cited by 16 publications

References 22 publications

Plastid DNA Analysis Reveals Cryptic Hybridization in Invasive Dalmatian Toadflax (Linaria dalmatica) Populations

Plastid DNA Analysis Reveals Cryptic Hybridization in Invasive Dalmatian Toadflax (Linaria dalmatica) Populations

Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments

Host Associations of Gall-InducingProdiplosis longifila(Diptera: Cecidomyiidae) from Bolivia: Implications for Its Use as a Biological Control Agent forJatropha gossypiifolia(Euphorbiaceae)

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