PCR Method To Identify
            <i>Salmonella enterica</i>
            Serovars Typhi, Paratyphi A, and Paratyphi B among
            <i>Salmonella</i>
            Isolates from the Blood of Patients with Clinical Enteric Fever

Levy, Haim; Diallo, Souleymane; Tennant, Sharon M.; Livio, Sofie; Sow, Samba O.; Tapia, Milagritos D.; Fields, Patricia I.; Mikoleit, Matthew; Tamboura, Boubou; Kotloff, Karen L.; Lagos, Rosanna; Nataro, James P.; Galen, James E.; Levine, Myron M.

doi:10.1128/jcm.00109-08

Cited by 107 publications

(106 citation statements)

References 25 publications

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“…However, initial research indicated that PCR has similar sensitivity to blood culture and lower specificity [15]. A prospective study of the concentration of S. Typhi in blood of typhoid fever patients showed a median value of 0.3 (range of 0.1 to 399) CFU/ml, well below current PCR-based detection limits [16].…”

Section: Discussionmentioning

confidence: 96%

Detection of Salmonella in Blood by PCR using iroB gene

Ganesan

Harish

Menezes

et al. 2014

JCDR

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Section: Discussionmentioning

confidence: 96%

Detection of Salmonella in Blood by PCR using iroB gene

Ganesan

Harish

Menezes

et al. 2014

JCDR

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“…Herrera-Leon et al (2007) [7] had developed a multiplex PCR identifying serogroups B, C1, C2, D and E. In the O-grouping multiplex PCR developed by Levy et al (2008) [22], only serogroups A, B, D and Vi-positive strains were identified. We combined both these systems to cover a wider range of major serogroups (A, B, C1, D and E as well as Vi-positive strains) to enhance the versatility of the system.…”

Section: Discussionmentioning

confidence: 99%

“…One way to avoid this serological cross-reactivity is to use DNA-based methods for serotyping, such as PCR, which does not involve antigens or antibodies. Various PCR tests have been developed for species identification of Salmonella enterica [1,4,6,7,22,[24][25][26][27].…”

Section: Discussionmentioning

confidence: 99%

“…Conventional serotyping (Table 1). Primers used in H typing multiplex PCR were from Levy et al (2008) [22] to detect H1 antigen "a", "b" and "d" (Table 1). Internal controls (P1-P2 primers that amplify oriC) were incorporated to avoid false negative result [22,33].…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…These molecular methods, such as multiplex PCR, are highly sensitive, very specific, fast and reproducible. Such approaches have previously been reported by others [1,4,6,7,14,19,22,[24][25][26][27]29]. In Salmonella, the genes responsible for biosynthesis of the O antigens are normally grouped together on the chromosome in a gene cluster called rfb [6].…”

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confidence: 99%

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Application of PCR-based serogrouping of selected Salmonella serotypes in Malaysia

Lim

Thong

2009

J Infect Dev Ctries

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Background: Differentiation of Salmonella enterica into its serogroups is important for epidemiological study. The objective of the study was to apply a multiplex PCR targeting serogroups A, B, C1, D, E and Vi-positive strains of Salmonella enterica commonly found in Malaysia. A separate H-typing multiplex PCR which identified flagellar antigen "a", "b" or "d" was also optimized to confirm clinical serotypes, S. Paratyphi A and S. Typhi. Methodology: Sixty-seven laboratory Salmonella enterica strains were tested. Six sets of primers targeting defined regions of the O antigen synthesis genes (rfb gene cluster) and Vi antigen gene (viaB) were selected and combined into a multiplex PCR for O-grouping. Four primers (H-for, Ha-rev, Hb-rev and Hd-rev) were used in the second step multiplex PCR for H-typing. The optimized mPCR assays were further evaluated with 58 blind-coded Salmonella strains. Results: The multiplex PCR results obtained showed 100% concordance to the conventionally typed serogroups. Validation with 58 blind coded Salmonella strains yield 100% accuracy and specificity. Conclusion: Based on this study, PCR serogrouping proved to be a rapid, alternative method for further differentiation of Salmonella enterica.

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Molecular identification in monophasic and nonmotile variants of Salmonella enterica serovar Typhimurium

et al. 2012

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Variant strains of Salmonella enterica serovar Typhimurium, lacking one or both flagellar phases have been widely reported. The monophasic S.1,4,[5],12:i:- variant has emerged worldwide in the past few years and has become one of the most frequently encountered in many countries. In contrast, monophasic S.1,4,[5],12:-:1,2 and nonmotile S.1,4,[5],12:-:- strains are rarely described. This study investigated seven molecular markers to identify and delineate monophasic S.1,4,[5],12:i:- (n = 90), S.1,4,[5],12:-:1,2 (n = 25), nonmotile S.1,4,[5],12:-:- (n = 17) strains, and some serovar Typhimurium strains (n = 124) collected through the French Salmonella network between 2001 and 2010. Three markers were commonly detected in serovar Typhimurium and in all variant strains: STM2757, mdh and fliA-B. Monophasic S.1,4,[5],12:i:- were genotypically confirmed by the absence of the fljB, fljA, and hin genes. Nevertheless, 13 (14.5%) of them were positive for these last three genes, revealing monophasic strains named “inconsistent” as previously described. All nonmotile 1,4,[5],12:-:- strains had the fliC, fljA, fljB, and hin genes and the fliC gene was detected in 88% of monophasic S.1,4,[5],12:-:1,2 strains. The combination of the seven markers detection enables to recognize eight different genotypes within the S.1,4,[5],12:i:- collection, among which the Spanish and the U.S. clones previously described could be distinguished and assigned to a genotype. Based on this molecular approach, 71% of the French S.1,4,[5],12:i:- collection belonged to the Spanish clone, whereas only 2% were assigned to the U.S. clone. This study highlights the usefulness of these molecular markers and genotypes for identifying lineages, especially among the epidemiologically important monophasic S.1,4,[5],12:i:- variant.

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PCR Method To Identify Salmonella enterica Serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the Blood of Patients with Clinical Enteric Fever

Abstract: PCR methodology was developed to identify

Cited by 107 publications

References 25 publications

Detection of Salmonella in Blood by PCR using iroB gene

Detection of Salmonella in Blood by PCR using iroB gene

Application of PCR-based serogrouping of selected Salmonella serotypes in Malaysia

Molecular identification in monophasic and nonmotile variants of Salmonella enterica serovar Typhimurium

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