PCR Methods and Approaches

Phylogenetic relationships of the largest lungless tetrapod (Gymnophiona, Atretochoana) and the evolution of lunglessness in caecilians

“…); seven in absentia (SIA1 T3 and SIA2 T7 in Bonacum et al . ); and two fragments of the member 3 of the solute carrier family 8 (part1: Slc8a3‐E and Slc8a3‐B; part 2: Slc8a3‐C and Slc8a3‐J in Roelants et al . ).…”

“…Taxon sampling and DNA data set Our data set is composed of 21 taxa (Table 1) We used available data in GenBank for eleven taxa, and we produced sequences for ten species (Table 1). We used fragments of three mitochondrial DNA genes: 12S (L1091 and H1478 in Kocher et al 1989);16S (16Sar-L and 16Sbr-H in Palumbi et al 1991); cytochrome c oxidase subunit 1 (Amp-P3 F and Amp-P3 R in San Mauro et al 2004); and five nuclear genes: chemokine receptor type 4 (CXCR4-N and CXCR4-L in Roelants et al 2007); histone H3 (H3F and H3R in Colgan et al 1999); recombinationactivating gene 1 (Amp-RAG1 F and Amp-RAG1 R1 in San Mauro et al 2004); seven in absentia (SIA1 T3 and SIA2 T7 in Bonacum et al 2001); and two fragments of the member 3 of the solute carrier family 8 (part1: Slc8a3-E and Slc8a3-B; part 2: Slc8a3-C and Slc8a3-J in Roelants et al 2007).…”

“…Polymerase chain reaction (PCR) was carried out in 14 lL reactions using the kit (5U/lL, Promega, Madison-WI USA). Amplifications were carried out with specific protocols for each primer (Kocher et al 1989;Palumbi et al 1991;Colgan et al 1999;Bonacum et al 2001;San Mauro et al 2004;Roelants et al 2007) and the sequencing reaction with the Kit BigDye (Applied Biosystems). The sequences were generated on a sequencer ABI 3500 (Applied Biosystems) and edited with BioEdit (Hall 1999).…”