PCR-Mediated Amplification of Group A Streptococcal Genes Encoding Immunoglobulin-Binding Proteins

Podbielski, Andreas; Kaufhold, Achim; Cleary, P P

doi:10.1006/immu.1993.1007

Cited by 27 publications

(34 citation statements)

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“…The SF4 genes derived from type 11 and 13 strains are closely related to one another, displaying >95% nucleotide sequence identity throughout their 5' 147 bp, suggesting that the type 11 and 13 strain genes represent a divergent form of the type 49 strain SF4 gene. Our nucleotide sequence for the SF4 gene derived from a type 49 strain is largely in agreement with the recently reported sequence for fcrA49 (19). A phylogenetic tree was constructed based on nucleotide sequence alignments between the 5' ends of the SF4 emm genes (Fig.…”

Section: Resultssupporting

confidence: 85%

Allelic polymorphism of emm loci provides evidence for horizontal gene spread in group A streptococci.

Bessen

Hollingshead

1994

Proc. Natl. Acad. Sci. U.S.A.

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Section: Resultssupporting

confidence: 85%

Allelic polymorphism of emm loci provides evidence for horizontal gene spread in group A streptococci.

Bessen

Hollingshead

1994

Proc. Natl. Acad. Sci. U.S.A.

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“…The different M serotype GAS strains used in this study have been described by Podbielski et al (58,59) and by Kaufhold et al (60). The Escherichia coli strain DH5␣ was purchased from Invitrogen and served as a host for plasmids pFW5-luc (Podbielski et al (51)), pUCerm (Baev et al (71)), and pMAL-c2 (New England Biolabs, Frankfurt/Main, Germany).…”

Section: Methodsmentioning

confidence: 99%

Streptococcus pyogenes Collagen Type I-binding Cpa Surface Protein

Kreikemeyer¹,

Nakata

Oehmcke

et al. 2005

Journal of Biological Chemistry

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The Streptococcus pyogenes collagen type I-binding protein Cpa (collagen-binding protein of group A streptococci) expressed by 28 serotypes of group A streptococci has been extensively characterized at the gene and protein levels. Evidence for three distinct families of cpa genes was found, all of which shared a common sequence encoding a 60-amino acid domain that accounted for selective binding to type I collagen. Surface plasmon resonance-based affinity measurements and functional studies indicated that the expression of Cpa was consistent with an attachment role for bacteria to tissue containing collagen type I. A cpa mutant displayed a significantly decreased internalization rate when incubated with HEp-2 cells but had no effect on the host cell viability. By utilizing serum from patients with a positive titer for streptolysin/DNase antibody, an increased anti-Cpa antibody titer was noted for patients with a clinical history of arthritis or osteomyelitis. Taken together, these results suggest Cpa may be a relevant matrix adhesin contributing to the pathogenesis of S. pyogenes infection of bones and joints.

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“…This strain is a patient isolate originally obtained from R. Lütticken, Aachen, Germany. GAS strains of M serotypes 1,2,3,4,5,6,8,9,11,12,13,14,17,18,19,22,28,41,42,46,47,48,49,52,53,55, and 57 were as described by Podbielski et al (41). The M-nontypeable GAS strain 64/14 was provided by M. D. P. Boyle, Toledo, Ohio.…”

Section: Methodsmentioning

confidence: 99%

Involvement of Lsp, a Member of the LraI-Lipoprotein Family inStreptococcus pyogenes, in Eukaryotic Cell Adhesion and Internalization

Elsner¹,

Kreikemeyer²,

Braun-Kiewnick³

et al. 2002

Infect Immun

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Three open reading frames (ORFs) were identified by a genome walking strategy in the genomes of serotype M49 group A streptococcal (GAS) strains CS101 and 591. These ORFs were located between the mga core regulon and the dipeptide permease operon. The deduced amino acid (aa) sequences contained signature sequences indicative of a lipoprotein (306 aa), an intracellular protein (823 aa), and a secreted peptide (66 aa), respectively. ORF1 (named Lsp for lipoprotein of Streptococcus pyogenes) and ORF2 exhibited a high degree of homology to the lmb/ORF2 genes of S. agalactiae (B. Spellerberg et al., Infect. Immun. 67:871-878, 1999). The three ORFs were found to be present in each of the 27 GAS serotype strains tested. Transcription analysis revealed a polycistronic lsp/ORF2 and a monocistronic ORF3 message that were detected primarily at the transition from exponential to stationary growth phase. lsp and ORF2 mutants, ORF2-and ORF3-luciferase reporter fusions, and antiserum against recombinant Lsp were produced to examine the biological role of these genes. Although high Zn 2؉ and Cu 2؉ ion concentrations decreased lsp operon expression, Lsp did not transport divalent cations as described for other LraI-type operons. The lsp mutant had reduced fibronectin binding. Although no direct binding of Lsp to fibronectin could be demonstrated, the lsp mutant showed decreased transcription of prtF2 encoding the fibronectin-binding protein F2. Both the lsp and ORF2 mutants showed decreased laminin binding. Adherence to and internalization into A549 epithelial cells of both mutants was reduced without a detectable effect on eukaryotic cell viability. The transcription of a number of virulence factors was altered in the lsp mutants and ORF2 mutants. The changes in laminin binding and eukaryotic cell internalization could be explained by changes in transcription of speB (cysteine protease) and/or the global regulators mga, csrRS, and nra.

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PCR-Mediated Amplification of Group A Streptococcal Genes Encoding Immunoglobulin-Binding Proteins

Cited by 27 publications

References 0 publications

Allelic polymorphism of emm loci provides evidence for horizontal gene spread in group A streptococci.

Allelic polymorphism of emm loci provides evidence for horizontal gene spread in group A streptococci.

Streptococcus pyogenes Collagen Type I-binding Cpa Surface Protein

Involvement of Lsp, a Member of the LraI-Lipoprotein Family inStreptococcus pyogenes, in Eukaryotic Cell Adhesion and Internalization

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